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The map label for this gene is yneF [H]

Identifier: 227081075

GI number: 227081075

Start: 916255

End: 917829

Strand: Reverse

Name: yneF [H]

Synonym: VCM66_0857

Alternate gene names: 227081075

Gene position: 917829-916255 (Counterclockwise)

Preceding gene: 227081076

Following gene: 227081074

Centisome position: 31.73

GC content: 47.49

Gene sequence:

>1575_bases
TTGTTGTACATGGGCGTTCTAGAACAAGATATCCAAGCAGAGCTTCAAAAACTGAAATCTCAGTTAGAACAAGTACGTTT
GACACAAAGGGATACTTCGTTCAAATTTAACCGAGAGCAGCAAGTTCTTAAACGAATGGTCACTTCCTTGGCTACGACTT
GCCGCGGCAGTAGCTCTCGACTAAACCAAAGTTTAGAAGAACTCTGTTTTGCATTAGAACAACAAAAAGACATCAGTACG
CTGATCCCGAAATTAGCCGTACTGGAACGCATGTTGAAACAACAGTCATTGGCCATGGACAAACAAACGAGTCATCTGGA
TAGCCAGCTACAGCACAGTGGTGAAACGCTACTGCGCGTCCCCGGCTTACCCGCCAAGATCAAACGCGATCTTCGAGATC
TGCTGAGTTTTAGCGCCGCACAGCCGCTGGAAAAAACTCAGCAAGCTTTGCGTTTGCTGGAAATTTATGAACGCGCGGTC
AAAATTCAATGTGCCAATCCCAATTTGGCGCTCAATGAGCTTACTCAACAAGCGGATCGAGAACTGCTGCATCGACTTGC
CGTCGATTTACAACACTTGATTACTGAGTTGGATTTCAATGGTGAATCGGGTGATCAACTGACCGATATCCGAACCAAGC
TTCTGCTTGGCGTCAATACCCATACCCTGCTTGAGCTCACCCTTGAAGTGCTGCGTTTAGTCGTAGATGGAAGCCATGCT
GAGCGCAAAGCAGCCGAAGCCTTTCTCGAGCAAGTGAATCTCTCGATTGGCAATAACCTAAAAAGCGCGACTCAAAATCT
TGAGCAAAGCCAGAGTTACATGGCACACCGCCAAGAGATGAATGGCGAGCTGAGCAGTTTAGTGGCTCGTAGCCAGACCG
CGCTCAAAGAACAGACGGAGCTAGAACAGCTCAAGCTGAAAATGGCGCCTTTACTGCGCGAGATGTCTTCACTTTCTGAA
CGCTTACTCTTAGCGGAGCAACGTGAGCAAGCCCTACTTGAGCGTATGCGCTATAGCAAAGATCAGATGGAAGCCCTGTC
TGATTTGGCACAAGATTATCGTCGCCGCCTTGAAGATCAAGCATTGCGCGCGCAACTCGATCCTCTGACCAAAGTGTACA
ACCGCAGCAGCTTTACTGAGCGACTTGAACATGAGTATCGCCGCTGGATCCGTACGCAACACAATTTGCGGGTAGTGCTG
TTTGATATTGATAAATTCAAATCGATCAACGACAGCTTTGGCTATACCGCAGGCGATAAGGCCTTAAGTATCATTGCTCG
CACCATCAAAAAAGAATTACGAGACAGTGACACCGTGGCTCGCTTCTCTGGTGAAGAGTTCATTCTGTTACTGCCTGAAC
GCTCCGATAATGAGAGTTACCAGATTATTCACCAGATCCAGCTCAACGTGTCGAAACTACCGTTCAAGTTCCGCGATAAG
AGCCTAACCATCACGCTGTCTGCGGCGAGTATCCGTTTCATGGATTCAGATACCCCCGAAACGGTTCTTGATCGTTTAAA
TCTGACGCTAAGTGAAGCCAAACATATCGGTCCAAGTCAGTTAGTTTGGAAATAA

Upstream 100 bases:

>100_bases
TGCTGCCCAGTCGGCATAAACACTGAGTGACGCCAGCAAAATATTTGTTATTATCGACAAATCAATAAATAGGATCAGGA
TTGATCCTCAACTATCCAGT

Downstream 100 bases:

>100_bases
CTGATTAATATTTAAGCTAATTAACTATTAATTTGTGCTCAATTTCACAATTACCCCCTCACTCTATTTTCTTACCTGAC
TAGATACCTCTAGATTGAAG

Product: GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLVWK

Sequences:

>Translated_524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLVWK
>Mature_524_residues
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSRLNQSLEELCFALEQQKDIST
LIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRVPGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAV
KIQCANPNLALNELTQQADRELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTELEQLKLKMAPLLREMSSLSE
RLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVL
FDIDKFKSINDSFGYTAGDKALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLVWK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=127, Percent_Identity=43.3070866141732, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082007, Length=126, Percent_Identity=37.3015873015873, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI145693134, Length=127, Percent_Identity=35.4330708661417, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786584, Length=126, Percent_Identity=34.1269841269841, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=29.4871794871795, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081881, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=39, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081974, Length=101, Percent_Identity=31.6831683168317, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=29.4478527607362, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 60224; Mature: 60224

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LNQSLEELCFALEQQKDISTLIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAVKIQCANPNLALNELTQQADR
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
ELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEQLKLKMAPLLREMSSLSERLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVLFDIDKFKSINDSFGYTAGDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCHHH
ALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
HHHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCC
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLVWK
EEEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLYMGVLEQDIQAELQKLKSQLEQVRLTQRDTSFKFNREQQVLKRMVTSLATTCRGSSSR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LNQSLEELCFALEQQKDISTLIPKLAVLERMLKQQSLAMDKQTSHLDSQLQHSGETLLRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PGLPAKIKRDLRDLLSFSAAQPLEKTQQALRLLEIYERAVKIQCANPNLALNELTQQADR
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
ELLHRLAVDLQHLITELDFNGESGDQLTDIRTKLLLGVNTHTLLELTLEVLRLVVDGSHA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHH
ERKAAEAFLEQVNLSIGNNLKSATQNLEQSQSYMAHRQEMNGELSSLVARSQTALKEQTE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEQLKLKMAPLLREMSSLSERLLLAEQREQALLERMRYSKDQMEALSDLAQDYRRRLEDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALRAQLDPLTKVYNRSSFTERLEHEYRRWIRTQHNLRVVLFDIDKFKSINDSFGYTAGDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCCHHH
ALSIIARTIKKELRDSDTVARFSGEEFILLLPERSDNESYQIIHQIQLNVSKLPFKFRDK
HHHHHHHHHHHHHCCCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEECCC
SLTITLSAASIRFMDSDTPETVLDRLNLTLSEAKHIGPSQLVWK
EEEEEEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]