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The map label for this gene is ptsI [H]

Identifier: 227081139

GI number: 227081139

Start: 987194

End: 988915

Strand: Reverse

Name: ptsI [H]

Synonym: VCM66_0921

Alternate gene names: 227081139

Gene position: 988915-987194 (Counterclockwise)

Preceding gene: 227081140

Following gene: 227081138

Centisome position: 34.19

GC content: 46.11

Gene sequence:

>1722_bases
ATGATTTCAGGCATTCTAGCATCTCCAGGTATTGCTATTGGTAAAGCATTACTTCTTCAAGAAGATGAAATTGTCCTAAA
CACAAACACCATTTCTGACGATCAAGTAGAAGCCGAAGTTGAGCGCTTTTACACTGCGCGTGACAAATCTTCTGCGCAGT
TAGAAGTGATCAAACAAAAAGCACTTGAAACTTTTGGTGAAGAAAAAGAAGCCATCTTTGAAGGCCATATCATGTTGCTT
GAAGATGAAGAGCTAGAAGAAGAGATTTTAGCCCTCATCAAAAAAGAAAAAATGTCTGCCGATAACGCAATTCATACCGT
TATCGAAGAGCAAGCCACTGCGCTTGAATCTTTAGACGATGAATATCTCAAAGAACGTGCAACAGATATCCGTGATATCG
GTACACGTTTTGTAAAAAATGCTCTGGGCATGCACATCGTATCGCTGAGCGAAATCGATCAAGAAGTTGTCCTTGTTGCT
TACGATTTAACGCCTTCTGAAACTGCGCAGATTAACCTGAACTACGTTCTCGGTTTTGCATGTGATATTGGCGGCCGTAC
CTCTCACACTTCTATCATGGCTCGCTCTCTAGAGCTACCAGCGATTGTCGGTACTAACGACATCACTAAGAAAGTGAAAA
ATGGCGACACATTGATTCTGGACGCAATGAACAACAAGATCATTGTGAATCCAACTCAAGCCCAAATTGAAGAAGCCAAA
GCGGTTAAAGCGGCTTTCCTAGCGGAAAAAGAAGAGCTCGCTAAGCTGAAAGATCTTCCAGCAGAAACGCTAGACGGCCA
TCGCGTTGAAGTGTGTGGCAACATCGGTACCGTGAAAGACTGTGACGGTATCATCCGTAACGGTGGTGAAGGTGTCGGCC
TATACCGTACTGAATTCCTGTTTATGGATCGTGATGCCCTGCCAACAGAAGAAGAACAGTACCAAGCCTACAAAGAAGTC
GCGGAAGCGATGAATGGCCAAGCGGTTATCATCCGCACCATGGACATCGGCGGCGACAAAGACCTGCCGTATATGGATCT
GCCAAAAGAGATGAACCCATTCTTGGGTTGGCGTGCCGTTCGTATCAGCTTAGATCGCCGTGAAATTCTACGTGACCAGC
TACGCGGTATTCTGCGTGCCTCTGCACACGGCAAACTTCGCGTTATGTTCCCAATGATCATTTCAGTAGAAGAAATTCGT
GAACTGAAAAATGCGATTGAAGAGTACAAAGCTGAACTGCGCACCGAAGGTCATGCTTTTGATGAAAACATCGAAATCGG
TGTGATGGTAGAAACGCCAGCTGCAGCAGCGATTGCTCATCACTTGGCAAAAGAAGTCTCTTTCTTCTCTATCGGTACCA
ACGATTTAACTCAGTATACCCTAGCGGTAGACCGTGGTAACGAGATGATCTCTCACCTCTACAACCCACTCTCTCCAGCG
GTTCTGACCGTGATCAAGCAGGTGATTGATGCTTCTCACGCAGAAGGCAAATGGACTGGTATGTGTGGTGAATTAGCGGG
TGACGAGCGTGCAACGCTACTCCTACTCGGTATGGGTCTGGATGAGTTCTCAATGAGCGGCATCTCGATTCCAAAAGTGA
AGAAAGTGATCCGTAACGCCAATTATGCGGAAATCAAAGCGATGGCTGAAGAGGCGCTTGCCCTGCCAACGGCTGCAGAA
ATTGAAGCTTGCGTAGATAAATTCATCGCAGCGAAAGCGTAA

Upstream 100 bases:

>100_bases
CAAAATGGCTTTGTTGGAAATAACCCGAGAAATACGCTAAACGTTACGGTTAGCGATTGTCCACTCTCCCGTTTACAGTT
GACCAGTTTAAGGTAAGGCT

Downstream 100 bases:

>100_bases
TTATCGGTTGATACCAAGGAGATAGGCGATGAAAATAGTCGTCTATCTTTAGCGATTGGTATACTATACTTCGACAGAAC
AAATTTAAAACGTTAGGAGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDDEYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVA
YDLTPSETAQINLNYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYQAYKEV
AEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIR
ELKNAIEEYKAELRTEGHAFDENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNANYAEIKAMAEEALALPTAAE
IEACVDKFIAAKA

Sequences:

>Translated_573_residues
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDDEYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVA
YDLTPSETAQINLNYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYQAYKEV
AEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIR
ELKNAIEEYKAELRTEGHAFDENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNANYAEIKAMAEEALALPTAAE
IEACVDKFIAAKA
>Mature_573_residues
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQKALETFGEEKEAIFEGHIMLL
EDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDDEYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVA
YDLTPSETAQINLNYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYQAYKEV
AEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAVRISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIR
ELKNAIEEYKAELRTEGHAFDENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNANYAEIKAMAEEALALPTAAE
IEACVDKFIAAKA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=572, Percent_Identity=74.8251748251748, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=579, Percent_Identity=37.3056994818653, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI48994992, Length=503, Percent_Identity=38.9662027833002, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=33.160621761658, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1787994, Length=385, Percent_Identity=26.7532467532467, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI226510935, Length=214, Percent_Identity=28.0373831775701, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63133; Mature: 63133

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQK
CCCCCCCCCCHHHHHHHEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDD
HHHHHCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
EYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVAYDLTPSETAQINLNYVLGFA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCEEEEEEEECCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
EECCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEE
FMDRDALPTEEEQYQAYKEVAEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHCCEEEE
RISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIRELKNAIEEYKAELRTEGHAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNA
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NYAEIKAMAEEALALPTAAEIEACVDKFIAAKA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISGILASPGIAIGKALLLQEDEIVLNTNTISDDQVEAEVERFYTARDKSSAQLEVIKQK
CCCCCCCCCCHHHHHHHEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALETFGEEKEAIFEGHIMLLEDEELEEEILALIKKEKMSADNAIHTVIEEQATALESLDD
HHHHHCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
EYLKERATDIRDIGTRFVKNALGMHIVSLSEIDQEVVLVAYDLTPSETAQINLNYVLGFA
HHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCEEEEEEEECCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKKVKNGDTLILDAMNNKIIVNPTQAQIEEAK
EECCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
AVKAAFLAEKEELAKLKDLPAETLDGHRVEVCGNIGTVKDCDGIIRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEE
FMDRDALPTEEEQYQAYKEVAEAMNGQAVIIRTMDIGGDKDLPYMDLPKEMNPFLGWRAV
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHCCEEEE
RISLDRREILRDQLRGILRASAHGKLRVMFPMIISVEEIRELKNAIEEYKAELRTEGHAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DENIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCCEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLTVIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSGISIPKVKKVIRNA
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHCCCCCCHHHHHHHHHCC
NYAEIKAMAEEALALPTAAEIEACVDKFIAAKA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]