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The map label for this gene is gloA [H]

Identifier: 227081184

GI number: 227081184

Start: 1037336

End: 1037890

Strand: Reverse

Name: gloA [H]

Synonym: VCM66_0966

Alternate gene names: 227081184

Gene position: 1037890-1037336 (Counterclockwise)

Preceding gene: 227081185

Following gene: 227081183

Centisome position: 35.88

GC content: 46.85

Gene sequence:

>555_bases
ATGGCCGTTACACCTGTGTCGCGCGCAAACCACGTTGTGGCAGTTGCATTATCGAAGATCTTTGTGAATTCAAAGATAAA
GTGTACCCAGAAAGCTAATTTCCCAAAAAACCGTTATCCGGAAAGTAAGGAGCTCTTAATGTCCAACCATCGTATTTTGC
ACACCATGCTACGCGTCGGCGATTTGGATAAATCGATCGAATTCTATACTCAAGTAATGGGTATGAGCCTGCTGCGCAAA
AATGAGAATACTGAGTACAAATACACCCTCGCCTTCTTAGGTTATGGTGATGAATCCCAAGGTGCGGTCATTGAACTGAC
CTACAACTGGGGCGTGGCGGACTATGAGAAAGGCAACGCATACGGCCATATCGCGATTGGGGTTGATGATATTTATGCGA
CTTGCGATACCATCAAAGCAGCGGGAGGCATTGTAACCCGTGAACCAGGCCCAGTGAAAGGCGGGACAACTCACATCGCT
TTCGTGAAAGACCCTGATGGTTACATGATTGAGCTTATCCAAAACAAGCAAGCCCATGCAGGTCTTGAAGGCTAA

Upstream 100 bases:

>100_bases
AAATTTGCGGTCGGCAAAAACGTCGACGAAGTAGAGCACAAGCTACTCAAAGTCGTACCTAATGAGTTTAAACTCGATGT
GCACCACTGGCTGATTCTGC

Downstream 100 bases:

>100_bases
TCGCCGATTCTGATGCATGATGCCGTCTTATGGACGGCATTTTTTGCGCGCTGTTCTTCCACCCTTTCTCTGCAATGCGT
AATTCCCACACAATTCCTGT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAVTPVSRANHVVAVALSKIFVNSKIKCTQKANFPKNRYPESKELLMSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRK
NENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIA
FVKDPDGYMIELIQNKQAHAGLEG

Sequences:

>Translated_184_residues
MAVTPVSRANHVVAVALSKIFVNSKIKCTQKANFPKNRYPESKELLMSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRK
NENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIA
FVKDPDGYMIELIQNKQAHAGLEG
>Mature_183_residues
AVTPVSRANHVVAVALSKIFVNSKIKCTQKANFPKNRYPESKELLMSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKN
ENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAF
VKDPDGYMIELIQNKQAHAGLEG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=166, Percent_Identity=37.3493975903614, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=68.1818181818182, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17552228, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=136, Percent_Identity=42.6470588235294, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=39.7260273972603, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920404, Length=141, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 20287; Mature: 20156

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTPVSRANHVVAVALSKIFVNSKIKCTQKANFPKNRYPESKELLMSNHRILHTMLRVG
CCCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHC
DLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
YGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHA
EEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
GLEG
CCCC
>Mature Secondary Structure 
AVTPVSRANHVVAVALSKIFVNSKIKCTQKANFPKNRYPESKELLMSNHRILHTMLRVG
CCCCCCCCCEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHC
DLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
YGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHA
EEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
GLEG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]