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The map label for this gene is dacA-1 [H]

Identifier: 227081121

GI number: 227081121

Start: 968460

End: 969635

Strand: Reverse

Name: dacA-1 [H]

Synonym: VCM66_0903

Alternate gene names: 227081121

Gene position: 969635-968460 (Counterclockwise)

Preceding gene: 227081122

Following gene: 227081119

Centisome position: 33.52

GC content: 47.02

Gene sequence:

>1176_bases
ATGAAAAAATCAATCCTCAAATCTGTGTTAGCGTCTTCTATTACGCTTTCTATCACGCTAAGCAGCACTGCTTTCGCCTC
TCCTATCGTGACACCAGACGCACCACAAATTGCCGCTAAAGGCTATGTACTGATGGATTACCATTCAGGAAAAGTGTTGG
CTGAAAAAGAGATGGATACCAAACTCTCTCCTGCCAGCCTAACCAAGATGATGACCAGTTACGTGATCGGTCAAGAAGTA
AAGCGTGGCAATATCTCGCTGAATGACGATGTAGTGATCAGCAAAAATGCTTGGGCGAAAAACTTCCCTGATTCTTCGAA
AATGTTCGTTGAAGTCGGTACCACGGTTAAGGTCAGCGATCTCAACCGCGGCATCATCATCCAATCGGGTAATGATGCGT
GTGTAGCGATGGCAGAGCACGTCGCAGGGACGGAAGATGCGTTTGTTGACCTGATGAACGCTTGGGCAAGCTCACTTGGG
ATGAAGAATTCGCACTTCACTAACTCTCACGGTTTGGACGATCCTAATCTTTACTCTACGCCATACGATCTGGCTCTACT
TGGCCAAGCTCTGATCCGTGATGTGCCTGAAGAGTACGCGATCTACTCCGAGCAAAAATTCACCTACAACGGCATCACTC
AATACAACCGTAACGGTTTGCTTTGGGATAAAAGCATGAATGTGGACGGCATTAAAACCGGACATACGAGTGGTGCTGGC
TATAACCTCGTGAGCTCTGCTACAGAAGGCAACATGCGCTTGGTTGCGGTGGTGATGGGTACTGACAACGAAAACGCGCG
TAAAGCGGAAAGTAAGAAGCTATTGAGCTACGGTTTCCGCTTCTTTGAAACCATGGCACCACACAAAGCGGGTGAGACTT
TCGTAAATGAAACCATCTGGATGGGCGATAAAGACACGATTGCACTCGGTGTTGATAAAGATACTTACGTAACTTTGCCA
CGCGGACAGGCCAAAGATCTCACCGCCAGCTTTGTACTTGAAAAACAATTGAAAGCACCGCTGAAGAAAGGCGATGTCGT
CGGCACACTTTACTATCAATTAGCTGGTAACGATATTGCTCAATACCCATTGCTCGCACTGGAAGATGTGCAAGAAGGCA
GCCTATTTAGCCGCCTATGGGATTACTTGGTTCTGCTGTTCAAAAGCTGGTTTTAA

Upstream 100 bases:

>100_bases
GCTAAATGACCACTCAAACCTGACCTGAGTCTATCTGGTCTGGTTTGAGACGTTATTTTTGTTAAGATTACGGACACTTA
AGTAGAACTAAGCATTCACC

Downstream 100 bases:

>100_bases
ACCGTTCAGCCAGTAAAGCCAATACTGTCTAAAGAGATTGATCTCCCCAAAAGCCGCTTCATGCGGCTTTTTTGTGAGCT
TGAGATCACGCCCGAATGGC

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDTKLSPASLTKMMTSYVIGQEV
KRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSDLNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLG
MKNSHFTNSHGLDDPNLYSTPYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETMAPHKAGETFVNETIWMGDKDTIALGVDKDTYVTLP
RGQAKDLTASFVLEKQLKAPLKKGDVVGTLYYQLAGNDIAQYPLLALEDVQEGSLFSRLWDYLVLLFKSWF

Sequences:

>Translated_391_residues
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDTKLSPASLTKMMTSYVIGQEV
KRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSDLNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLG
MKNSHFTNSHGLDDPNLYSTPYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETMAPHKAGETFVNETIWMGDKDTIALGVDKDTYVTLP
RGQAKDLTASFVLEKQLKAPLKKGDVVGTLYYQLAGNDIAQYPLLALEDVQEGSLFSRLWDYLVLLFKSWF
>Mature_391_residues
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDTKLSPASLTKMMTSYVIGQEV
KRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSDLNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLG
MKNSHFTNSHGLDDPNLYSTPYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETMAPHKAGETFVNETIWMGDKDTIALGVDKDTYVTLP
RGQAKDLTASFVLEKQLKAPLKKGDVVGTLYYQLAGNDIAQYPLLALEDVQEGSLFSRLWDYLVLLFKSWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=390, Percent_Identity=55.8974358974359, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1787062, Length=392, Percent_Identity=53.5714285714286, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI87082022, Length=384, Percent_Identity=44.53125, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI87082054, Length=255, Percent_Identity=26.2745098039216, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42907; Mature: 42907

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDT
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCC
KLSPASLTKMMTSYVIGQEVKRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCEEEEEC
LNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLGMKNSHFTNSHGLDDPNLYST
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
CHHHHHHHHHHHHHCCHHHHEECCCCEEECCCEEECCCCEEEECCCCCCCEECCCCCCCC
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETMAPHKAGETFVNETIW
CEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEE
MGDKDTIALGVDKDTYVTLPRGQAKDLTASFVLEKQLKAPLKKGDVVGTLYYQLAGNDIA
ECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCEEEEEEEEECCCCHH
QYPLLALEDVQEGSLFSRLWDYLVLLFKSWF
HCCCCEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKSILKSVLASSITLSITLSSTAFASPIVTPDAPQIAAKGYVLMDYHSGKVLAEKEMDT
CCHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHCCEEEEEECCCCEEEECCCCC
KLSPASLTKMMTSYVIGQEVKRGNISLNDDVVISKNAWAKNFPDSSKMFVEVGTTVKVSD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEECCEEEEEC
LNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASSLGMKNSHFTNSHGLDDPNLYST
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PYDLALLGQALIRDVPEEYAIYSEQKFTYNGITQYNRNGLLWDKSMNVDGIKTGHTSGAG
CHHHHHHHHHHHHHCCHHHHEECCCCEEECCCEEECCCCEEEECCCCCCCEECCCCCCCC
YNLVSSATEGNMRLVAVVMGTDNENARKAESKKLLSYGFRFFETMAPHKAGETFVNETIW
CEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEE
MGDKDTIALGVDKDTYVTLPRGQAKDLTASFVLEKQLKAPLKKGDVVGTLYYQLAGNDIA
ECCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHCCCEEEEEEEEECCCCHH
QYPLLALEDVQEGSLFSRLWDYLVLLFKSWF
HCCCCEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]