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The map label for this gene is toxR

Identifier: 227081158

GI number: 227081158

Start: 1004575

End: 1005459

Strand: Reverse

Name: toxR

Synonym: VCM66_0940

Alternate gene names: 227081158

Gene position: 1005459-1004575 (Counterclockwise)

Preceding gene: 227081162

Following gene: 227081157

Centisome position: 34.76

GC content: 45.42

Gene sequence:

>885_bases
ATGTTCGGATTAGGACACAACTCAAAAGAGATATCGATGAGTCATATTGGTACTAAATTCATTCTTGCTGAAAAATTTAC
CTTTGATCCCCTAAGCAATACTCTGATTGACAAAGAAGATAGTGAAGAGATCATTCGATTAGGCAGCAACGAAAGCCGAA
TTCTTTGGCTGCTGGCCCAACGTCCAAACGAGGTGATTTCTCGCAATGATTTGCATGACTTTGTTTGGCGAGAGCAAGGT
TTTGAAGTCGATGATTCCAGCTTAACCCAAGCCATTTCGACTCTGCGCAAAATGCTCAAAGATTCGACAAAGTCCCCACA
ATACGTCAAAACGGTTCCGAAACGCGGTTACCAATTGATCGCCCGAGTGGAAACGGTTGAAGAAGAGATGGCTCGCGAAA
GCGAAGCTGCTCATGACATCTCTCAGCCAGAATCTGTCAATGAATACGCAGAGTCAAGCAGTGTGCCTTCATCAGCCACT
GTAGTGAACACACCGCAGCCAGCCAATGTTGTGACGAATAAATCGGCTCCAAACTTGGGGAATCGACTGCTTATTCTGAT
AGCGGTCTTACTTCCCCTCGCAGTATTACTGCTCACTAACCCGAGCCAAACCAGCTTTAAACCCCTAACGGTTGTCGATG
GCGTAGCCGTCAATATGCCGAATAACCACCCTGATCTTTCAAACTGGCTACCGTCAATCGAACTGTGCGTTAAAAAATAC
AATGAAAAGCATACTGGTGGGCTCAAGCCGATAGAAGTCATTGCCACAGGTGGACAAAATAACCAGTTAACGCTGAATTA
CATTCACAGCCCTGAAGTTTCAGGGGAAAACATAACCTTACGCATCGTTGCTAACCCTAACGATGCCATCAAAGTGTGTG
AGTAG

Upstream 100 bases:

>100_bases
TTGATCGAGATTGGATTATTCTAAGTCTGCATTTTTATCAAAGAAGATAAAAAAACCAGTAAAGTCTGAGTGTTGGGACA
GGGAGATACTGGGACATTAG

Downstream 100 bases:

>100_bases
GATCTTGCTATGCAAAATAGACACATCGCCATGGGTATTCTTCTGCTCTCTCTGCTGCTCAGTAGCTGGTTATATTGGGG
GAGTGACTTCAAGCTTGAGC

Product: cholera toxin transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MFGLGHNSKEISMSHIGTKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQRPNEVISRNDLHDFVWREQG
FEVDDSSLTQAISTLRKMLKDSTKSPQYVKTVPKRGYQLIARVETVEEEMARESEAAHDISQPESVNEYAESSSVPSSAT
VVNTPQPANVVTNKSAPNLGNRLLILIAVLLPLAVLLLTNPSQTSFKPLTVVDGVAVNMPNNHPDLSNWLPSIELCVKKY
NEKHTGGLKPIEVIATGGQNNQLTLNYIHSPEVSGENITLRIVANPNDAIKVCE

Sequences:

>Translated_294_residues
MFGLGHNSKEISMSHIGTKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQRPNEVISRNDLHDFVWREQG
FEVDDSSLTQAISTLRKMLKDSTKSPQYVKTVPKRGYQLIARVETVEEEMARESEAAHDISQPESVNEYAESSSVPSSAT
VVNTPQPANVVTNKSAPNLGNRLLILIAVLLPLAVLLLTNPSQTSFKPLTVVDGVAVNMPNNHPDLSNWLPSIELCVKKY
NEKHTGGLKPIEVIATGGQNNQLTLNYIHSPEVSGENITLRIVANPNDAIKVCE
>Mature_294_residues
MFGLGHNSKEISMSHIGTKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQRPNEVISRNDLHDFVWREQG
FEVDDSSLTQAISTLRKMLKDSTKSPQYVKTVPKRGYQLIARVETVEEEMARESEAAHDISQPESVNEYAESSSVPSSAT
VVNTPQPANVVTNKSAPNLGNRLLILIAVLLPLAVLLLTNPSQTSFKPLTVVDGVAVNMPNNHPDLSNWLPSIELCVKKY
NEKHTGGLKPIEVIATGGQNNQLTLNYIHSPEVSGENITLRIVANPNDAIKVCE

Specific function: This transcription activator controls cholera toxin, pilus colonization factor and outer membrane protein expression in V.cholerae. It binds to the 5'-TTTTGAT-3' tandemly repeated DNA sequence in the cholera toxin promoter region. ToxS interacts with the

COG id: COG3710

COG function: function code K; DNA-binding winged-HTH domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790576, Length=116, Percent_Identity=39.6551724137931, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOXR_VIBCH (P15795)

Other databases:

- EMBL:   M21249
- EMBL:   AE003852
- EMBL:   M58033
- EMBL:   M62761
- PIR:   A25970
- PIR:   E82257
- RefSeq:   NP_230630.1
- ProteinModelPortal:   P15795
- GeneID:   2614237
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0984
- TIGR:   VC_0984
- HOGENOM:   HBG625927
- OMA:   FVKTVPK
- ProtClustDB:   CLSK874172
- GO:   GO:0005622
- GO:   GO:0006350
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- SMART:   SM00862

Pfam domain/function: PF00486 Trans_reg_C; SSF46894 Bipartite_resp_reg_C-effector

EC number: NA

Molecular weight: Translated: 32506; Mature: 32506

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2606197c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLGHNSKEISMSHIGTKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQ
CCCCCCCCCEEEHHHCCEEEEEEECCCCCCHHHHHCCCCCCHHHHHCCCCCCEEEEEEEC
RPNEVISRNDLHDFVWREQGFEVDDSSLTQAISTLRKMLKDSTKSPQYVKTVPKRGYQLI
CCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ARVETVEEEMARESEAAHDISQPESVNEYAESSSVPSSATVVNTPQPANVVTNKSAPNLG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCC
NRLLILIAVLLPLAVLLLTNPSQTSFKPLTVVDGVAVNMPNNHPDLSNWLPSIELCVKKY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHH
NEKHTGGLKPIEVIATGGQNNQLTLNYIHSPEVSGENITLRIVANPNDAIKVCE
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEECH
>Mature Secondary Structure
MFGLGHNSKEISMSHIGTKFILAEKFTFDPLSNTLIDKEDSEEIIRLGSNESRILWLLAQ
CCCCCCCCCEEEHHHCCEEEEEEECCCCCCHHHHHCCCCCCHHHHHCCCCCCEEEEEEEC
RPNEVISRNDLHDFVWREQGFEVDDSSLTQAISTLRKMLKDSTKSPQYVKTVPKRGYQLI
CCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ARVETVEEEMARESEAAHDISQPESVNEYAESSSVPSSATVVNTPQPANVVTNKSAPNLG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCCC
NRLLILIAVLLPLAVLLLTNPSQTSFKPLTVVDGVAVNMPNNHPDLSNWLPSIELCVKKY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHH
NEKHTGGLKPIEVIATGGQNNQLTLNYIHSPEVSGENITLRIVANPNDAIKVCE
CCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3802195; 10952301; 2124707; 1898871; 1400230