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The map label for this gene is folC [H]

Identifier: 227081175

GI number: 227081175

Start: 1029339

End: 1030604

Strand: Direct

Name: folC [H]

Synonym: VCM66_0957

Alternate gene names: 227081175

Gene position: 1029339-1030604 (Clockwise)

Preceding gene: 227081174

Following gene: 227081176

Centisome position: 35.59

GC content: 50.95

Gene sequence:

>1266_bases
ATGACTCAAAAGCCTGTTCCTCAAGCCACATCTCCACTCGCGGTGTGGCTTGATTATTTAGCCCACATACACACTTCCGC
CATTGATCTCGGCCTAGAACGTGTTCAAGCCGTCGCTCAAAAAGCCAAACTCACCAAACCAGCGCCCACCGTTATCACGG
TGGCGGGCACCAATGGTAAAGGTTCCACCTGTGCATTAATGGAAGCGATTCTACTGCAAGCGGGTTATTCCGTTGGCGTG
TACAGTTCTCCGCACTTGATTCGCTATAACGAGCGTGTACGCATCAATGGCCAAGATCTTGCCGATGAGATGCACACCCA
AGCGTTTGCGCATATTGAGCAAGAGCGCGGTGACATAAGCTTAAGCCTATTTGAATTCGGTACACTCGCCGCTCTTTATC
TTTTTGAGCAGCAGAAAGTCGATGTGTTGCTGCTTGAAGTGGGGTTGGGTGGCCGTTTAGATGCGACCAACGTTGTCGAT
CACGATGTCAGTGTCATCACCAGCTTAGCGCTGGATCATACCGATTGGCTTGGCAATGACATCGCTGTGATTGGTTATGA
GAAAGCGGGCATCTTTCGTTCAGGAAAGCCTGCGATTTGTGGGCAGCCTAAGCCACCCGCAACCGTTGCTGCGCATGCCG
ATGATATCGGCGCTAAACTGCATCAGGTGGAAATCCAATTTAGTTATGAGAAAACCTCAGCAACGACTTGGAATTGGCGT
AGTGGCCGCTTTGAATTGACCGATCTTCCTATCCCATCTCTGCCGTTACCGAATGCGGCAACGGCTTTAATGGCGCTGGG
TTGCTCTGGGCTTGCCATCAGCGATATTAATATCGTGGATGGGCTACGGAATGCCCAATTAGCGGGACGAATGCAGGTGA
TTAGCCATCAACCTACGATAGTGCTTGATGTCGCACATAACCCGCACTCAGCCCATTATCTGGTTCAGCAACTGACACAA
CGCTACCCAGAGCGCACCATTCATCTGGTGTTTGGGATGTTGCATGATAAAGATATTGCTTCAACGGTGGTCGAGTTAAG
CCCGGCGGTGACACATTGGTATCCCGCTTCGTTGTCTGGCCCTCGTGCGGCTACTGCGGCGCAGCTCTGTCAGTATTTAC
CTGCGGATATTACTCAATATGCCAATCCTGTGTCGGCATTTGAAGCCGCGTTGACACAGGCTGGGGCACAAGATGTCATT
GTAGTTGCGGGCTCTTTCCATACGGTAGGTGAAGTGCTTGAACATTGGCAAAAGAGAGGGGAATAA

Upstream 100 bases:

>100_bases
ACGACGCGCCAAATGAAGTGCCTTATGCGGTACCAGAAGCCAATAAAAAAGGGTAAAGTAAGCGCTAACTAAACCAACGG
ATTCTATGGTTGTACGTTAG

Downstream 100 bases:

>100_bases
ATGGCCAGTAAATTTCAAAGCCGTTTAGTTGGCACCATAGTGTTGGTCGCCATCGGCGTGATTGTGCTGCCCGATGTGCT
TGATGGTAAGAAAACGCATT

Product: folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGKGSTCALMEAILLQAGYSVGV
YSSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDISLSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVD
HDVSVITSLALDHTDWLGNDIAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWR
SGRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTIVLDVAHNPHSAHYLVQQLTQ
RYPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSGPRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVI
VVAGSFHTVGEVLEHWQKRGE

Sequences:

>Translated_421_residues
MTQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGKGSTCALMEAILLQAGYSVGV
YSSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDISLSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVD
HDVSVITSLALDHTDWLGNDIAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWR
SGRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTIVLDVAHNPHSAHYLVQQLTQ
RYPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSGPRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVI
VVAGSFHTVGEVLEHWQKRGE
>Mature_420_residues
TQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGKGSTCALMEAILLQAGYSVGVY
SSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDISLSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVDH
DVSVITSLALDHTDWLGNDIAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWRS
GRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTIVLDVAHNPHSAHYLVQQLTQR
YPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSGPRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVIV
VAGSFHTVGEVLEHWQKRGE

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=342, Percent_Identity=31.5789473684211, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=31.5789473684211, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1788654, Length=414, Percent_Identity=53.8647342995169, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17553148, Length=462, Percent_Identity=28.1385281385281, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=462, Percent_Identity=28.1385281385281, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=462, Percent_Identity=28.1385281385281, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=337, Percent_Identity=29.3768545994065, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6324815, Length=287, Percent_Identity=28.9198606271777, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24641571, Length=277, Percent_Identity=33.9350180505415, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24581568, Length=203, Percent_Identity=36.4532019704434, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45473; Mature: 45342

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMEAILLQAGYSVGVYSSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDIS
CCHHHHHHHHHHHCCCCEEECCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHCCCCEE
LSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVDHDVSVITSLALDHTDWLGND
EEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWR
EEEEEEECCCCEECCCCCCCCCCCCCCEEEECCHHHCCEEEEEEEEEEEECCCCCEECCC
SGRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTI
CCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHCCCCHHHHHHHHEECCCCEE
VLDVAHNPHSAHYLVQQLTQRYPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSG
EEEECCCCCHHHHHHHHHHHHCCHHEEHHHHHHHCCHHHHHHHHHCCCHHHEEECCCCCC
PRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVIVVAGSFHTVGEVLEHWQKRG
CCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure 
TQKPVPQATSPLAVWLDYLAHIHTSAIDLGLERVQAVAQKAKLTKPAPTVITVAGTNGK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMEAILLQAGYSVGVYSSPHLIRYNERVRINGQDLADEMHTQAFAHIEQERGDIS
CCHHHHHHHHHHHCCCCEEECCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHCCCCEE
LSLFEFGTLAALYLFEQQKVDVLLLEVGLGGRLDATNVVDHDVSVITSLALDHTDWLGND
EEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
IAVIGYEKAGIFRSGKPAICGQPKPPATVAAHADDIGAKLHQVEIQFSYEKTSATTWNWR
EEEEEEECCCCEECCCCCCCCCCCCCCEEEECCHHHCCEEEEEEEEEEEECCCCCEECCC
SGRFELTDLPIPSLPLPNAATALMALGCSGLAISDINIVDGLRNAQLAGRMQVISHQPTI
CCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHCCCCHHHHHHHHEECCCCEE
VLDVAHNPHSAHYLVQQLTQRYPERTIHLVFGMLHDKDIASTVVELSPAVTHWYPASLSG
EEEECCCCCHHHHHHHHHHHHCCHHEEHHHHHHHCCHHHHHHHHHCCCHHHEEECCCCCC
PRAATAAQLCQYLPADITQYANPVSAFEAALTQAGAQDVIVVAGSFHTVGEVLEHWQKRG
CCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]