BacMap

Definition	Vibrio cholerae M66-2 chromosome I, complete genome.
Accession	NC_012578
Length	2,892,523

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The map label for this gene is yciK [H]

Identifier: 227081232

GI number: 227081232

Start: 1084248

End: 1084991

Strand: Reverse

Name: yciK [H]

Synonym: VCM66_1014

Alternate gene names: 227081232

Gene position: 1084991-1084248 (Counterclockwise)

Preceding gene: 227081234

Following gene: 227081230

Centisome position: 37.51

GC content: 48.39

Gene sequence:

>744_bases
GTGAATTATTCAGTCTCAACAGACGCTTTGAAAGAGAAAGTGATTTTAGTCACCGGAGCGGGTGATGGTATTGGTAAGCA
AGCGGCGATCAGTTTCGCGGCGCACGGCGCGACCGTGATTTTACTTGGCCGTACCGTGAAAAAGCTCGAACAAACCTATG
ATGCTATTGAAGCAGCGGGTTATCCGACACCGGCGATTGTTCCTTTAGATATGAAAGGCGCAACCAAACAAAATTATCTG
GATATGGTCGATACAATAGAAGATCAATTTGGCCGTTTGGATGGCGTATTACACAATGCCAGCTTGCTTGGCGTACTCAG
CCCCTTCGATCAAATTGGCGAAGACAGCTTCGATGAAGTGATGCAAGTGAACGTGAAAGCACAGTTCCTTATGACTCAAG
CGCTGCTACCGCTGCTGCACAAATCAGCCGATGCTCGAATTGTGTTTACCACGTCTACCGTAGGTCATATTGGCCGTGCT
TTCTGGGCAACTTACGCGATTTCTAAGTTTGCGACTGAAGGTATGATGCAAATTCTGGCCGATGAATTGAGTGAAAGCAC
CATTCGAGTCAATGCGATTAACCCGGGCGGCACTCGCACCGCGATGCGCGCGAAAGCCTACCCAGCAGAGGATGCAGACA
AGTTAAAAACACCGCTCGATATTATGCCACTCTATCTGTACTTGATGGCTCCAGAAGGCCGTGAAGTGCACGGTCAATGT
ATTGATGCCCAGCCAAAGAAATAA

Upstream 100 bases:

>100_bases
TAAAGCAAATCCTCTTCCAACTCGACTAACCTTGGATTTTTGACACGCCTCAGCCAGAGTTTGCCAGCAGGAAGAGCTAA
AAAATAATAAGGAATAAGAC

Downstream 100 bases:

>100_bases
CGCGGTTTAGTTTTCGCTTTCACACAAAGACAAACGCCTTGCAAGCAGCAAGGCGTTTTTGTTCTCTAACATCACCATCG
ATGAGTAAAGCGCTTAATCT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MNYSVSTDALKEKVILVTGAGDGIGKQAAISFAAHGATVILLGRTVKKLEQTYDAIEAAGYPTPAIVPLDMKGATKQNYL
DMVDTIEDQFGRLDGVLHNASLLGVLSPFDQIGEDSFDEVMQVNVKAQFLMTQALLPLLHKSADARIVFTTSTVGHIGRA
FWATYAISKFATEGMMQILADELSESTIRVNAINPGGTRTAMRAKAYPAEDADKLKTPLDIMPLYLYLMAPEGREVHGQC
IDAQPKK

Sequences:

>Translated_247_residues
MNYSVSTDALKEKVILVTGAGDGIGKQAAISFAAHGATVILLGRTVKKLEQTYDAIEAAGYPTPAIVPLDMKGATKQNYL
DMVDTIEDQFGRLDGVLHNASLLGVLSPFDQIGEDSFDEVMQVNVKAQFLMTQALLPLLHKSADARIVFTTSTVGHIGRA
FWATYAISKFATEGMMQILADELSESTIRVNAINPGGTRTAMRAKAYPAEDADKLKTPLDIMPLYLYLMAPEGREVHGQC
IDAQPKK
>Mature_247_residues
MNYSVSTDALKEKVILVTGAGDGIGKQAAISFAAHGATVILLGRTVKKLEQTYDAIEAAGYPTPAIVPLDMKGATKQNYL
DMVDTIEDQFGRLDGVLHNASLLGVLSPFDQIGEDSFDEVMQVNVKAQFLMTQALLPLLHKSADARIVFTTSTVGHIGRA
FWATYAISKFATEGMMQILADELSESTIRVNAINPGGTRTAMRAKAYPAEDADKLKTPLDIMPLYLYLMAPEGREVHGQC
IDAQPKK

