Click here to switch to the map view.

The map label for this gene is polB [H]

Identifier: 227081384

GI number: 227081384

Start: 1245275

End: 1247638

Strand: Reverse

Name: polB [H]

Synonym: VCM66_1167

Alternate gene names: 227081384

Gene position: 1247638-1245275 (Counterclockwise)

Preceding gene: 227081389

Following gene: 227081381

Centisome position: 43.13

GC content: 48.18

Gene sequence:

>2364_bases
TTGAAGATTGAACAAGGGTTTGTATTAACGCGTCACGCTCGTGATGTGGCTGGACAAACCCAAATTGAACTTTGGCTCGC
GACACCTTCAGGCCCAACGCAACTTACCATACGCGGTGAGCGTCCGGTTTTTTTTATTGAACAATCGGCTAGCCAAGAAG
TGATGCGAATTGCCGCAGAATTATCAATAACGCCCTCATTAGCGCCTCTGTCATTACGTAGCTTTGCAGGCCAACCCCTC
GCCGCTTGTTACTGCAATACGATTCGCGATAGCCAAATCTTAGCCGAGAAACTCGCTCAAGCGGACATGTTGATCTTAGA
GGCGGATATTCGTTTGGCTGACCGTTTTCTGATGGAACGCTTTATCCAAGGCAGCATCGAATTTACTGGTCAGATCACCG
ATTTCGGCCATTATCGCCAAGTTCAGCAAGCCAAATGTCGCCAAGGTGATTACCTACCGACGCTGAATATGGTGTCGCTA
GACATCGAATGCTCCGAAAAAGGGCTACTCTACTCGATTGGTTTGGATAGCCCGATGGACAGCCGAGTGATTATGATAGG
TCAACCAGAACCCGCGGAAACGCCGATTCAATGGGTCGAGGATGAATACCAGTTACTCAAAGCGCTCATTGCATGGTTTG
AGCAGTTTGATCCTGATGTGATTATTGGTTGGAGTGTAGTGGATTTCGATTTCCGGCTGCTGCACAAACGCGCGGAGTTC
CACAAACTGAAACTCACCATTGGCCGAGCTCAGCAACCGAGCTTTTTCCGTACGGCGAGCCAAACGCAGCAGGGTTTTAT
CAGTATTCCTGGGCGTGTGGTGCTCGATGGTATCGATACGTTAAAAACCGCGACTTATCATTTTCGTTCATGGTCTTTGG
AGTCGGTCTCGCAAGAGCTGCTCGGCGAAGGGAAAGCGATTCATAACGTGCATGATCGGATGGATGAAATTAACCAGATG
TTTCGCCACGATAAGCCCTCACTCGCTCGTTACAACCTGCAAGACTGCGTATTAGTGAATAAGATTTTTGCGGCCACTCA
TCTGCTCGATTTCGCCATTCAGCGTTCACGTTTAACGGGTGTCGAGTTAGATCGCATTGGTGGCTCCGTGGCCGCATTCA
CCAATCTCTATCTACCACAACTGCACCGCGCAGGGTATGTCGCTCCCAATTTGCAGCCAGAAAACTGGGTTGCCAGCCCG
GGCGGTTATGTGATGGATTCGATTCCAGGGCTGTATGACTCGGTACTCGTGCTCGACTTTAAAAGCCTCTACCCTTCGAT
TATTCGCTCGTTTTTGATTGATCCACTTGGGCTGATTGAAGGATTAAAACTGCCAATAGGCAAACAGGCCGATCACGCGG
TTCCGGGCTTTCGCGGCGGTCAATTTCATCGCACCAAACACTTTTTACCTGAGATGATCGAAAAACTATGGGCAGCGCGC
GATGAAGCCAAACGCAACCAAGAAAAGGCTTTTTCACAAGCAATCAAAATCATCATGAACTCATTTTATGGTGTGCTCGG
CTCATCAGGCTGCCGCTTTTTCGATGCGCGATTGGCATCGAGCATTACCATGCGCGGCCATGAAATCATGATCCAAACAA
AACGGTTGATTGAAGCGCGCGGTTATCAAGTGATCTATGGCGATACGGATTCAACGTTTGTCGCACTCGGTGGGCAATAC
TCTCAGCAAGAAGCGGATAACATTGGTCACGCTTTGGTCCGAGAAATCAACCAATGGTGGACAGAGCATTTAAAGCAAGA
ATATGCCCTTACTTCGATTCTTGAACTCGAGTACGAAACCCACTATCGCCGTTTTTTAATGCCGACCATTCGCGGTTCGG
AAACGGGCTCCAAAAAACGCTATGCGGGTTTAAAAGGGGATGGTGACAATGAGCAACTGATTTTTAAAGGTTTGGAAAGC
GCACGCACCGACTGGACTCCACTCGCGCAACGGTTTCAACATCAGCTGTATCAATTGATTTTCCACGGTCAAGACCCAGA
ATCCTATATTCGCACTATCGTAGAACAGACATTGGCAGGTCAATTGGATGATCAATTGGTATATCAAAAGCGTTTACGCC
GACGGTTACATGAGTACCAAAAGAATGTGCCGCCGCAAGTACGCGCAGCCAGAATGGCGGATGACATTAACGCCAAACTT
GGGCGACCGTTACAATACCAATACCGCGGAACGATTGAATATGTGATTACAGTCAATGGTCCTGAGCCGAAAGAGTATTC
CAAAAGCCCTATCGATTATCAGCACTACATCGATAAACAGCTTAAACCGGTCGCAGATGCGATTTTGCCTTTTATTGGCA
AACAATTTGATGAGCTTATTGCGCCTCAACTGGGACTGTTTTAG

