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The map label for this gene is bioD [H]

Identifier: 227081288

GI number: 227081288

Start: 1142361

End: 1143077

Strand: Direct

Name: bioD [H]

Synonym: VCM66_1071

Alternate gene names: 227081288

Gene position: 1142361-1143077 (Clockwise)

Preceding gene: 227081287

Following gene: 227081290

Centisome position: 39.49

GC content: 47.98

Gene sequence:

>717_bases
TTGTTTAGGGGTTATTCACCGATGAGAAATGCAATTTTTATCGCAGGGACGGATACCGACGTTGGGAAAACAGTCGCTTC
GAAAGCGATTCTTCAGGCGCTCGCGACACACAATATCGCTACGATTGGCTATAAGCCAGTCGCAGCGGGGAGTGATAAAA
CTGAGTTTGGCTATCGCAATTCCGATGCCTTGCATCTGATGAAAGCCGCGACCGTCGATATGCCGTATGAGGACGTCAAT
CCTTATGCCTTAGTACTTCCCACTTCACCACACATCGCCGCTAAGCATGAAAATGTTACGATTGATTATGCGCTGCTAAG
CAATAAGCTCTCGAAACATAAGCAAAATGCAGAGTTAGTCGTGGTAGAAGGTGCGGGAGGTTGGCGAGTTCCTACTTCCG
ATTCCGATTGTTTATCTACTTGGGTAAAACAAGAGCGTTTACCGGTCATCTTGGTGGTTGGAATTAAACTCGGTTGTCTG
AGCCATGCGATTTTGACCGCAGAAGCGATTCGTGCCGATGGGTTAGAGCTAGTCGGCTGGATTGCCAACCGGATTAATCC
GGGAACTGAACACTATGCGGAGATCATTGAGCATCTAGAAGGGCGGCTTGGTACACCGAAATTAGGGGAGATCCCTTATA
TGCCTAAGGCTAAACGTCAAGAGTTAGGCAAGTTCATCCAACTGGATCACTTGTTAGAACCCGACACCGTGGCATAA

Upstream 100 bases:

>100_bases
GTTCAACGGGGCTAATCAGTCGCCGAACGTTGGCAAAAGTGGAATTGGCCTACCAATCATTTCGTAACCAACAGGGTCTT
GTGCCTGCCACATATCAGGT

Downstream 100 bases:

>100_bases
CGACGCTAAATAACAAAAGGCTGCTCAAACATGAGCAGCCTTTTTAGTATCAGACTGTATGGAAGTTGAGTGTGCGAGAG
GCTATTTCTCTGCGCTCAAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MFRGYSPMRNAIFIAGTDTDVGKTVASKAILQALATHNIATIGYKPVAAGSDKTEFGYRNSDALHLMKAATVDMPYEDVN
PYALVLPTSPHIAAKHENVTIDYALLSNKLSKHKQNAELVVVEGAGGWRVPTSDSDCLSTWVKQERLPVILVVGIKLGCL
SHAILTAEAIRADGLELVGWIANRINPGTEHYAEIIEHLEGRLGTPKLGEIPYMPKAKRQELGKFIQLDHLLEPDTVA

Sequences:

>Translated_238_residues
MFRGYSPMRNAIFIAGTDTDVGKTVASKAILQALATHNIATIGYKPVAAGSDKTEFGYRNSDALHLMKAATVDMPYEDVN
PYALVLPTSPHIAAKHENVTIDYALLSNKLSKHKQNAELVVVEGAGGWRVPTSDSDCLSTWVKQERLPVILVVGIKLGCL
SHAILTAEAIRADGLELVGWIANRINPGTEHYAEIIEHLEGRLGTPKLGEIPYMPKAKRQELGKFIQLDHLLEPDTVA
>Mature_238_residues
MFRGYSPMRNAIFIAGTDTDVGKTVASKAILQALATHNIATIGYKPVAAGSDKTEFGYRNSDALHLMKAATVDMPYEDVN
PYALVLPTSPHIAAKHENVTIDYALLSNKLSKHKQNAELVVVEGAGGWRVPTSDSDCLSTWVKQERLPVILVVGIKLGCL
SHAILTAEAIRADGLELVGWIANRINPGTEHYAEIIEHLEGRLGTPKLGEIPYMPKAKRQELGKFIQLDHLLEPDTVA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=224, Percent_Identity=52.2321428571429, Blast_Score=233, Evalue=6e-63,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=48, Blast_Score=210, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25931; Mature: 25931

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGYSPMRNAIFIAGTDTDVGKTVASKAILQALATHNIATIGYKPVAAGSDKTEFGYRN
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCEEECCCCCCCCCCCCC
SDALHLMKAATVDMPYEDVNPYALVLPTSPHIAAKHENVTIDYALLSNKLSKHKQNAELV
CCHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCEEEEHHHHHHHHHHHCCCCCEE
VVEGAGGWRVPTSDSDCLSTWVKQERLPVILVVGIKLGCLSHAILTAEAIRADGLELVGW
EEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHH
IANRINPGTEHYAEIIEHLEGRLGTPKLGEIPYMPKAKRQELGKFIQLDHLLEPDTVA
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFRGYSPMRNAIFIAGTDTDVGKTVASKAILQALATHNIATIGYKPVAAGSDKTEFGYRN
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCEEECCCCCCCCCCCCC
SDALHLMKAATVDMPYEDVNPYALVLPTSPHIAAKHENVTIDYALLSNKLSKHKQNAELV
CCHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCEEEEHHHHHHHHHHHCCCCCEE
VVEGAGGWRVPTSDSDCLSTWVKQERLPVILVVGIKLGCLSHAILTAEAIRADGLELVGW
EEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHH
IANRINPGTEHYAEIIEHLEGRLGTPKLGEIPYMPKAKRQELGKFIQLDHLLEPDTVA
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]