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The map label for this gene is dhaT [H]

Identifier: 227081320

GI number: 227081320

Start: 1175235

End: 1176503

Strand: Direct

Name: dhaT [H]

Synonym: VCM66_1103

Alternate gene names: 227081320

Gene position: 1175235-1176503 (Clockwise)

Preceding gene: 227081317

Following gene: 227081326

Centisome position: 40.63

GC content: 51.69

Gene sequence:

>1269_bases
GTGAAAGGCCTTGGAGGGAGCATGTTCCAGTTTATGACCGCGACACGGATCATCTTTGGTGAGGGGTCGCTAGAGAGCTC
CCTTTCGATAATCAGCCAGTACGGATACAGCGTGCTACTGGTCTCGGGGAAAAACGCTGAGCGCTATGCTCCTCTAACGG
CCTATCTACAACAGCAGAACATGCGCTACCAACATGTTGTAGTTTCTGGCGAGCCCAACATCACTATGGTTGAAGAAGCC
GCGATGCTTGGTCGTCGCTTTAAACCTGACATGGTGATTGCGATAGGCGGCGGTAGTGTGTTGGATATGGGGAAAGCGGT
CGCGGCGATTATCCCCAATCAAGGCAACGTGTATGACTATGTTGAGGTGGTTGGGCGCAGTGTGCCACTCAAAGCCAAAC
CACTGCCATTTATCGCGATTCCGACTACTGCTAGCTCGGGTTCTGAAGTGACGCGTAATGCGGTGCTGAAATCGGCGCAG
GACAGAGTCAAAATTAGCTTGCGCAGTCCTGAGATGTTAGCGGACGTTGCGATCATTGATCCCACTTTGACTTACGGTAC
AGATCCTGTGACGTCTGGTCGAGGAGCCATGGATGCGTTTACCCATCTTATGGAAGCTTACGTGTGTGGAGAGCCCAATC
CTCTAACCGATATGATTTGTGAGGAAGGGTTACGGCGTTTAAGCCGAGCGATGATCGCGGGCTGCTTGCGCGATGATAAA
AAAGCACGCAGTGATCTCTCCTTCGCTGCCATGCTGGGGGGAATGGCGACAACCAATGCCAAATTAGGGGCCGCCCATGG
ACTGGCTTCTGCACTGGGCGGCAAATTGGATGCGCCACACAGTGTGATTACCGCGCGACTTGCGCCGATTGTGATGCGAG
AAAATATTGATGTGGCTAAAGAGGCTGGGCGCAGTGATATTCTACAGCGCTATAAGATCGTTTCGCGCATTCTGACTGGC
CGTAAAAATGCCGGTGTGGAAGATGGCATCTTATGGGTCAATATGATGCTAGAAAAATTATCGATTCCTAATTTGACCGA
TTTTGGTGTCTGCGCGACCTCTTTTGAACGCGTGGCCGCAGATGCACTGAAATCAACAGCGATTAAAGGTAACCCGATCC
CTTTGACCATGGAGCGCTTGACTCACATTCTGCATCAAGTCTGCTCTTGTCAACCACTTGCGGCGATAGAGCCTCGAGCA
TCACAAGGTGAGGTGGAGATTCGCCATTTGCTAAAAGAGGAAGGAAGCCGCTTGAGCGGGCAACATTGA

Upstream 100 bases:

>100_bases
TGTGCAAGAACTAGCAGATTTTTTGTGGTTAATTTCGGCAAGTTGCACTGGGTCAAACAAATTTCCCCCATTTGGATGCA
AGCTTAGCACTATCTGATGT

Downstream 100 bases:

>100_bases
GCCTATTCCTCCTTATTGGAAACCATCATTATTCTGACTCAAAATGAAAACGGAGCCACTGGGCTCCGTTATTCTGTCTT
TGTGTGTGGCCTAGTAAGGC

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKGLGGSMFQFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTAYLQQQNMRYQHVVVSGEPNITMVEEA
AMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDYVEVVGRSVPLKAKPLPFIAIPTTASSGSEVTRNAVLKSAQ
DRVKISLRSPEMLADVAIIDPTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK
KARSDLSFAAMLGGMATTNAKLGAAHGLASALGGKLDAPHSVITARLAPIVMRENIDVAKEAGRSDILQRYKIVSRILTG
RKNAGVEDGILWVNMMLEKLSIPNLTDFGVCATSFERVAADALKSTAIKGNPIPLTMERLTHILHQVCSCQPLAAIEPRA
SQGEVEIRHLLKEEGSRLSGQH

