| Definition | Vibrio cholerae M66-2 chromosome I, complete genome. |
|---|---|
| Accession | NC_012578 |
| Length | 2,892,523 |
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The map label for this gene is rpfG [H]
Identifier: 227081466
GI number: 227081466
Start: 1330532
End: 1332010
Strand: Reverse
Name: rpfG [H]
Synonym: VCM66_1250
Alternate gene names: 227081466
Gene position: 1332010-1330532 (Counterclockwise)
Preceding gene: 227081468
Following gene: 227081465
Centisome position: 46.05
GC content: 48.34
Gene sequence:
>1479_bases TTGACCATTTGGGTTCTATCACTTGCTATGAAAAACACGATTTACCATGCCCCACTGACGTTTGGACTCTATGCGCTTGC TGGAGTGCTCTTTGCGCTTTACACAAGCCGAGTCTGTCCTTTTCTCGCGACATTAAGCACTCGTGAAATCGCCACTCAGG TGGGTGCTGTCTTCATCCTCGCATGGCTGATTCGGCATACTCTGCTTCGCCAGCACATTTGGGCAAGAAAACAGCAATTT ATCCAATTGGACACGGCACTCCTCTTCGCAATGAGCCTGCCTTTAGCGCTTTACTACAATCTGCAATACCAGTTCACGTT AGACAGTAATTTAAAAGTACTGTTTGGAATGACCTTATTTGGTTTCTTCACCGGAGCGTTATTGCAATTAAGCAGTAAGT TAAGAACGCTGCGCCAAATGCCTCAAAGTCAGAACCTTGCTCTCTCTTCTGATGAGCGACGTTCCTTAGTCAAACAGCTG ATTGGCTTGATCGTCCTTTTAATCAGCACACTGACAGTGATGCTCAGCATGGTCGCAATCAAAGATATTCACTGGCTGGA AAATAACCCTGCGCGCCTACTTGATGGCAGTGGCAAGATCAGCATTGTGAAAGAGTTTGCCTTTTTGTCCTTGGTGTTAG GCGGCTACATCACGGCCATTCTCGTACTCTGGAGTCGAATGATGAAAGAGATCCTCGACCATCAGGAGCGCTCATTGCAA GCCGTGACGCAAGGCAACCTCCAAGTACGCCTTCCCGTCTACTCTAACGATGAGTTAGGCAATGTCGCCATGTTGACCAA TCAGATGTTAGACAGCTTAGAAGCTACGCAAAATGAGGTCAAAACCACACGTGATGTGGCTATCGTCAGCTTATCGGCTC TTGCGGAATCACGAGATAACGAGACAGGGGCACATATTCTTCGCACTCAAGAATACGTCAAAGCGTTAGCAGAATATCTC GCTGCTTTTCCCCAATATTCAACGCTGTTAACCCCGGCTTATATCGAACTACTCTACAAATCCGCGCCATTGCATGATGT AGGCAAGGTTGGCATTCCCGATTCGGTTCTGCTCAAACCGGGCAAACTGACCGATGAGGAGTTCACCGTCATGAAAGAGC ATCCGCGTATCGGGGCGCAAGCACTTGCGATTGCTGAGCGCCATTTAGGGACGAGCTCCTTCCTCGCCATTGCCAAAGAA ATTGCTCTCACTCACCACGAAAAATGGGATGGCACGGGTTATCCGGCGCAATTACAAGGAGAAGCCATCCCGCTTTCCGG TCGCTTAATGGCGTTAGCCGATGTGTATGATGCCTTGATTTCAGCTCGGGTTTATAAACCGGCTTTTAGTCATGACAAAG CCAAAGCCATTATTGTCGAAGGCAGTGGTCACCATTTTGATCCTGCCGTGGTCGAGGCTTTTCTCGCGGTTGAGGAAAAG TTCGTAGCCATTGCAGCACACTTTAAAGATGCCGCATAA
Upstream 100 bases:
>100_bases ATCACCCAGCAATGTCTATAAAATCCGCGCCTTTATTCAAAATGATAATAAATCACACTCACTTTAATGACGCTGCTTAT ACTTAACTAGCCGTAACTTA
Downstream 100 bases:
>100_bases GGTTGTAGTTATACCCTTCCTACTTGAAGCTGCAGCGGTGTTGGCGACGCTCTGCTTTGCGAATGCTCAATCCATGACGA TGGAAAGAAATGAAAATTTA
Product: hypothetical protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 492; Mature: 491
Protein sequence:
>492_residues MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQF IQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQL IGLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYL AAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKE IALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK FVAIAAHFKDAA
Sequences:
>Translated_492_residues MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQF IQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQL IGLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYL AAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKE IALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK FVAIAAHFKDAA >Mature_491_residues TIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFILAWLIRHTLLRQHIWARKQQFI QLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLFGFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLI GLIVLLISTLTVMLSMVAIKDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQA VTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDNETGAHILRTQEYVKALAEYLA AFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKPGKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEI ALTHHEKWDGTGYPAQLQGEAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEKF VAIAAHFKDAA
Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 response regulatory domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011006 - InterPro: IPR003607 - InterPro: IPR001789 [H]
Pfam domain/function: PF00072 Response_reg [H]
EC number: NA
Molecular weight: Translated: 54566; Mature: 54435
Theoretical pI: Translated: 7.55; Mature: 7.55
Prosite motif: PS50885 HAMP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFIL CEEEEEEHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AWLIRHTLLRQHIWARKQQFIQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHH GFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLIGLIVLLISTLTVMLSMVAI HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ HHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDN HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC ETGAHILRTQEYVKALAEYLAAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKP CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC GKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEIALTHHEKWDGTGYPAQLQG CCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECC EAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH FVAIAAHFKDAA HHHHHHHHCCCC >Mature Secondary Structure TIWVLSLAMKNTIYHAPLTFGLYALAGVLFALYTSRVCPFLATLSTREIATQVGAVFIL EEEEEEHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AWLIRHTLLRQHIWARKQQFIQLDTALLFAMSLPLALYYNLQYQFTLDSNLKVLFGMTLF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEECCCCHHHHHHHHH GFFTGALLQLSSKLRTLRQMPQSQNLALSSDERRSLVKQLIGLIVLLISTLTVMLSMVAI HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH KDIHWLENNPARLLDGSGKISIVKEFAFLSLVLGGYITAILVLWSRMMKEILDHQERSLQ HHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH AVTQGNLQVRLPVYSNDELGNVAMLTNQMLDSLEATQNEVKTTRDVAIVSLSALAESRDN HHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC ETGAHILRTQEYVKALAEYLAAFPQYSTLLTPAYIELLYKSAPLHDVGKVGIPDSVLLKP CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECC GKLTDEEFTVMKEHPRIGAQALAIAERHLGTSSFLAIAKEIALTHHEKWDGTGYPAQLQG CCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECC EAIPLSGRLMALADVYDALISARVYKPAFSHDKAKAIIVEGSGHHFDPAVVEAFLAVEEK CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH FVAIAAHFKDAA HHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11123673 [H]