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI157739938, Length=199, Percent_Identity=30.1507537688442, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4504505, Length=235, Percent_Identity=30.6382978723404, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI32483357, Length=218, Percent_Identity=28.8990825688073, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI10190704, Length=247, Percent_Identity=28.3400809716599, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI223718074, Length=210, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI15277342, Length=232, Percent_Identity=28.448275862069, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI20149619, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI7705925, Length=216, Percent_Identity=29.1666666666667, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI126723191, Length=194, Percent_Identity=27.8350515463918, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI210032110, Length=212, Percent_Identity=24.5283018867925, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI142976729, Length=174, Percent_Identity=28.1609195402299, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI19923817, Length=254, Percent_Identity=22.8346456692913, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4503817, Length=189, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI150247226, Length=242, Percent_Identity=28.099173553719, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=23.4567901234568, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787526, Length=247, Percent_Identity=53.4412955465587, Blast_Score=248, Evalue=2e-67,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=30.7086614173228, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=28.4584980237154, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=24.1935483870968, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790717, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787905, Length=239, Percent_Identity=29.7071129707113, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1786701, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787335, Length=237, Percent_Identity=24.8945147679325, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=25, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786812, Length=204, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=208, Percent_Identity=31.25, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=201, Percent_Identity=30.3482587064677, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=220, Percent_Identity=33.1818181818182, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=195, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=198, Percent_Identity=31.3131313131313, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=235, Percent_Identity=28.0851063829787, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=193, Percent_Identity=30.0518134715026, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=208, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=189, Percent_Identity=30.6878306878307, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=242, Percent_Identity=26.4462809917355, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536651, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=223, Percent_Identity=29.5964125560538, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=202, Percent_Identity=27.2277227722772, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=241, Percent_Identity=31.5352697095436, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=211, Percent_Identity=26.0663507109005, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17536025, Length=190, Percent_Identity=31.0526315789474, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=199, Percent_Identity=27.1356783919598, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562990, Length=208, Percent_Identity=30.2884615384615, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=193, Percent_Identity=25.9067357512953, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=184, Percent_Identity=29.3478260869565, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534660, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=200, Percent_Identity=31, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567345, Length=206, Percent_Identity=27.1844660194175, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=208, Percent_Identity=28.8461538461538, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=193, Percent_Identity=26.9430051813472, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=222, Percent_Identity=25.6756756756757, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557780, Length=195, Percent_Identity=25.1282051282051, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=229, Percent_Identity=30.5676855895196, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323882, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571526, Length=192, Percent_Identity=35.9375, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=34.5744680851064, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=31.9672131147541, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644339, Length=211, Percent_Identity=32.7014218009479, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357041, Length=190, Percent_Identity=32.1052631578947, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=26.1044176706827, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358495, Length=203, Percent_Identity=29.064039408867, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364892, Length=194, Percent_Identity=26.2886597938144, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=27.0161290322581, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640492, Length=184, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI18859837, Length=240, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45553409, Length=197, Percent_Identity=29.4416243654822, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647948, Length=197, Percent_Identity=29.4416243654822, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357673, Length=249, Percent_Identity=27.710843373494, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24647946, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26735; Mature: 26735

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSVSTDALKEKVILVTGAGDGIGKQAAISFAAHGATVILLGRTVKKLEQTYDAIEAAG
CCCCCCHHHHHCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCC
YPTPAIVPLDMKGATKQNYLDMVDTIEDQFGRLDGVLHNASLLGVLSPFDQIGEDSFDEV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
MQVNVKAQFLMTQALLPLLHKSADARIVFTTSTVGHIGRAFWATYAISKFATEGMMQILA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELSESTIRVNAINPGGTRTAMRAKAYPAEDADKLKTPLDIMPLYLYLMAPEGREVHGQC
HHHCCCEEEEEEECCCCCCHHHEECCCCCCCHHHHCCCHHHHHHHHHEECCCCCCCCCEE
IDAQPKK
CCCCCCC
>Mature Secondary Structure
MNYSVSTDALKEKVILVTGAGDGIGKQAAISFAAHGATVILLGRTVKKLEQTYDAIEAAG
CCCCCCHHHHHCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCC
YPTPAIVPLDMKGATKQNYLDMVDTIEDQFGRLDGVLHNASLLGVLSPFDQIGEDSFDEV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHH
MQVNVKAQFLMTQALLPLLHKSADARIVFTTSTVGHIGRAFWATYAISKFATEGMMQILA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DELSESTIRVNAINPGGTRTAMRAKAYPAEDADKLKTPLDIMPLYLYLMAPEGREVHGQC
HHHCCCEEEEEEECCCCCCHHHEECCCCCCCHHHHCCCHHHHHHHHHEECCCCCCCCCEE
IDAQPKK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases; Acting on the CH-OH group of donors; With NAD or NADP as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1885549 [H]