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The map label for this gene is fadI [H]

Identifier: 227081219

GI number: 227081219

Start: 1072184

End: 1073638

Strand: Direct

Name: fadI [H]

Synonym: VCM66_1001

Alternate gene names: 227081219

Gene position: 1072184-1073638 (Clockwise)

Preceding gene: 227081214

Following gene: 227081220

Centisome position: 37.07

GC content: 49.21

Gene sequence:

>1455_bases
TTGCAAAAAATCCTACCAACACCGGTTTGTCAGTGTTTTTCATCTCGAAAGGATTGTTTAAATCACGATTATTTAAACGG
CTGTTTGATTTGTTTAACATTCTCGTTACAATGCGCCAGGTCAGACCTCTTATTCGAAGGAGAAATCATGGGCAAACAGG
AAGTCAGGACTCGTTCTGGAGAACGAGTGGCGATTGTGGCCGGGCTACGTACGCCGTTTGCGCGTCAAAGCACCGAGTTT
GGTCAAGTACCCGCAGTCGATCTCGGCAAAATGGTAGTACAAGAGATGATGGCGCGCACCGCTATCGATCCTAAATTAAT
TGAGCAAGTGGTGTTTGGCCAAGTGGTACAGATGCCAGAAGCGCCGAATATTGCTCGAGAAATTGTACTCGGTACCGGAA
TGTCGATTAACACGGATGCCTACAGCGTTACTCGCGCTTGCGCCACCAGTTTTCAAGCCGCCGTCAATGTGGCCGAAAGT
ATCATGGCTGGCAGCATAGATATTGGTATTGCAGGTGGTGCGGATTCTTCTTCTGTTCTGCCGATTGGTGTTTCTAAAAA
ACTGGCGGCTTCTTTATTAGCCTTGAGTAAAACTAAGACGGTCGGGCAGAAACTTAAACTGCTTAGTAACCTCTCTTTTA
AAGACTTGATGCCCGTACCTCCTGCGGTCGCGGAATATTCAACGGGTCTGTCGATGGGGCAAACTGCTGAGCAAATGGCC
AAAAGCTATGCGATCTCTCGCGCTGAGCAAGATGCGTTAGCACATCGTTCACATACTTTAGCGGCTCAAGCATGGGCAGA
GGGCAAAATTCGCGATGAAGTGATGACGGCTTTTCCTGAGCCATACAAAAAATGGCTGGATATGGATAACAACATTCGCA
TGGACTCCAAGCTAGAGAGCTATGCAAAGTTGCGTCCTGCCTTTGACCGTCAATACGGTTCGGTGACCGCAGCAAACTCA
ACCCCACTGACGGATGGCGCCGCCGCGATTATGTTGATGCGTGAAGGTAAAGCGAAAGAGCTTGGTCTTGAGATCATGGG
GTATATCCGCTCTTATGCGTTTGCCGCGATTGGCGTAGAAAAAGATATGCTGATGGGGCCTTCTTACGCCACTCCGATAG
CCTTGGATCGCGCAGGCATCACCTTAAATGATCTCACCTTGATTGATATGCACGAAGCCTTTGCCGCGCAAACCTTAGCG
AACTTGAAGATGTTCGCCAGTGACAAGTTTGCTCAAGAGCAGCTAGGTCGCGCACAAGCGATTGGTGAAGTGGACATGAG
TAAATTTAACGTGCTGGGTGGCTCACTGGCTTATGGTCACCCATTCGCGGCGACAGGTGCGCGTATGATCACTCAAACTT
TGCGTGAATTAAAACGCCGTGGCGGTGGTTTAGCCTTAAATACGGCTTGTGCTGCGGGTGGCTTAGGCGCTGCTATGATT
CTGGAGGTGGAATAA

Upstream 100 bases:

>100_bases
AGGCTGACTTTACTTTCTCCTAATCAGGAAACTGATTTTTCAATTGGCGTAAGTTAATTGCTTTACCCATTCCGACCACA
CAGTTCTGTGTGGTTTTTTT

Downstream 100 bases:

