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The map label for this gene is celF [H]

Identifier: 227081456

GI number: 227081456

Start: 1316452

End: 1317774

Strand: Direct

Name: celF [H]

Synonym: VCM66_1239

Alternate gene names: 227081456

Gene position: 1316452-1317774 (Clockwise)

Preceding gene: 227081455

Following gene: 227081457

Centisome position: 45.51

GC content: 46.41

Gene sequence:

>1323_bases
ATGTCTCGAAATGCATTAAAGCTGGCAGTCATCGGCGGTGGCAGTAGTTACACCCCAGAGCTTGTGGAAGGGGTTCTCAA
AAGATTGGATTTTCTGCCAGTGAAGCAGTTGCACTTTGTGGATATTGAAGCCGGTGCGGAAAAATTACAGATCATTCGAG
ATCTTGCGCAGAGAATGGTCGACAAAGTTGGAGCTGACATTGAAATTAAAGCGGGCTTTGATCGCCGAGAGGCGATTGCC
GGCGCTGATTTCGTTATGACCCAGTTTCGGGTTGGGGGGCTTGCCGCTCGCGCGAATGATGAACGAATTCCACTGAAATA
TGATGTGATTGGGCAAGAAACCACAGGGCCTGGTGGTTTTGCCAAAGCACTTCGAACGATCCCAGTCATACTGGATATTT
GCCGAGATATTGAAGAGCTAGCACCCAATGCCTGGATGCTCAACTTTACCAATCCTGCGGGCTTGGTCTCGGAGGCAGTC
AGTAAATACTCAAAAGTCAAAAGCATAGGATTATGTAATGTTCCCGTTTCTATGCAGATGATGATTGCTGAAATGATGGC
CTGTGAACCTCAAGACCTTCAGCTCGAGTTCGCTGGGCTGAACCATTTAGTTTGGGTGCACCAAGCTTGGTTGGATGGTC
AAAATATCACTCAAACCGTTTTAGAAAAGGTGGGTGATGGCGCCAATTTCAGCATGAAAAATATCTGGGAAGAACCTTGG
GATCCTGAGTTCTTAAAAGCGTTAGGCGCGATTCCTTGCCCTTATCACCGCTACTTTTACCAAACGGATGCTATGTTGGC
AGAAGAGAAAAAAAGTGCTCAAGAGAAAGGAACGCGAGCTGAGCAAGTGATGGTCACTGAAAAGGCCTTATTTGAGCTCT
ATCAAGATCCACATCTGGCTCATAAGCCTAAAGAGTTAGAAGCGCGTGGCGGTGCTTATTATTCGGATGCCTCGTTAAAT
CTTGTTGATGCCATCTACAATAATCGCAATAGTATTCATGTGGTTAATGTGCAGAATCATGGGGCGATCAGTTCATTACC
TCATGATGCCGTGATTGAATGCAGTGCGGTGGTGGGCAGTTGGGGAGCAAAACCGATTGCGGTCGGAGAACTTTCACCAA
AAATCAGTGGATTGCTACATCAAGTGAAAGCCTATGAGCAGCTTGCGATAGAAGCCGCAGTACACGGTGACTATCACCTT
GCTTTAATGGCTTTAACGAATAACCCATTGGTGCCTGATATTGGTCGGGCGAAAGCCATTTTGGATGATATTCTGCGTGA
AAATGCCGTTTATCTACCCCAATTTAAACTCACCACATGGTAA

Upstream 100 bases:

>100_bases
TGGTGCATGCTCAAGATCATTTAATGACGACAATTCTGGCACAGGAAATGGCGACTGAAATGGTGGCTTTACATAAACAC
TTGGCGGGTGAGGAAGCAAA

Downstream 100 bases:

>100_bases
CGGTAATAAGGTGAATCTCTCGATGAAGGTAATTTTTAACGCGGACGATTTTGGTTTAACTCAAGGTGTCAACCAAGGTA
TTGTCAAGGCCCACCTTGAT

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSRNALKLAVIGGGSSYTPELVEGVLKRLDFLPVKQLHFVDIEAGAEKLQIIRDLAQRMVDKVGADIEIKAGFDRREAIA
GADFVMTQFRVGGLAARANDERIPLKYDVIGQETTGPGGFAKALRTIPVILDICRDIEELAPNAWMLNFTNPAGLVSEAV
SKYSKVKSIGLCNVPVSMQMMIAEMMACEPQDLQLEFAGLNHLVWVHQAWLDGQNITQTVLEKVGDGANFSMKNIWEEPW
DPEFLKALGAIPCPYHRYFYQTDAMLAEEKKSAQEKGTRAEQVMVTEKALFELYQDPHLAHKPKELEARGGAYYSDASLN
LVDAIYNNRNSIHVVNVQNHGAISSLPHDAVIECSAVVGSWGAKPIAVGELSPKISGLLHQVKAYEQLAIEAAVHGDYHL
ALMALTNNPLVPDIGRAKAILDDILRENAVYLPQFKLTTW

