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The map label for this gene is gyrA [H]

Identifier: 227081430

GI number: 227081430

Start: 1287646

End: 1290330

Strand: Direct

Name: gyrA [H]

Synonym: VCM66_1213

Alternate gene names: 227081430

Gene position: 1287646-1290330 (Clockwise)

Preceding gene: 227081424

Following gene: 227081431

Centisome position: 44.52

GC content: 51.17

Gene sequence:

>2685_bases
ATGAGCGATCTAGCTAAAGAGATCACGCCCGTAAACATCGAAGACGAGCTACGCAGTTCGTATTTGGACTACGCGATGTC
GGTTATCGTGGGTCGTGCTCTTCCTGATGTGCGTGATGGCCTTAAACCAGTACACCGACGCGTACTGTTCGCGATGAATG
TGCTGGGCAACGACTGGAACAAAGCCTATAAAAAATCTGCCCGTGTGGTAGGTGACGTAATCGGTAAATATCACCCTCAT
GGTGACAGTGCGGTTTACGACACCATCGTGCGTATGGCGCAGCCTTTCTCACTTCGTTACATGTTGGTCGATGGCCAAGG
TAACTTTGGCTCGATCGACGGCGACTCCGCGGCGGCAATGCGTTATACCGAAGTTCGTATGTCGAAAATCGCGCACGAAC
TCTTGGCAGACCTAGACAAAGAAACCGTTGACTACGTTCCGAACTATGACGGCACCGAGCAGATCCCTGCGGTATTGCCG
ACAAAGATCCCGAACCTGCTGATCAACGGCGCATCTGGTATTGCGGTGGGTATGGCAACCAACATTCCTCCGCACAATTT
AGGTGAAGTGATCGATGGCTGTTTAGCCTACATCGATAATGAAGCGATCACCATTGATGAGTTGATGGACTACATTCCTG
GTCCAGATTTCCCAACCGCCGCGCTGATCAGTGGCCGTAAAGGCATCATTGACGCGTACAAAACCGGCCGCGGCAAGGTT
TACATGCGCTCGAAAGCGGAAATCGAAACCGATAAGAACGGCAAAGAAACCATTATCGTCAGCGAAATCCCTTATCAAGT
GAACAAAGCGCGCTTGATCGAAAAAATCGCTGAGTTGGTGAAAGAGAAAAAAGTCGAAGGGATCAGTGCACTGCGTGACG
AGTCTGACAAAGACGGTATGCGCATTGTGATTGAGTGTAAGCGTGATGCGGTTGGCGAAGTGGTTTTGAACAACCTGTAT
GCCAACACTCAGCTGCAAACCACCTTCGGTATCAACATGGTGGCACTCGATAACGGTCAGCCTAAGCTGTTTAACCTGAA
AGAGATGCTGAAGTGTTTCGTCGACCACCGCCGCGAAGTGGTGACTCGTCGTACTATTTTTGAACTGCGTAAAGCGCGTG
AGCGTGCCCACATCCTTGAAGGTTTGGCACTGGCTCTGGCTAACATTGATGAAATCATTGAACTGATCCGCCGTGCCGCC
ACGCCTGCAGAAGCAAAAGAAGGCTTAATTGCCCGTGGTTGGGATCTTGGCAACGTTGCGGCAATGCTTGAGCGTGCAGG
TACGGATGCGGCGCGTCCTGAGTGGCTTGAGCCACAATACGGCATCCGCGATGGCAAGTACTTCCTGACTGAGCAACAAG
CGCAAGCGATTCTGGATCTGCGTCTGCACAAGCTGACTGGTCTAGAGCACGAGAAGATCCTCGATGAGTACAAGCAGCTT
CTCGAAGAAATCGCGGCATTGATGCACATTCTGGCGAGCACAGAGCGCTTGATGGAAGTGATCCGTGAAGAGCTAGAAGC
GATTCGCGCTGGCTTTAGCGATGCGCGCCGCACTGAAATCACGGCAGCAACGCACGACATCGACATGGAAGAGCTGATCG
CTCGTGAAGATGTGGTTGTGACTCTGTCACACGAAGGTTATGTGAAGTATCAATTACTGAGCGACTACGAAGCACAGCGT
CGTGGTGGTAAAGGTAAGAGCGCAACCAAGATGAAAGAAGAGGACTACATTGAGCGTCTTCTGGTTGCCAATACTCACGA
TAACATTCTGCTGTTCTCGACTCGCGGCAAAACTTACCGTATGAAGGTATACCAACTGCCGCTCGCCAGCCGTACTGCGC
GTGGTAAGCCAATCGTGAACCTGCTTCCTCTGGAAGAAAACGAGCGCATCACCGCGATTCTGCCAGTGACTGAGTTTAGT
GAAGATAAATTCATCTTCATGGCAACCGGTGACGGTACTGTGAAGAAAACATCGCTGGATCAGTTTGCCAACGTTCGTGC
GAATGGTTTGATTGCGCTCAACCTGCGTGACGAAGACTCGCTGATTGGTGTGGATATCACCGATGGCGAAAGCGAAATTA
TGCTGTTCTCTAAATTCGGTAAAGTGGTTCGCTTTAAAGAGTCCGAAGAAACCGCGGTTGTGGATGAAAATGGTCAACCT
GTGCTGGATGAAAATGGCCAGCCAGAGATCAAGTTCAAAGGCGTAAGACCAATGGGTCGTACCGCTGCGGGTGTTCGCGG
CATGAAACTGGCTGATGGCGACCAAGTGGTATCGCTCATCGTGCCGAAGACCGAAGGTGACGTACTCACCGTAACTGAAA
ATGGTTACGGCAAACGTACCAGCCTATCTGAATACCCAACCAAAGGTCGTGGAACTCAGGGTGTGGTTTCGATCAAAGTC
TCTGAGCGTAACGGCAGCGTTGTGGGTGCAGTACAAGTGGCCGAGGGTGATGAGTTCATGATGATCACCAATGCCGGTAC
ACTGGTACGTACTCGCGTGGCGGAAGTGAGCCAAGTGGGCCGTAATACTCAAGGCGTGACCCTGATCCGTACTTCGGAAG
GTGAAAGTGTGGTAGGTCTGCAGCGTATCGATGAAATCGAAGAGTCGGAATTGCCAGAAGGTGAGGAAGCACTTGTCGAG
AACGATGCACCTGTTGCACAAGATGATGATGGTGAACAAGAGTAA

