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The map label for this gene is nrdB [H]

Identifier: 227081427

GI number: 227081427

Start: 1282594

End: 1283763

Strand: Reverse

Name: nrdB [H]

Synonym: VCM66_1210

Alternate gene names: 227081427

Gene position: 1283763-1282594 (Counterclockwise)

Preceding gene: 227081428

Following gene: 227081426

Centisome position: 44.38

GC content: 46.24

Gene sequence:

>1170_bases
TTGCTTAGGCAAGAGGCCAAACGGATGAAGGCAACAATGGCTTACAGTACTTTTACCCAAAAGAAGAATGACCAACTTAA
AGAACCTATGTTCCTTGGTCAGCCTGTCAACGTCGCACGTTACGACCAACAAAAATATGAGATTTTTGAGAAGCTGATTG
AAAAGCAACTCTCCTTCTTCTGGCGTCCAGAAGAAGTGGATGTATCCAGCGATCGAATTGACTACGGCAAATTGCCGGAT
CATGAAAAGCATATTTTCATTTCTAACCTGAAATACCAAACTCTGCTCGACTCCATTCAAGGCCGTAGCCCGAACGTCGC
ACTGCTGCCACTGGTTTCACTGCCAGAGCTGGAAACGTGGATTGAAACTTGGTCTTTCTCTGAAACCATTCACTCACGCT
CTTACACTCACATCATTCGTAATATCGTGAATGATCCTGCAGTGGTGTTTGACGACATCGTTGAAAACGAGCACATCATC
AAGCGTGCGAAAGACATCGCCCACTACTACGATGATTTGATTGAGCTGACTAATGACTATCACCGCTATGGTGAAGGCCA
ACATCAGATCAACGGTGAAACCATCACGGTATCGCTGAAAGAGCTGAAGAAGAAGCTGTATCTCTGCTTGATGTCAGTCA
ATGCACTGGAGGCGATTCGCTTCTATGTAAGCTTTGCCTGCTCTTTCGCGTTTGCAGAACGTGAACTGATGGAAGGTAAC
GCGAAAATCATCAAACTGATTGCACGTGACGAAGCCTTGCACCTGACGGGTACTCAACACATGTTGAATCTACTGCGCAA
CGGCATGGATGATTTTACCTTCCTGCAAGTGGCAGAAGAGTGTAAGCAAGAAAGCTTCGATTTGTTCAAAGAAGCCGCTG
AACAAGAGAAAGAGTGGGCAGAATACCTGTTCAAAGACGGTTCGATGATCGGCCTTAATAAAGATATTCTGTGTCAGTAC
GTCGAGTACATTACCAATATCCGTATGCAAGCCGTCGGTTTAGAGCCGGCATACCCAGCCGCGACGAATAACCCGATCCC
ATGGATCAACGCATGGCTGTCTTCTGATAACGTGCAAGTGGCACCTCAAGAGGCTGAGATCAGTTCTTACTTAGTGGGTC
AAATCGACAACGAGGTAAGCGCCGATGATTTCGAGGGCTTTGAACTGTAA

Upstream 100 bases:

>100_bases
TGCAGGAAGACGATTGTGCCAGCGGCGCTTGTAAGATCTAAGCGCCTGCTTCTAAAGCACAACACGAGAAAATAGTTTAA
CTCTTTGATTTGCATGCCTC

Downstream 100 bases:

>100_bases
TGGCTGCAATCAAAATCAACAAGCTGCTGACCATTGAATCTAACTCGAGCCATACTCTGCTTGAAAGTATGGAACAAGCA
GGGTTAGAGCCAGAATACAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Protein B2; Protein R2; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MLRQEAKRMKATMAYSTFTQKKNDQLKEPMFLGQPVNVARYDQQKYEIFEKLIEKQLSFFWRPEEVDVSSDRIDYGKLPD
HEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPAVVFDDIVENEHII
KRAKDIAHYYDDLIELTNDYHRYGEGQHQINGETITVSLKELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGN
AKIIKLIARDEALHLTGTQHMLNLLRNGMDDFTFLQVAEECKQESFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQY
VEYITNIRMQAVGLEPAYPAATNNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVSADDFEGFEL

Sequences:

>Translated_389_residues
MLRQEAKRMKATMAYSTFTQKKNDQLKEPMFLGQPVNVARYDQQKYEIFEKLIEKQLSFFWRPEEVDVSSDRIDYGKLPD
HEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPAVVFDDIVENEHII
KRAKDIAHYYDDLIELTNDYHRYGEGQHQINGETITVSLKELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGN
AKIIKLIARDEALHLTGTQHMLNLLRNGMDDFTFLQVAEECKQESFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQY
VEYITNIRMQAVGLEPAYPAATNNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVSADDFEGFEL
>Mature_389_residues
MLRQEAKRMKATMAYSTFTQKKNDQLKEPMFLGQPVNVARYDQQKYEIFEKLIEKQLSFFWRPEEVDVSSDRIDYGKLPD
HEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETWIETWSFSETIHSRSYTHIIRNIVNDPAVVFDDIVENEHII
KRAKDIAHYYDDLIELTNDYHRYGEGQHQINGETITVSLKELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGN
AKIIKLIARDEALHLTGTQHMLNLLRNGMDDFTFLQVAEECKQESFDLFKEAAEQEKEWAEYLFKDGSMIGLNKDILCQY
VEYITNIRMQAVGLEPAYPAATNNPIPWINAWLSSDNVQVAPQEAEISSYLVGQIDNEVSADDFEGFEL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2 contains the tyrosyl radical required for catalysis [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1788567, Length=377, Percent_Identity=81.1671087533157, Blast_Score=651, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 45084; Mature: 45084

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00368 RIBORED_SMALL ; PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQEAKRMKATMAYSTFTQKKNDQLKEPMFLGQPVNVARYDQQKYEIFEKLIEKQLSFF
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
WRPEEVDVSSDRIDYGKLPDHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETW
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
IETWSFSETIHSRSYTHIIRNIVNDPAVVFDDIVENEHIIKRAKDIAHYYDDLIELTNDY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HRYGEGQHQINGETITVSLKELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGN
HHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKIIKLIARDEALHLTGTQHMLNLLRNGMDDFTFLQVAEECKQESFDLFKEAAEQEKEWA
HHHEEEEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLFKDGSMIGLNKDILCQYVEYITNIRMQAVGLEPAYPAATNNPIPWINAWLSSDNVQV
HHHHCCCCEECCCHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCHHHHEECCCCCEE
APQEAEISSYLVGQIDNEVSADDFEGFEL
CCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRQEAKRMKATMAYSTFTQKKNDQLKEPMFLGQPVNVARYDQQKYEIFEKLIEKQLSFF
CCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
WRPEEVDVSSDRIDYGKLPDHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSLPELETW
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
IETWSFSETIHSRSYTHIIRNIVNDPAVVFDDIVENEHIIKRAKDIAHYYDDLIELTNDY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
HRYGEGQHQINGETITVSLKELKKKLYLCLMSVNALEAIRFYVSFACSFAFAERELMEGN
HHHCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKIIKLIARDEALHLTGTQHMLNLLRNGMDDFTFLQVAEECKQESFDLFKEAAEQEKEWA
HHHEEEEECCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYLFKDGSMIGLNKDILCQYVEYITNIRMQAVGLEPAYPAATNNPIPWINAWLSSDNVQV
HHHHCCCCEECCCHHHHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCHHHHEECCCCCEE
APQEAEISSYLVGQIDNEVSADDFEGFEL
CCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]