Click here to switch to the map view.

The map label for this gene is fadL-2 [H]

Identifier: 227081216

GI number: 227081216

Start: 1068967

End: 1070253

Strand: Reverse

Name: fadL-2 [H]

Synonym: VCM66_0998

Alternate gene names: 227081216

Gene position: 1070253-1068967 (Counterclockwise)

Preceding gene: 227081217

Following gene: 227081215

Centisome position: 37.0

GC content: 45.69

Gene sequence:

>1287_bases
ATGAACAACATGCGTCTGTTTAAAAAATCTCTGCTTGCCGTGACGGTAACGCTAGCCACACAACAAGCGTTTGCAGCAGG
TTTCCAGCTCAACGCACAATCTGCTACCGGCCTAGGTCGCGCTTTTGCGGGTGATGCCGTTATCGCCGATAACGCAGCCG
TAATGTCTCGCAACCCAGCGGCGATGGCACTGTTTGATGAGATGGCGCTCTCTCTTGGTTTTGAAACCATTACCACCATG
ATTGACGTTAAAAATGTCTCTTATCGAGGTGTTCTCGGTAACTCAGTTTCTGTTCAAGACACGGATGACGTAGGTGGTAC
TTCCGTTGCACCTAATATTCATTTGATCGTTCCAGTCAATGAACAGTTTGCTTGGGGGGTTAATGCTTATTCAAACTTTG
GTACAAAAACTGAGTTTTCTGATTCTTATCCAGCCTCTGAATACGGTGGCTTAACAGACGTTAAAAGCTTTAACTTAGGC
CTAGCAGGTTCTTATCGCGTCAACAAAGAATGGAGTTTTGGTGCTGGTTTAGACCTGATTTATGGGCAAGGCACCATGAA
ACGTATTGCCGGAACCAGTTTCCCAGCAAGTACAATTGTGGTGAACCCGACGACCGTTATCCCAGCTCGAGGCACATCAC
TACTCAACGTCGATAAAGCGGATGGTTGGGCAGTAGGTTTTAACTTGGGTACAGTTTACGAAATGGATGAGAATAACCGC
TTTGGTTTGTCTTACCGCTATAGCCCAGAATTTAAAGCTAAAGACGATTACGGACAAGAGATCACGCTACCACTACCGGA
TATCGCTGAGTTCTCTGGCTTCCACAAAATTGAGAATACTCAGTTTGCCGTACACTATAGCGTACAGTGGATTGGCTGGA
GTGCTTTCGACAAAATTGAGTTCCGCAACCTTGTAACGTCTCGCTCTTCTGTTGGCGCTCTAACCAAAGGCAGCTACGAT
AAGATGTATGAGTGGCAAGATGGCTGGCACTACTCTATCGGTGGCACATACTACTTAAATAGTGATTGGACACTGCGTGC
AGGCTATATGTACGATACTAGCGCACAAGATTCACGTACTTCTATCTCTGTTCCTGATTCAGATCGCCAGTGGTTATCAG
CAGGCTTTACCTATAACATCGATGACAAATCAAACGTAGACTTTGGATTTACTTACTTAATGGGTAAAGATGTCGCAGTT
TCCGAAAATGATGCGGGTTCTAAGCTAACCGCAACAACCCACGCAGACGCAATCCTGTTGGGCTTACAATACAGCCGTAC
GTTTTAA

Upstream 100 bases:

>100_bases
ACTAGCGTCTAGGATCAGCCACCACTGAGCAATTGCTCAAATAAAGCAAAAGCTCATACAACAACGCCCATAAGAGGCGA
AACCAAAAAGGAAAAGTCTA

Downstream 100 bases:

>100_bases
TTAAAACGTCACAACATATCATTACTAAGGGGCTGATTTATCAGCCCCTTTCTTTTGCTGACTGCCTAGTTTTGCTTAAT
GTCAGCGTACAAGCGTGCGA

Product: long-chain fatty acid transport protein

Products: NA

Alternate protein names: Outer membrane fadL protein; Outer membrane flp protein [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKNVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF

Sequences:

>Translated_428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKNVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF
>Mature_428_residues
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPAAMALFDEMALSLGFETITTM
IDVKNVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVNEQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLG
LAGSYRVNKEWSFGAGLDLIYGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIEFRNLVTSRSSVGALTKGSYD
KMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRTSISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAV
SENDAGSKLTATTHADAILLGLQYSRTF

Specific function: Involved in translocation of long-chain fatty acids across the outer membrane. FadL may form a specific channel [H]

COG id: COG2067

COG function: function code I; Long-chain fatty acid transport protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ompP1/fadL family [H]

Homologues:

Organism=Escherichia coli, GI145693163, Length=440, Percent_Identity=35.4545454545455, Blast_Score=262, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR005017 [H]

Pfam domain/function: PF03349 Toluene_X [H]

EC number: NA

Molecular weight: Translated: 46797; Mature: 46797

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPA
CCCHHHHHHCEEEEEEEEEHHHHHHCCEEEECCCCCCCCHHHCCCEEEECCCEEECCCCC
AMALFDEMALSLGFETITTMIDVKNVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVN
HHHHHHHHHHHHCHHHHEEEEEECCCEEEEEECCCEEECCCCCCCCCEECCCEEEEEECC
EQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLGLAGSYRVNKEWSFGAGLDLI
CCEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCEEECCCCCCCCCEEEE
YGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
ECCCHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCE
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIE
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHCCCEEECCCEEEEEEEEEEEEECHHCHHH
FRNLVTSRSSVGALTKGSYDKMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRT
HHHHHHCCCCCCCCCCCCCHHCEECCCCEEEECCEEEEECCCEEEEEEEEECCCCCCCCC
SISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAVSENDAGSKLTATTHADAILL
EEECCCCCHHHHHCCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCEEEE
GLQYSRTF
EEEECCCC
>Mature Secondary Structure
MNNMRLFKKSLLAVTVTLATQQAFAAGFQLNAQSATGLGRAFAGDAVIADNAAVMSRNPA
CCCHHHHHHCEEEEEEEEEHHHHHHCCEEEECCCCCCCCHHHCCCEEEECCCEEECCCCC
AMALFDEMALSLGFETITTMIDVKNVSYRGVLGNSVSVQDTDDVGGTSVAPNIHLIVPVN
HHHHHHHHHHHHCHHHHEEEEEECCCEEEEEECCCEEECCCCCCCCCEECCCEEEEEECC
EQFAWGVNAYSNFGTKTEFSDSYPASEYGGLTDVKSFNLGLAGSYRVNKEWSFGAGLDLI
CCEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCCEEECCCCCCCCCEEEE
YGQGTMKRIAGTSFPASTIVVNPTTVIPARGTSLLNVDKADGWAVGFNLGTVYEMDENNR
ECCCHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEEECCCCEEEEEECCEEEEECCCCE
FGLSYRYSPEFKAKDDYGQEITLPLPDIAEFSGFHKIENTQFAVHYSVQWIGWSAFDKIE
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHCCCEEECCCEEEEEEEEEEEEECHHCHHH
FRNLVTSRSSVGALTKGSYDKMYEWQDGWHYSIGGTYYLNSDWTLRAGYMYDTSAQDSRT
HHHHHHCCCCCCCCCCCCCHHCEECCCCEEEECCEEEEECCCEEEEEEEEECCCCCCCCC
SISVPDSDRQWLSAGFTYNIDDKSNVDFGFTYLMGKDVAVSENDAGSKLTATTHADAILL
EEECCCCCHHHHHCCCEEECCCCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCEEEE
GLQYSRTF
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]