Sequences:

>Translated_422_residues
MKGLGGSMFQFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTAYLQQQNMRYQHVVVSGEPNITMVEEA
AMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDYVEVVGRSVPLKAKPLPFIAIPTTASSGSEVTRNAVLKSAQ
DRVKISLRSPEMLADVAIIDPTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK
KARSDLSFAAMLGGMATTNAKLGAAHGLASALGGKLDAPHSVITARLAPIVMRENIDVAKEAGRSDILQRYKIVSRILTG
RKNAGVEDGILWVNMMLEKLSIPNLTDFGVCATSFERVAADALKSTAIKGNPIPLTMERLTHILHQVCSCQPLAAIEPRA
SQGEVEIRHLLKEEGSRLSGQH
>Mature_422_residues
MKGLGGSMFQFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTAYLQQQNMRYQHVVVSGEPNITMVEEA
AMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDYVEVVGRSVPLKAKPLPFIAIPTTASSGSEVTRNAVLKSAQ
DRVKISLRSPEMLADVAIIDPTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK
KARSDLSFAAMLGGMATTNAKLGAAHGLASALGGKLDAPHSVITARLAPIVMRENIDVAKEAGRSDILQRYKIVSRILTG
RKNAGVEDGILWVNMMLEKLSIPNLTDFGVCATSFERVAADALKSTAIKGNPIPLTMERLTHILHQVCSCQPLAAIEPRA
SQGEVEIRHLLKEEGSRLSGQH

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994951, Length=382, Percent_Identity=31.151832460733, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789163, Length=388, Percent_Identity=30.9278350515464, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082107, Length=316, Percent_Identity=33.5443037974684, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787493, Length=345, Percent_Identity=27.536231884058, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789386, Length=361, Percent_Identity=25.207756232687, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17537053, Length=287, Percent_Identity=25.4355400696864, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321181, Length=378, Percent_Identity=31.2169312169312, Blast_Score=166, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24657991, Length=293, Percent_Identity=25.5972696245734, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 45343; Mature: 45343

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLGGSMFQFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTAYLQQQN
CCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
MRYQHVVVSGEPNITMVEEAAMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDY
CCEEEEEEECCCCEEEHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHEEEECCCCCHHHH
VEVVGRSVPLKAKPLPFIAIPTTASSGSEVTRNAVLKSAQDRVKISLRSPEMLADVAIID
HHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHEEEEC
PTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KARSDLSFAAMLGGMATTNAKLGAAHGLASALGGKLDAPHSVITARLAPIVMRENIDVAK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHH
EAGRSDILQRYKIVSRILTGRKNAGVEDGILWVNMMLEKLSIPNLTDFGVCATSFERVAA
HCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DALKSTAIKGNPIPLTMERLTHILHQVCSCQPLAAIEPRASQGEVEIRHLLKEEGSRLSG
HHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
QH
CC
>Mature Secondary Structure
MKGLGGSMFQFMTATRIIFGEGSLESSLSIISQYGYSVLLVSGKNAERYAPLTAYLQQQN
CCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
MRYQHVVVSGEPNITMVEEAAMLGRRFKPDMVIAIGGGSVLDMGKAVAAIIPNQGNVYDY
CCEEEEEEECCCCEEEHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHEEEECCCCCHHHH
VEVVGRSVPLKAKPLPFIAIPTTASSGSEVTRNAVLKSAQDRVKISLRSPEMLADVAIID
HHHHCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHEEEEC
PTLTYGTDPVTSGRGAMDAFTHLMEAYVCGEPNPLTDMICEEGLRRLSRAMIAGCLRDDK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KARSDLSFAAMLGGMATTNAKLGAAHGLASALGGKLDAPHSVITARLAPIVMRENIDVAK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHH
EAGRSDILQRYKIVSRILTGRKNAGVEDGILWVNMMLEKLSIPNLTDFGVCATSFERVAA
HCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DALKSTAIKGNPIPLTMERLTHILHQVCSCQPLAAIEPRASQGEVEIRHLLKEEGSRLSG
HHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
QH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]