Upstream 100 bases:

>100_bases
ATATCCAGTTCTCGACTGACTTGGCGCTCAATCTTGTAGTGAGCTTCGTTTCAGTAAGAATTTGGGTATACCGATCAAAC
TATAGAGGGATAATGGCTCT

Downstream 100 bases:

>100_bases
TTTGCTCTGTCATACCCCTGCCTAATCACCCTTGGGTAGGGAAATTCATAAAGTGCCTAATCAAAGCCGGACAATCGTCC
GGCTTTTTTGATCCTGCCTA

Product: DNA gyrase, subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MSDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWNKAYKKSARVVGDVIGKYHPH
GDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLP
TKIPNLLINGASGIAVGMATNIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKV
YMRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGMRIVIECKRDAVGEVVLNNLY
ANTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREVVTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAA
TPAEAKEGLIARGWDLGNVAAMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQL
LEEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVVTLSHEGYVKYQLLSDYEAQR
RGGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYRMKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFS
EDKFIFMATGDGTVKKTSLDQFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQP
VLDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRTSLSEYPTKGRGTQGVVSIKV
SERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVGRNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVE
NDAPVAQDDDGEQE

Sequences:

>Translated_894_residues
MSDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWNKAYKKSARVVGDVIGKYHPH
GDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLP
TKIPNLLINGASGIAVGMATNIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKV
YMRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGMRIVIECKRDAVGEVVLNNLY
ANTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREVVTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAA
TPAEAKEGLIARGWDLGNVAAMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQL
LEEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVVTLSHEGYVKYQLLSDYEAQR
RGGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYRMKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFS
EDKFIFMATGDGTVKKTSLDQFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQP
VLDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRTSLSEYPTKGRGTQGVVSIKV
SERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVGRNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVE
NDAPVAQDDDGEQE
>Mature_893_residues
SDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWNKAYKKSARVVGDVIGKYHPHG
DSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAMRYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLPT
KIPNLLINGASGIAVGMATNIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKVY
MRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGMRIVIECKRDAVGEVVLNNLYA
NTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREVVTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAAT
PAEAKEGLIARGWDLGNVAAMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQLL
EEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVVTLSHEGYVKYQLLSDYEAQRR
GGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYRMKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFSE
DKFIFMATGDGTVKKTSLDQFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQPV
LDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRTSLSEYPTKGRGTQGVVSIKVS
ERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVGRNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVEN
DAPVAQDDDGEQE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=362, Percent_Identity=26.2430939226519, Blast_Score=84, Evalue=7e-16,
Organism=Homo sapiens, GI19913408, Length=340, Percent_Identity=28.8235294117647, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788562, Length=891, Percent_Identity=74.0740740740741, Blast_Score=1338, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=733, Percent_Identity=36.8349249658936, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI212645845, Length=368, Percent_Identity=25.5434782608696, Blast_Score=77, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535065, Length=286, Percent_Identity=28.6713286713287, Blast_Score=76, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212645657, Length=284, Percent_Identity=28.8732394366197, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=347, Percent_Identity=25.9365994236311, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17136538, Length=354, Percent_Identity=28.5310734463277, Blast_Score=77, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 99027; Mature: 98896

