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The map label for this gene is thiD [H]

Identifier: 227081467

GI number: 227081467

Start: 1332519

End: 1333367

Strand: Direct

Name: thiD [H]

Synonym: VCM66_1251

Alternate gene names: 227081467

Gene position: 1332519-1333367 (Clockwise)

Preceding gene: 227081463

Following gene: 227081470

Centisome position: 46.07

GC content: 49.23

Gene sequence:

>849_bases
ATGTCAATAATCTCTTCTATTCATACTCCCATAGTGCTGACTATCGCAGGCTCAGATAGCGGTGGTGGCGCTGGAATTCA
AGCCGATATCAAAGCCATTTCAGCGACAGGGGGGTACGCCTGCTCAGTGATTACCGCGATTACCGCACAAAATACGCAGG
GTGTTTCCGCCGTTTTTCCTTTGCCATTAGCGGTGGTTGAGCAACAGTTAGACGCCGTGTTTAGCGATATTCCCGTCTTG
GCAGTCAAAATTGGTATGCTGGCGGATGCCCCAATCATCGCTCTGGTTGCTAAGAAATTACGCCAATATCAACCGAAATG
GATTGTGCTCGACCCAGTGATGATCTCAACCAGTGGCCATCATTTATTGGCGGCAGAGGCAGTCGAGACGTTAAAACGTG
AGCTATTACCGTTAGCCAATCTCATTACGCCTAATCTGCCAGAAGCGGCGGTTTTAGCGGGGATGACGCAACAAGAATGG
CAGCAGAATCCGCAGCAGGGTATTACCGTGCTTAGTCAGTTAAACACACCAGCGGTACTGCTCAAAGGTGGTCACGACCA
TCAGCAAGCCAACAGTGATGACTTACTGATTCAAAGTGGTGATAGTCGTCATTTCTGTGCGCCCCGTATTGCTACGAAGA
ATACGCATGGCACGGGCTGCTCACTCTCATCTGCGATTGCTTCATACTTAGCGCAGGGGGCTGAGTTAGTGGAAGCGGTG
GATAAAGCCAAACAGTATATCCACCAAGCGATCGTGCATGCCGATCAATTAAGGATCGGCCAAGGTTATGGGCCGATACA
CCATTTTTATCTACTGCAAATGGCTAAAAGCCAGCAGAAAAGCCAATAA

Upstream 100 bases:

>100_bases
ATTCAGTTAGGACTGACGTAGGGAACTATCCTCAACCGCTGGTATTGTCTCACCATTGATTTGCAGTTTGGGATCAGTTC
CTGTTTTCACAGGAGCTTTT

Downstream 100 bases:

>100_bases
GAGTGAGCTTGAGTTATAAAAAAACCGTCTCGCTTGAGACGGTTTTTACATTCAACAGAGCGGAATTAGAAACTCGCGCT
GCGTGGGGTACGTGGGAATG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSIISSIHTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFPLPLAVVEQQLDAVFSDIPVL
AVKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGHHLLAAEAVETLKRELLPLANLITPNLPEAAVLAGMTQQEW
QQNPQQGITVLSQLNTPAVLLKGGHDHQQANSDDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAV
DKAKQYIHQAIVHADQLRIGQGYGPIHHFYLLQMAKSQQKSQ

Sequences:

>Translated_282_residues
MSIISSIHTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFPLPLAVVEQQLDAVFSDIPVL
AVKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGHHLLAAEAVETLKRELLPLANLITPNLPEAAVLAGMTQQEW
QQNPQQGITVLSQLNTPAVLLKGGHDHQQANSDDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAV
DKAKQYIHQAIVHADQLRIGQGYGPIHHFYLLQMAKSQQKSQ
>Mature_281_residues
SIISSIHTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFPLPLAVVEQQLDAVFSDIPVLA
VKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGHHLLAAEAVETLKRELLPLANLITPNLPEAAVLAGMTQQEWQ
QNPQQGITVLSQLNTPAVLLKGGHDHQQANSDDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAVD
KAKQYIHQAIVHADQLRIGQGYGPIHHFYLLQMAKSQQKSQ

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=48.6486486486487, Blast_Score=238, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=34.0579710144928, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=35.3571428571429, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324997, Length=286, Percent_Identity=33.9160839160839, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29865; Mature: 29734

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIISSIHTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFP
CCHHHHCCCCEEEEEECCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHCCCCCCCEEECC
LPLAVVEQQLDAVFSDIPVLAVKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGH
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCEEEECCCC
HLLAAEAVETLKRELLPLANLITPNLPEAAVLAGMTQQEWQQNPQQGITVLSQLNTPAVL
EEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCEEE
LKGGHDHQQANSDDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAV
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKAKQYIHQAIVHADQLRIGQGYGPIHHFYLLQMAKSQQKSQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIISSIHTPIVLTIAGSDSGGGAGIQADIKAISATGGYACSVITAITAQNTQGVSAVFP
CHHHHCCCCEEEEEECCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHCCCCCCCEEECC
LPLAVVEQQLDAVFSDIPVLAVKIGMLADAPIIALVAKKLRQYQPKWIVLDPVMISTSGH
CHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCEEEECCCC
HLLAAEAVETLKRELLPLANLITPNLPEAAVLAGMTQQEWQQNPQQGITVLSQLNTPAVL
EEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHCCHHHHHHHHHHCCCCEEE
LKGGHDHQQANSDDLLIQSGDSRHFCAPRIATKNTHGTGCSLSSAIASYLAQGAELVEAV
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKAKQYIHQAIVHADQLRIGQGYGPIHHFYLLQMAKSQQKSQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]