Upstream 100 bases:

>100_bases
CCACTCCTGCTTGTGATGCCACAATGCGATGTAATCAGCTTCACTATCAGTATAATGTGCCAGCGCAAAAATGTATGAGT
GTTGAAAAAGGTGGGCCAGA

Downstream 100 bases:

>100_bases
AAGCGTTTCGACGCTGTACAAACTCGACGAAATCACCACGATTCAGAGATTTACTGTAAAACCAACCTTGTACAGAGAAT
GGGTATTCATCTCCCATCGC

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAELSITPSLAPLSLRSFAGQPL
AACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMERFIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSL
DIECSEKGLLYSIGLDSPMDSRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKAIHNVHDRMDEINQM
FRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTGVELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASP
GGYVMDSIPGLYDSVLVLDFKSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEARGYQVIYGDTDSTFVALGGQY
SQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYETHYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLES
ARTDWTPLAQRFQHQLYQLIFHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELIAPQLGLF

Sequences:

>Translated_787_residues
MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAELSITPSLAPLSLRSFAGQPL
AACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMERFIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSL
DIECSEKGLLYSIGLDSPMDSRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKAIHNVHDRMDEINQM
FRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTGVELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASP
GGYVMDSIPGLYDSVLVLDFKSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEARGYQVIYGDTDSTFVALGGQY
SQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYETHYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLES
ARTDWTPLAQRFQHQLYQLIFHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELIAPQLGLF
>Mature_787_residues
MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAELSITPSLAPLSLRSFAGQPL
AACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMERFIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSL
DIECSEKGLLYSIGLDSPMDSRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQELLGEGKAIHNVHDRMDEINQM
FRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTGVELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASP
GGYVMDSIPGLYDSVLVLDFKSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEARGYQVIYGDTDSTFVALGGQY
SQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYETHYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLES
ARTDWTPLAQRFQHQLYQLIFHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELIAPQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=703, Percent_Identity=28.4495021337127, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI106507301, Length=680, Percent_Identity=24.4117647058824, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI1786246, Length=794, Percent_Identity=52.7707808564232, Blast_Score=819, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=703, Percent_Identity=26.7425320056899, Blast_Score=156, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32565317, Length=657, Percent_Identity=26.4840182648402, Blast_Score=132, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320101, Length=714, Percent_Identity=25.2100840336134, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324227, Length=574, Percent_Identity=24.9128919860627, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325090, Length=720, Percent_Identity=22.2222222222222, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24664937, Length=704, Percent_Identity=24.4318181818182, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24648780, Length=652, Percent_Identity=23.7730061349693, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 89629; Mature: 89629

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAE
CCCCCCCEEEEHHHHCCCCEEEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHH
LSITPSLAPLSLRSFAGQPLAACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMER
HCCCCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHH
FIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSLDIECSEKGLLYSIGLDSPMD
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCC
SRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHH
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQEL
HEEEEEECCCCCCHHHHHHHHHCCCEEECCCEEEECCHHHHHHHHHHHCCCCHHHHHHHH
LGEGKAIHNVHDRMDEINQMFRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTG
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASPGGYVMDSIPGLYDSVLVLDF
CCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
KSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
HHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHC
GYQVIYGDTDSTFVALGGQYSQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLESARTDWTPLAQRFQHQLYQLI
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELI
CCCEEEEECCEEEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APQLGLF
CCCCCCC
>Mature Secondary Structure
MKIEQGFVLTRHARDVAGQTQIELWLATPSGPTQLTIRGERPVFFIEQSASQEVMRIAAE
CCCCCCCEEEEHHHHCCCCEEEEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHH
LSITPSLAPLSLRSFAGQPLAACYCNTIRDSQILAEKLAQADMLILEADIRLADRFLMER
HCCCCCCCCHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHH
FIQGSIEFTGQITDFGHYRQVQQAKCRQGDYLPTLNMVSLDIECSEKGLLYSIGLDSPMD
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCC
SRVIMIGQPEPAETPIQWVEDEYQLLKALIAWFEQFDPDVIIGWSVVDFDFRLLHKRAEF
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHH
HKLKLTIGRAQQPSFFRTASQTQQGFISIPGRVVLDGIDTLKTATYHFRSWSLESVSQEL
HEEEEEECCCCCCHHHHHHHHHCCCEEECCCEEEECCHHHHHHHHHHHCCCCHHHHHHHH
LGEGKAIHNVHDRMDEINQMFRHDKPSLARYNLQDCVLVNKIFAATHLLDFAIQRSRLTG
HHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VELDRIGGSVAAFTNLYLPQLHRAGYVAPNLQPENWVASPGGYVMDSIPGLYDSVLVLDF
CCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
KSLYPSIIRSFLIDPLGLIEGLKLPIGKQADHAVPGFRGGQFHRTKHFLPEMIEKLWAAR
HHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DEAKRNQEKAFSQAIKIIMNSFYGVLGSSGCRFFDARLASSITMRGHEIMIQTKRLIEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHC
GYQVIYGDTDSTFVALGGQYSQQEADNIGHALVREINQWWTEHLKQEYALTSILELEYET
CCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYRRFLMPTIRGSETGSKKRYAGLKGDGDNEQLIFKGLESARTDWTPLAQRFQHQLYQLI
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
FHGQDPESYIRTIVEQTLAGQLDDQLVYQKRLRRRLHEYQKNVPPQVRAARMADDINAKL
HCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GRPLQYQYRGTIEYVITVNGPEPKEYSKSPIDYQHYIDKQLKPVADAILPFIGKQFDELI
CCCEEEEECCEEEEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APQLGLF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]