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The map label for this gene is rfaH [H]

Identifier: 227081164

GI number: 227081164

Start: 1011751

End: 1012248

Strand: Direct

Name: rfaH [H]

Synonym: VCM66_0946

Alternate gene names: 227081164

Gene position: 1011751-1012248 (Clockwise)

Preceding gene: 227081163

Following gene: 227081169

Centisome position: 34.98

GC content: 45.18

Gene sequence:

>498_bases
ATGAAACGTTGGTATCTACTTTACTGCAAACGAGGCGAGCAGCAGCGCGCGAAGATGCACTTAGAAAATCAAAGTGTTGA
ATGCTTCTACCCCGAAGTGTGCGTTGAAAAAATTCTACGTGGTAAGCGGCAAATGGTTCAGGAGCCGTTATTCCCTTCTT
ATATGTTTGTGCGTTTCGATTTCGAAAATGGACCTTCATTTACGACCGTACGTTCTACGCGTGGTGTCGTAGATTTTGTC
CGCTTGGGGCCTCATCCACGTGAACTGCAAGGCGATTTGATTTATCAATTGAAACAGCTTGATTGTGAGCAATTGAAGCA
CGCCACCAAACAATTGCCAGAAAAAGGGCAAACCGTTCGAGTCGCAAGAGGTCAGTTTGCGGGCATTGAAGCGATTTATT
TAGAGCCGGATGGTGATACTCGCTCGATTATGCTGGTGAAGATGATCAGTCAGCAAGTACCAATGAGTATTGAAAATACC
GATTGGGAAGTCACCTAA

Upstream 100 bases:

>100_bases
ATCAGGTGCGGGGGACTCAACGCTTTTTACAAGAGTGCATTTCATATTTGCTCTGTGGGTGTGGTAGCATGCCGAAAAAG
GTTGGCATAACAGAATATAA

Downstream 100 bases:

>100_bases
TCTTATCTTTACCGTCTTGATTTTTCTGTTGATGAATCATCGGGTGATGAAAGAATTAAAAGCCAGAATGAGTCATCACT
CTGGCTTTTTTAATCACTAT

Product: transcriptional activator RfaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKRWYLLYCKRGEQQRAKMHLENQSVECFYPEVCVEKILRGKRQMVQEPLFPSYMFVRFDFENGPSFTTVRSTRGVVDFV
RLGPHPRELQGDLIYQLKQLDCEQLKHATKQLPEKGQTVRVARGQFAGIEAIYLEPDGDTRSIMLVKMISQQVPMSIENT
DWEVT

Sequences:

>Translated_165_residues
MKRWYLLYCKRGEQQRAKMHLENQSVECFYPEVCVEKILRGKRQMVQEPLFPSYMFVRFDFENGPSFTTVRSTRGVVDFV
RLGPHPRELQGDLIYQLKQLDCEQLKHATKQLPEKGQTVRVARGQFAGIEAIYLEPDGDTRSIMLVKMISQQVPMSIENT
DWEVT
>Mature_165_residues
MKRWYLLYCKRGEQQRAKMHLENQSVECFYPEVCVEKILRGKRQMVQEPLFPSYMFVRFDFENGPSFTTVRSTRGVVDFV
RLGPHPRELQGDLIYQLKQLDCEQLKHATKQLPEKGQTVRVARGQFAGIEAIYLEPDGDTRSIMLVKMISQQVPMSIENT
DWEVT

Specific function: Transcriptional activator of hemolysin synthesis and secretion, also required for expression of sex factor and lipopolysaccharide biosynthesis genes. Transcriptional activator of rfaD/htrM and htrL [H]

COG id: COG0250

COG function: function code K; Transcription antiterminator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790276, Length=162, Percent_Identity=44.4444444444444, Blast_Score=149, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005824
- InterPro:   IPR010215
- InterPro:   IPR006645
- InterPro:   IPR014722 [H]

Pfam domain/function: PF02357 NusG [H]

EC number: NA

Molecular weight: Translated: 19334; Mature: 19334

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWYLLYCKRGEQQRAKMHLENQSVECFYPEVCVEKILRGKRQMVQEPLFPSYMFVRFD
CCCEEEEEECCCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
FENGPSFTTVRSTRGVVDFVRLGPHPRELQGDLIYQLKQLDCEQLKHATKQLPEKGQTVR
CCCCCCEEEEECCCCHHHHHHCCCCCHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEE
VARGQFAGIEAIYLEPDGDTRSIMLVKMISQQVPMSIENTDWEVT
EECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEC
>Mature Secondary Structure
MKRWYLLYCKRGEQQRAKMHLENQSVECFYPEVCVEKILRGKRQMVQEPLFPSYMFVRFD
CCCEEEEEECCCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
FENGPSFTTVRSTRGVVDFVRLGPHPRELQGDLIYQLKQLDCEQLKHATKQLPEKGQTVR
CCCCCCEEEEECCCCHHHHHHCCCCCHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEE
VARGQFAGIEAIYLEPDGDTRSIMLVKMISQQVPMSIENTDWEVT
EECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]