>100_bases
TGGATAACAACAATGCATTTCAGCTCTCGTTTGATGAGCAGCATTACGCTTGGCTGGCGATTGATGTCCCGGGCGAAAAA
ATGAATACCCTGCAAGCGGC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQKILPTPVCQCFSSRKDCLNHDYLNGCLICLTFSLQCARSDLLFEGEIMGKQEVRTRSGERVAIVAGLRTPFARQSTEF
GQVPAVDLGKMVVQEMMARTAIDPKLIEQVVFGQVVQMPEAPNIAREIVLGTGMSINTDAYSVTRACATSFQAAVNVAES
IMAGSIDIGIAGGADSSSVLPIGVSKKLAASLLALSKTKTVGQKLKLLSNLSFKDLMPVPPAVAEYSTGLSMGQTAEQMA
KSYAISRAEQDALAHRSHTLAAQAWAEGKIRDEVMTAFPEPYKKWLDMDNNIRMDSKLESYAKLRPAFDRQYGSVTAANS
TPLTDGAAAIMLMREGKAKELGLEIMGYIRSYAFAAIGVEKDMLMGPSYATPIALDRAGITLNDLTLIDMHEAFAAQTLA
NLKMFASDKFAQEQLGRAQAIGEVDMSKFNVLGGSLAYGHPFAATGARMITQTLRELKRRGGGLALNTACAAGGLGAAMI
LEVE

Sequences:

>Translated_484_residues
MQKILPTPVCQCFSSRKDCLNHDYLNGCLICLTFSLQCARSDLLFEGEIMGKQEVRTRSGERVAIVAGLRTPFARQSTEF
GQVPAVDLGKMVVQEMMARTAIDPKLIEQVVFGQVVQMPEAPNIAREIVLGTGMSINTDAYSVTRACATSFQAAVNVAES
IMAGSIDIGIAGGADSSSVLPIGVSKKLAASLLALSKTKTVGQKLKLLSNLSFKDLMPVPPAVAEYSTGLSMGQTAEQMA
KSYAISRAEQDALAHRSHTLAAQAWAEGKIRDEVMTAFPEPYKKWLDMDNNIRMDSKLESYAKLRPAFDRQYGSVTAANS
TPLTDGAAAIMLMREGKAKELGLEIMGYIRSYAFAAIGVEKDMLMGPSYATPIALDRAGITLNDLTLIDMHEAFAAQTLA
NLKMFASDKFAQEQLGRAQAIGEVDMSKFNVLGGSLAYGHPFAATGARMITQTLRELKRRGGGLALNTACAAGGLGAAMI
LEVE
>Mature_484_residues
MQKILPTPVCQCFSSRKDCLNHDYLNGCLICLTFSLQCARSDLLFEGEIMGKQEVRTRSGERVAIVAGLRTPFARQSTEF
GQVPAVDLGKMVVQEMMARTAIDPKLIEQVVFGQVVQMPEAPNIAREIVLGTGMSINTDAYSVTRACATSFQAAVNVAES
IMAGSIDIGIAGGADSSSVLPIGVSKKLAASLLALSKTKTVGQKLKLLSNLSFKDLMPVPPAVAEYSTGLSMGQTAEQMA
KSYAISRAEQDALAHRSHTLAAQAWAEGKIRDEVMTAFPEPYKKWLDMDNNIRMDSKLESYAKLRPAFDRQYGSVTAANS
TPLTDGAAAIMLMREGKAKELGLEIMGYIRSYAFAAIGVEKDMLMGPSYATPIALDRAGITLNDLTLIDMHEAFAAQTLA
NLKMFASDKFAQEQLGRAQAIGEVDMSKFNVLGGSLAYGHPFAATGARMITQTLRELKRRGGGLALNTACAAGGLGAAMI
LEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=42.3809523809524, Blast_Score=338, Evalue=1e-92,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=32.093023255814, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI4557237, Length=426, Percent_Identity=30.2816901408451, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI167614485, Length=427, Percent_Identity=29.9765807962529, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI148539872, Length=426, Percent_Identity=29.1079812206573, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1788683, Length=428, Percent_Identity=73.1308411214953, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=423, Percent_Identity=33.3333333333333, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI48994986, Length=429, Percent_Identity=31.4685314685315, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788554, Length=421, Percent_Identity=33.4916864608076, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=32.7058823529412, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=38.785046728972, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI133906874, Length=428, Percent_Identity=31.3084112149533, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=425, Percent_Identity=26.8235294117647, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17535921, Length=428, Percent_Identity=27.3364485981308, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17535917, Length=427, Percent_Identity=22.248243559719, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322031, Length=447, Percent_Identity=29.7539149888143, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=435, Percent_Identity=28.5057471264368, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137578, Length=432, Percent_Identity=41.8981481481481, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24655093, Length=427, Percent_Identity=29.7423887587822, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=27.2941176470588, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17648125, Length=425, Percent_Identity=28, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 51905; Mature: 51905

