Click here to switch to the map view.

The map label for this gene is gap [H]

Identifier: 227081243

GI number: 227081243

Start: 1093732

End: 1094733

Strand: Direct

Name: gap [H]

Synonym: VCM66_1025

Alternate gene names: 227081243

Gene position: 1093732-1094733 (Clockwise)

Preceding gene: 227081242

Following gene: 227081244

Centisome position: 37.81

GC content: 48.0

Gene sequence:

>1002_bases
ATGGCAATTAAAGTCGGAATTAATGGATTTGGTCGTATTGGGCGTTTGGCTTTAAGAGCTTCGTTTGATTGGCCTGAGAT
TGAGTTTGTACAGATCAACGATGTGGCGGGTGATGCTGCAACATTAGCGCACCTTTTGGAGTTCGATTCGGTACAAGGTC
GTTGGCATCATGCTGTGGGCAGTGAAGCGGATGCTATCCTGATCAACGGCAAACGCATTCGCACAACACAAGAAAAATCG
ATTGATGCAGTGGATTGGTCGGGCTGTGATGTGGTGATTGAGGCTACGGGTAAGCACCGTAAAGGCGAATTCTTGAACCA
GTATTTGGCACAAGGAGTAAAACGCGTCGTCGTTTCAGCGCCTGTCAAAGAAGAGGGGATCGCCAATATCGTGGTCGGGG
TGAACGATCACATCTTCAATCCGGAGCAACATCGCATTGTCACTGCCGCTTCTTGTACCACTAACTGTATTGCTCCAGTG
GTGAAAGTAATCCATGAAAAACTGGGTATCGCACAAGCCTCTTTTACTACCATCCATAACCTAACCAATACGCAAACCAT
TTTGGATGCACCGCATAAAGATTTACGCCGTGCACGTGCTTGCGGTATGAGCCTCATCCCCACAACCACCGGCTCTGCTA
AAGCGATTATCGAAATTTTCCCGGATCTGAAAGGCAAGATTGATGGACACGCCGTACGTGTACCGTTGGCGAATGCCTCG
TTAACCGACATCATTTTTGATGTGCAGCGTGATACGACGGTGGAAGAGATCAATCAGCTGTTAAAACAAGCCTCAGAAAA
CGAGCTTAAAGGCATTCTGGGTTTTGAAGAGCGTCCACTGGTGTCGATTGATTACCAAGGCGATCAACGTTCAACAATTG
TGGATGCGCTCTCCACCATGGTGGTTGGTAAGCGAATGGTGAAAATCTACACTTGGTATGACAACGAAATGGGTTACGCC
ACGCGCACCGCGGAATTGGTTCGTAAAGTCGGCTTGGCATAA

Upstream 100 bases:

>100_bases
GTAAAGAACCAAGTTGAGTTATCCCTCAATGACTTAGCGTTGTAGCCACGATGGCTGCTTAGTTGGGAAGGGATAAAAAC
GAGTTTAAAGGAAAAGGAAG

Downstream 100 bases:

>100_bases
GGAGTAGTAAAAAATGACGCATCCAACTTGGGAATTACCGGTAGCTGACAATCAGGCGAGCTTAGTGCTCACACCTTGCC
CGGGAACCAAAGGTACACCA

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAILINGKRIRTTQEKS
IDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPV
VKVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYTWYDNEMGYA
TRTAELVRKVGLA

Sequences:

>Translated_333_residues
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAILINGKRIRTTQEKS
IDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPV
VKVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYTWYDNEMGYA
TRTAELVRKVGLA
>Mature_332_residues
AIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVGSEADAILINGKRIRTTQEKSI
DAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSAPVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVV
KVIHEKLGIAQASFTTIHNLTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANASL
TDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTMVVGKRMVKIYTWYDNEMGYAT
RTAELVRKVGLA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=328, Percent_Identity=39.3292682926829, Blast_Score=257, Evalue=9e-69,
Organism=Homo sapiens, GI7657116, Length=329, Percent_Identity=37.0820668693009, Blast_Score=239, Evalue=4e-63,
Organism=Escherichia coli, GI1788079, Length=330, Percent_Identity=40.3030303030303, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1789295, Length=327, Percent_Identity=40.3669724770642, Blast_Score=244, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI32566163, Length=334, Percent_Identity=37.125748502994, Blast_Score=240, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI17568413, Length=334, Percent_Identity=37.125748502994, Blast_Score=240, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17534677, Length=331, Percent_Identity=36.2537764350453, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17534679, Length=331, Percent_Identity=35.6495468277946, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321631, Length=336, Percent_Identity=39.5833333333333, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6322468, Length=334, Percent_Identity=38.0239520958084, Blast_Score=255, Evalue=8e-69,
Organism=Saccharomyces cerevisiae, GI6322409, Length=336, Percent_Identity=38.0952380952381, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19922412, Length=326, Percent_Identity=37.1165644171779, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI85725000, Length=328, Percent_Identity=37.5, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI22023983, Length=328, Percent_Identity=37.5, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI17933600, Length=328, Percent_Identity=36.5853658536585, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI18110149, Length=328, Percent_Identity=36.5853658536585, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36487; Mature: 36356

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVG
CEEEEECCCCCCHHEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHCCC
SEADAILINGKRIRTTQEKSIDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSA
CCCCEEEECCCEEECCHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVVKVIHEKLGIAQASFTTIHN
CCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHEEECCCCCCCCCCEEEEEEECCCC
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTM
HHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
VVGKRMVKIYTWYDNEMGYATRTAELVRKVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKVGINGFGRIGRLALRASFDWPEIEFVQINDVAGDAATLAHLLEFDSVQGRWHHAVG
EEEEECCCCCCHHEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHCCC
SEADAILINGKRIRTTQEKSIDAVDWSGCDVVIEATGKHRKGEFLNQYLAQGVKRVVVSA
CCCCEEEECCCEEECCHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
PVKEEGIANIVVGVNDHIFNPEQHRIVTAASCTTNCIAPVVKVIHEKLGIAQASFTTIHN
CCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
LTNTQTILDAPHKDLRRARACGMSLIPTTTGSAKAIIEIFPDLKGKIDGHAVRVPLANAS
CCCCHHHHCCCHHHHHHHHHCCCEEEECCCCCHHHHEEECCCCCCCCCCEEEEEEECCCC
LTDIIFDVQRDTTVEEINQLLKQASENELKGILGFEERPLVSIDYQGDQRSTIVDALSTM
HHHHEEECCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
VVGKRMVKIYTWYDNEMGYATRTAELVRKVGLA
HHHHEEEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]