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The map label for this gene is purB [H]

Identifier: 227081299

GI number: 227081299

Start: 1150805

End: 1152175

Strand: Reverse

Name: purB [H]

Synonym: VCM66_1082

Alternate gene names: 227081299

Gene position: 1152175-1150805 (Counterclockwise)

Preceding gene: 227081300

Following gene: 227081289

Centisome position: 39.83

GC content: 49.53

Gene sequence:

>1371_bases
ATGGAACTGTCAGCATTGACTGCTGTTTCACCGGTAGATGGCCGCTACGGAAGTAAAACGATTGCGTTACGCAGTATCTT
CAGTGAGTTTGGTCTCCTAAAGTACCGCACTATCGTTGAAATTCGCTGGCTACAAAAGCTAGCCGCCACTGCTGAGATCG
CAGAAGTGCCTGCATTCAGTGCCGAAGCGAATCAATTCCTTGATGCGATTGCCGCCAACTTCAATGAGGCGGATGCACTC
CGTATCAAAGAGATTGAACGCACCACCAACCACGATGTAAAAGCCGTGGAATACTTCCTGAAAGAGAAAGTAGCGGCCAT
GCCTGAGCTGCATGCCGTCAACGAGTTCATTCACTTTGCTTGTACTTCCGAAGACATCAACAACACTTCACATGCGTTGA
TGCTCAAAGAAGCGCGTGACACGGTGATCCTGCCTGAAATTCGCAACGTGATTGACGCAATTCGCAAACTGGCCGAAGAG
TACCGCGATATTCCGCTGCTTTCTCGTACTCACGGTCAGCCAGCCTCTCCATCGACCATGGGTAAAGAGATGGCGAACGT
GGCCTACCGCATGGAGCGCCAATACAAGCAGATCGCTAACGTAGAAATCCTAGCCAAAATCAACGGTGCGGTGGGTAACT
ACAACGCGCACCTTTCTGCTTACCCAACGGTAGATTGGCATAAATTCAGCGAAGAGTTCATCACTGAATCTTTGGGCGTA
GATTGGAACCCGTACACCACGCAAATCGAGCCGCATGATTACATTGCTGAGCTGTTTGAAGCGGTGGCACGTTTTAACAC
CATCTTGATCGATTTTGATCGTGATGTTTGGGGTTACATTGCCCTGGGCCACTTCAAACAAAGAACCATCGCGGGTGAAA
TTGGTTCTTCTACCATGCCACACAAAGTCAACCCAATCGACTTTGAAAACTCAGAAGGTAACTTGGGTCTGGCGAATGCG
GTATTTACCCATTTAGCGCAAAAGCTGCCTATCTCTCGTTGGCAACGTGACCTAACGGATTCTACCGTGCTGCGTAACCT
CGGTGTGGGTGTCGGCTATGCCATCATCGCTTATACCTCAACTTTGAAAGGCATCAGCAAGCTGGAAGTGAACCGTGACG
CACTACTTGCAGAACTGGATCACAACTGGGAAGTTCTGGCAGAGCCAATCCAAACTGTGATGCGCCGCTACGGCATTGAA
AAGCCATATGAGAAGCTCAAAGAACTGACTCGTGGTAAGCGAGTAGATGGCGAAGCGATGCGTCAATTCATTGATGGTTT
AGCACTGCCAGCGGAAGAGAAAACTCGCTTGAAAGCCATGACACCAGCCAGCTATATTGGCTACGCGATTGAGCTGACGG
ACAAGCTGTAA

Upstream 100 bases:

>100_bases
AAAGATGATCGAACAAGCGCAGATTTTGTTAGCGCGAATCTAAGTGATGACTACCCACTCGAATTACTCACACCTTTAAC
TCAATATTCAGGAGAAGAAC

Downstream 100 bases:

>100_bases
TCTCGACAAGAGAGACCAAATGACTAAGGGGAGCAACGGCTCCCCTTTTACTTATCGACGACTTATCGACTTGGTTTATA
CCAGACCGTTTCACTCCTGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFSAEANQFLDAIAANFNEADAL
RIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIE
KPYEKLKELTRGKRVDGEAMRQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL

Sequences:

>Translated_456_residues
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFSAEANQFLDAIAANFNEADAL
RIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIE
KPYEKLKELTRGKRVDGEAMRQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL
>Mature_456_residues
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFSAEANQFLDAIAANFNEADAL
RIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFACTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
VFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTSTLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIE
KPYEKLKELTRGKRVDGEAMRQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=82.4561403508772, Blast_Score=790, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323391, Length=346, Percent_Identity=24.2774566473988, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647570, Length=271, Percent_Identity=26.5682656826568, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51335; Mature: 51335

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AEANQFLDAIAANFNEADALRIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAVFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDGEAM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL
HHHHHHCCCCCCHHHHHHHCCCHHHHCEEEEEECCC
>Mature Secondary Structure
MELSALTAVSPVDGRYGSKTIALRSIFSEFGLLKYRTIVEIRWLQKLAATAEIAEVPAFS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AEANQFLDAIAANFNEADALRIKEIERTTNHDVKAVEYFLKEKVAAMPELHAVNEFIHFA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTVILPEIRNVIDAIRKLAEEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIANVEILAKINGAVGNYNAHLSAYPTVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCC
DWNPYTTQIEPHDYIAELFEAVARFNTILIDFDRDVWGYIALGHFKQRTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAVFTHLAQKLPISRWQRDLTDSTVLRNLGVGVGYAIIAYTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TLKGISKLEVNRDALLAELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDGEAM
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RQFIDGLALPAEEKTRLKAMTPASYIGYAIELTDKL
HHHHHHCCCCCCHHHHHHHCCCHHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]