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKILPTPVCQCFSSRKDCLNHDYLNGCLICLTFSLQCARSDLLFEGEIMGKQEVRTRSG
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCC
ERVAIVAGLRTPFARQSTEFGQVPAVDLGKMVVQEMMARTAIDPKLIEQVVFGQVVQMPE
CEEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
APNIAREIVLGTGMSINTDAYSVTRACATSFQAAVNVAESIMAGSIDIGIAGGADSSSVL
CCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEE
PIGVSKKLAASLLALSKTKTVGQKLKLLSNLSFKDLMPVPPAVAEYSTGLSMGQTAEQMA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCCCHHHHHHH
KSYAISRAEQDALAHRSHTLAAQAWAEGKIRDEVMTAFPEPYKKWLDMDNNIRMDSKLES
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHH
YAKLRPAFDRQYGSVTAANSTPLTDGAAAIMLMREGKAKELGLEIMGYIRSYAFAAIGVE
HHHHCHHHHHCCCCEEECCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
KDMLMGPSYATPIALDRAGITLNDLTLIDMHEAFAAQTLANLKMFASDKFAQEQLGRAQA
HHHHCCCCCCCCEEECCCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGEVDMSKFNVLGGSLAYGHPFAATGARMITQTLRELKRRGGGLALNTACAAGGLGAAMI
HCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCEEEE
LEVE
EEEC
>Mature Secondary Structure
MQKILPTPVCQCFSSRKDCLNHDYLNGCLICLTFSLQCARSDLLFEGEIMGKQEVRTRSG
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCC
ERVAIVAGLRTPFARQSTEFGQVPAVDLGKMVVQEMMARTAIDPKLIEQVVFGQVVQMPE
CEEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
APNIAREIVLGTGMSINTDAYSVTRACATSFQAAVNVAESIMAGSIDIGIAGGADSSSVL
CCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEE
PIGVSKKLAASLLALSKTKTVGQKLKLLSNLSFKDLMPVPPAVAEYSTGLSMGQTAEQMA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHCCCCCCHHHHHHH
KSYAISRAEQDALAHRSHTLAAQAWAEGKIRDEVMTAFPEPYKKWLDMDNNIRMDSKLES
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHH
YAKLRPAFDRQYGSVTAANSTPLTDGAAAIMLMREGKAKELGLEIMGYIRSYAFAAIGVE
HHHHCHHHHHCCCCEEECCCCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
KDMLMGPSYATPIALDRAGITLNDLTLIDMHEAFAAQTLANLKMFASDKFAQEQLGRAQA
HHHHCCCCCCCCEEECCCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGEVDMSKFNVLGGSLAYGHPFAATGARMITQTLRELKRRGGGLALNTACAAGGLGAAMI
HCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCEEEE
LEVE
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA