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The map label for this gene is hutC [H]

Identifier: 227081378

GI number: 227081378

Start: 1239305

End: 1240015

Strand: Reverse

Name: hutC [H]

Synonym: VCM66_1161

Alternate gene names: 227081378

Gene position: 1240015-1239305 (Counterclockwise)

Preceding gene: 227081379

Following gene: 227081377

Centisome position: 42.87

GC content: 49.93

Gene sequence:

>711_bases
TTGTCTCCACGCTATCTGCAAATCAAACAATTTATGCTCGATAACATCGAGTCGGGTCGTTGGCCAGTCGGTTATCGTAC
CCCCACTGAATTGGAGTTAACCGAGCAATTTGGTGTCAGCCGCATGACGGTCAACAAAGCGCTGCGCGATCTGGTCAGTG
AAGGGAAATTACAGCGCCGCCCACGTTTAGGCACCTTTGTTTGTGCGGCAGAAGAGAAAGCCGAATCGCCTTTGCTGGAT
ATTCGCAACATCGCCGATGAAATCCTCAGTCGCGGTAAGCAGTATCACAATAAAGTGGTTGAACAGCGTGCGCTAAGCGC
CGATGAAGCTGTCGCCATCAAGCTCGGTGTAATGGTGGGAAGCCCAGTGTTTTACAGTGAAATCATTCACTACGCTAACG
ACATCCCGATGCAACTGGAAGTGCGTTGGGTGAATAGCCGTTCGGTGCCACACTATTTGGATCAAGATTTTACCCAAATT
ACGCCCAACCAATATTTGTCGCAAAACTGTCCACTGAGCGCGATTGAACATACTGTAGAAGCCATTGTGGCCGATGCGCA
TGTCAAACAAGCCCTTGCACTTGCGGCTAATGAACCTTGCTTGCTACTCAATCGCCGTACTTGGAGTGGTGACAAGCTGG
TGAGCTCAGCCCTGCTCTATCATCCGGGTTCGCGCTATAAACTCAGCTCGAAAATTCTGTTCAGCCAATAG

Upstream 100 bases:

>100_bases
AAATCTGCGCAATGTGGCAACTTACACACAAAAAGTTGCAAAACTTCGATTTCAAAGAAATGATTTGTCTATGCAAATGA
TTTTTTGAAAGAGTTGAAAC

Downstream 100 bases:

>100_bases
CCCCACATGATCACATTTTCGCAACATTCAAGTTGCGAACTTTCACGCTTTCCACTATTTATTTGTATATACATTTGAAA
TATCGATATCTGGATGGAAA

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSPRYLQIKQFMLDNIESGRWPVGYRTPTELELTEQFGVSRMTVNKALRDLVSEGKLQRRPRLGTFVCAAEEKAESPLLD
IRNIADEILSRGKQYHNKVVEQRALSADEAVAIKLGVMVGSPVFYSEIIHYANDIPMQLEVRWVNSRSVPHYLDQDFTQI
TPNQYLSQNCPLSAIEHTVEAIVADAHVKQALALAANEPCLLLNRRTWSGDKLVSSALLYHPGSRYKLSSKILFSQ

Sequences:

>Translated_236_residues
MSPRYLQIKQFMLDNIESGRWPVGYRTPTELELTEQFGVSRMTVNKALRDLVSEGKLQRRPRLGTFVCAAEEKAESPLLD
IRNIADEILSRGKQYHNKVVEQRALSADEAVAIKLGVMVGSPVFYSEIIHYANDIPMQLEVRWVNSRSVPHYLDQDFTQI
TPNQYLSQNCPLSAIEHTVEAIVADAHVKQALALAANEPCLLLNRRTWSGDKLVSSALLYHPGSRYKLSSKILFSQ
>Mature_235_residues
SPRYLQIKQFMLDNIESGRWPVGYRTPTELELTEQFGVSRMTVNKALRDLVSEGKLQRRPRLGTFVCAAEEKAESPLLDI
RNIADEILSRGKQYHNKVVEQRALSADEAVAIKLGVMVGSPVFYSEIIHYANDIPMQLEVRWVNSRSVPHYLDQDFTQIT
PNQYLSQNCPLSAIEHTVEAIVADAHVKQALALAANEPCLLLNRRTWSGDKLVSSALLYHPGSRYKLSSKILFSQ

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788418, Length=207, Percent_Identity=28.0193236714976, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786950, Length=142, Percent_Identity=29.5774647887324, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26698; Mature: 26567

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRYLQIKQFMLDNIESGRWPVGYRTPTELELTEQFGVSRMTVNKALRDLVSEGKLQRR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHCCHHHCC
PRLGTFVCAAEEKAESPLLDIRNIADEILSRGKQYHNKVVEQRALSADEAVAIKLGVMVG
CCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHCC
SPVFYSEIIHYANDIPMQLEVRWVNSRSVPHYLDQDFTQITPNQYLSQNCPLSAIEHTVE
CHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHCCHHHHCCCHHHHHCCCCHHHHHHHHH
AIVADAHVKQALALAANEPCLLLNRRTWSGDKLVSSALLYHPGSRYKLSSKILFSQ
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SPRYLQIKQFMLDNIESGRWPVGYRTPTELELTEQFGVSRMTVNKALRDLVSEGKLQRR
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHCCHHHCC
PRLGTFVCAAEEKAESPLLDIRNIADEILSRGKQYHNKVVEQRALSADEAVAIKLGVMVG
CCCCEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHCC
SPVFYSEIIHYANDIPMQLEVRWVNSRSVPHYLDQDFTQITPNQYLSQNCPLSAIEHTVE
CHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHCCHHHHCCCHHHHHCCCCHHHHHHHHH
AIVADAHVKQALALAANEPCLLLNRRTWSGDKLVSSALLYHPGSRYKLSSKILFSQ
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]