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWN
CCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
KAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHH
RYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLPTKIPNLLINGASGIAVGMAT
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCEEEEEEC
NIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKV
CCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCEE
YMRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGM
EEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIVIECKRDAVGEVVLNNLYANTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREV
EEEEEECCCHHHHHHHHHHHCCCEEEEEECEEEEEECCCCCCEECHHHHHHHHHHHHHHH
VTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAATPAEAKEGLIARGWDLGNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHCCCEECCCCHHHHH
AMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQL
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCCCHHHHHCCCCEEE
TLSHEGYVKYQLLSDYEAQRRGGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYR
EEECCCEEEEEEHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHCCCCCCEEEEEECCCEEE
MKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFSEDKFIFMATGDGTVKKTSLD
EEEEECCCCCCCCCCCCCEEEECCCCCCCEEEEEECEECCCCCEEEEEECCCCEEECCHH
QFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQP
HHHCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCEEECCCCCC
VLDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRT
CCCCCCCCCEEEECCCCCCCHHHCCCCCEECCCCEEEEEEEECCCCCEEEEECCCCCCCC
SLSEYPTKGRGTQGVVSIKVSERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVG
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHC
RNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVENDAPVAQDDDGEQE
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDLAKEITPVNIEDELRSSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWN
CCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHH
KAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYMLVDGQGNFGSIDGDSAAAM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHH
RYTEVRMSKIAHELLADLDKETVDYVPNYDGTEQIPAVLPTKIPNLLINGASGIAVGMAT
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHEEECCCCEEEEEEC
NIPPHNLGEVIDGCLAYIDNEAITIDELMDYIPGPDFPTAALISGRKGIIDAYKTGRGKV
CCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCCCHHHHHCCCCCCHHHHCCCCCEE
YMRSKAEIETDKNGKETIIVSEIPYQVNKARLIEKIAELVKEKKVEGISALRDESDKDGM
EEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RIVIECKRDAVGEVVLNNLYANTQLQTTFGINMVALDNGQPKLFNLKEMLKCFVDHRREV
EEEEEECCCHHHHHHHHHHHCCCEEEEEECEEEEEECCCCCCEECHHHHHHHHHHHHHHH
VTRRTIFELRKARERAHILEGLALALANIDEIIELIRRAATPAEAKEGLIARGWDLGNVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHCCCEECCCCHHHHH
AMLERAGTDAARPEWLEPQYGIRDGKYFLTEQQAQAILDLRLHKLTGLEHEKILDEYKQL
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEEIAALMHILASTERLMEVIREELEAIRAGFSDARRTEITAATHDIDMEELIAREDVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCCCHHHHHCCCCEEE
TLSHEGYVKYQLLSDYEAQRRGGKGKSATKMKEEDYIERLLVANTHDNILLFSTRGKTYR
EEECCCEEEEEEHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHCCCCCCEEEEEECCCEEE
MKVYQLPLASRTARGKPIVNLLPLEENERITAILPVTEFSEDKFIFMATGDGTVKKTSLD
EEEEECCCCCCCCCCCCCEEEECCCCCCCEEEEEECEECCCCCEEEEEECCCCEEECCHH
QFANVRANGLIALNLRDEDSLIGVDITDGESEIMLFSKFGKVVRFKESEETAVVDENGQP
HHHCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCEEECCCCCC
VLDENGQPEIKFKGVRPMGRTAAGVRGMKLADGDQVVSLIVPKTEGDVLTVTENGYGKRT
CCCCCCCCCEEEECCCCCCCHHHCCCCCEECCCCEEEEEEEECCCCCEEEEECCCCCCCC
SLSEYPTKGRGTQGVVSIKVSERNGSVVGAVQVAEGDEFMMITNAGTLVRTRVAEVSQVG
CCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHC
RNTQGVTLIRTSEGESVVGLQRIDEIEESELPEGEEALVENDAPVAQDDDGEQE
CCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8849216 [H]