Sequences:

>Translated_440_residues
MSRNALKLAVIGGGSSYTPELVEGVLKRLDFLPVKQLHFVDIEAGAEKLQIIRDLAQRMVDKVGADIEIKAGFDRREAIA
GADFVMTQFRVGGLAARANDERIPLKYDVIGQETTGPGGFAKALRTIPVILDICRDIEELAPNAWMLNFTNPAGLVSEAV
SKYSKVKSIGLCNVPVSMQMMIAEMMACEPQDLQLEFAGLNHLVWVHQAWLDGQNITQTVLEKVGDGANFSMKNIWEEPW
DPEFLKALGAIPCPYHRYFYQTDAMLAEEKKSAQEKGTRAEQVMVTEKALFELYQDPHLAHKPKELEARGGAYYSDASLN
LVDAIYNNRNSIHVVNVQNHGAISSLPHDAVIECSAVVGSWGAKPIAVGELSPKISGLLHQVKAYEQLAIEAAVHGDYHL
ALMALTNNPLVPDIGRAKAILDDILRENAVYLPQFKLTTW
>Mature_439_residues
SRNALKLAVIGGGSSYTPELVEGVLKRLDFLPVKQLHFVDIEAGAEKLQIIRDLAQRMVDKVGADIEIKAGFDRREAIAG
ADFVMTQFRVGGLAARANDERIPLKYDVIGQETTGPGGFAKALRTIPVILDICRDIEELAPNAWMLNFTNPAGLVSEAVS
KYSKVKSIGLCNVPVSMQMMIAEMMACEPQDLQLEFAGLNHLVWVHQAWLDGQNITQTVLEKVGDGANFSMKNIWEEPWD
PEFLKALGAIPCPYHRYFYQTDAMLAEEKKSAQEKGTRAEQVMVTEKALFELYQDPHLAHKPKELEARGGAYYSDASLNL
VDAIYNNRNSIHVVNVQNHGAISSLPHDAVIECSAVVGSWGAKPIAVGELSPKISGLLHQVKAYEQLAIEAAVHGDYHLA
LMALTNNPLVPDIGRAKAILDDILRENAVYLPQFKLTTW

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=435, Percent_Identity=44.8275862068966, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790560, Length=457, Percent_Identity=26.0393873085339, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48513; Mature: 48382

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNALKLAVIGGGSSYTPELVEGVLKRLDFLPVKQLHFVDIEAGAEKLQIIRDLAQRMV
CCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DKVGADIEIKAGFDRREAIAGADFVMTQFRVGGLAARANDERIPLKYDVIGQETTGPGGF
HHHCCCEEEECCCCHHHHHCCCHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCCHH
AKALRTIPVILDICRDIEELAPNAWMLNFTNPAGLVSEAVSKYSKVKSIGLCNVPVSMQM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MIAEMMACEPQDLQLEFAGLNHLVWVHQAWLDGQNITQTVLEKVGDGANFSMKNIWEEPW
HHHHHHHCCCCCCEEEECCCCCEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCC
DPEFLKALGAIPCPYHRYFYQTDAMLAEEKKSAQEKGTRAEQVMVTEKALFELYQDPHLA
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
HKPKELEARGGAYYSDASLNLVDAIYNNRNSIHVVNVQNHGAISSLPHDAVIECSAVVGS
CCCCHHHCCCCCEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
WGAKPIAVGELSPKISGLLHQVKAYEQLAIEAAVHGDYHLALMALTNNPLVPDIGRAKAI
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHH
LDDILRENAVYLPQFKLTTW
HHHHHHCCCEECCCEEEECC
>Mature Secondary Structure 
SRNALKLAVIGGGSSYTPELVEGVLKRLDFLPVKQLHFVDIEAGAEKLQIIRDLAQRMV
CCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DKVGADIEIKAGFDRREAIAGADFVMTQFRVGGLAARANDERIPLKYDVIGQETTGPGGF
HHHCCCEEEECCCCHHHHHCCCHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCCCCHH
AKALRTIPVILDICRDIEELAPNAWMLNFTNPAGLVSEAVSKYSKVKSIGLCNVPVSMQM
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MIAEMMACEPQDLQLEFAGLNHLVWVHQAWLDGQNITQTVLEKVGDGANFSMKNIWEEPW
HHHHHHHCCCCCCEEEECCCCCEEEHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCC
DPEFLKALGAIPCPYHRYFYQTDAMLAEEKKSAQEKGTRAEQVMVTEKALFELYQDPHLA
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
HKPKELEARGGAYYSDASLNLVDAIYNNRNSIHVVNVQNHGAISSLPHDAVIECSAVVGS
CCCCHHHCCCCCEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
WGAKPIAVGELSPKISGLLHQVKAYEQLAIEAAVHGDYHLALMALTNNPLVPDIGRAKAI
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHH
LDDILRENAVYLPQFKLTTW
HHHHHHCCCEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]