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The map label for this gene is ppnK

Identifier: 227081029

GI number: 227081029

Start: 875979

End: 876863

Strand: Reverse

Name: ppnK

Synonym: VCM66_0810

Alternate gene names: 227081029

Gene position: 876863-875979 (Counterclockwise)

Preceding gene: 227081033

Following gene: 227081028

Centisome position: 30.31

GC content: 46.89

Gene sequence:

>885_bases
ATGAAAAAGCCGTTCAACGTTCTTGCCATCATTGGAAAACCGCGCGATCAACAAGCCATTCAAACTCATAAAGAGATTTA
CCACTGGCTAAGATCGCTCGGATATACCGTGTTTATCGATGATCGTTTACGGGAAATTCTGACCGATTTACCCACCGAAC
ATTTTGCAAGCCTGATTGAACTGGGCAAAAAAGCCGATTTGGCGATTGTGGTCGGTGGCGATGGCAACATGCTTGGTGCT
GCGCGCGTGTTGTCCCGCTTTGATATCAGTGTGATCGGGGTTAATCGTGGCAATTTAGGCTTTTTGACCGATTTAAACCC
AGAAGATTTCCAACAGCGCTTACAAGAAGTGTTGGATGGTCACTATTTGCAAGAAACTCGCTTTTTGCTTGAAGCGGAAA
TCCATCGCCACGGTCAAGTGAAAAGCCATAATGCCGCCTTGAATGAAGCCGTGCTTCATCCAGGAAAAATAGCGCATATG
ATTGAATTTGAAGTCTATATTGATGATAACTTCGCTTTCTCTCAGCGCTCCGATGGGTTAATTGTCTCAACCCCAACCGG
CTCGACTGCTTATTCACTTTCCGGCGGAGGCCCGATCCTCTCGCCAAGCCTGAATGCCATTACTTTAGTGCCTATGTTTC
CGCATACCCTCTCCTGCCGCCCATTAGTGGTGGGCGGAAATCAACGCATTAAACTGGTGGTTTCTCCTGAAAATCGCGGC
ACTCAAGAAGTGAGCTGTGATGGACAAGTCTCATTACCTGTGTCGCCTGGTGATGAAATCCACATCTATCAAAGCCCTAA
TGTGCTGAAACTGATCCATCCTCAAGACTACAGTTACTACCACGTATTGCGTACTAAGCTTGGCTGGTCGAGCAAACTGT
TTTGA

Upstream 100 bases:

>100_bases
ATCGTCGATTTCCCAACACAAAAATTCGCATAAAAGCACAACTTGCCCTTATTATGGGGACGATGAATTCTGATTCAAGC
CTAAATCGGTTGGAAACCTT

Downstream 100 bases:

>100_bases
TTTCGTTCACAGATTTTGCTCATAGAGCGTAAAAGTGACCAAAAAATTTTACCTGAGTGACTTTACTGTATAAAGAAACA
GTATAAACTGTTTAAACATA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKKPFNVLAIIGKPRDQQAIQTHKEIYHWLRSLGYTVFIDDRLREILTDLPTEHFASLIELGKKADLAIVVGGDGNMLGA
ARVLSRFDISVIGVNRGNLGFLTDLNPEDFQQRLQEVLDGHYLQETRFLLEAEIHRHGQVKSHNAALNEAVLHPGKIAHM
IEFEVYIDDNFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSPSLNAITLVPMFPHTLSCRPLVVGGNQRIKLVVSPENRG
TQEVSCDGQVSLPVSPGDEIHIYQSPNVLKLIHPQDYSYYHVLRTKLGWSSKLF

Sequences:

>Translated_294_residues
MKKPFNVLAIIGKPRDQQAIQTHKEIYHWLRSLGYTVFIDDRLREILTDLPTEHFASLIELGKKADLAIVVGGDGNMLGA
ARVLSRFDISVIGVNRGNLGFLTDLNPEDFQQRLQEVLDGHYLQETRFLLEAEIHRHGQVKSHNAALNEAVLHPGKIAHM
IEFEVYIDDNFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSPSLNAITLVPMFPHTLSCRPLVVGGNQRIKLVVSPENRG
TQEVSCDGQVSLPVSPGDEIHIYQSPNVLKLIHPQDYSYYHVLRTKLGWSSKLF
>Mature_294_residues
MKKPFNVLAIIGKPRDQQAIQTHKEIYHWLRSLGYTVFIDDRLREILTDLPTEHFASLIELGKKADLAIVVGGDGNMLGA
ARVLSRFDISVIGVNRGNLGFLTDLNPEDFQQRLQEVLDGHYLQETRFLLEAEIHRHGQVKSHNAALNEAVLHPGKIAHM
IEFEVYIDDNFAFSQRSDGLIVSTPTGSTAYSLSGGGPILSPSLNAITLVPMFPHTLSCRPLVVGGNQRIKLVVSPENRG
TQEVSCDGQVSLPVSPGDEIHIYQSPNVLKLIHPQDYSYYHVLRTKLGWSSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=330, Percent_Identity=29.0909090909091, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788968, Length=294, Percent_Identity=58.5034013605442, Blast_Score=355, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI71999610, Length=144, Percent_Identity=34.7222222222222, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320794, Length=317, Percent_Identity=29.6529968454259, Blast_Score=138, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30.8333333333333, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6325068, Length=213, Percent_Identity=30.9859154929577, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573826, Length=254, Percent_Identity=32.6771653543307, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573832, Length=254, Percent_Identity=32.6771653543307, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573828, Length=254, Percent_Identity=32.6771653543307, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573830, Length=254, Percent_Identity=32.6771653543307, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161077047, Length=254, Percent_Identity=32.6771653543307, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653424, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI281363321, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24653422, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281363323, Length=278, Percent_Identity=28.4172661870504, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI20129957, Length=278, Percent_Identity=28.4172661870504, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_VIBC3 (A5F368)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F368
- SMR:   A5F368
- STRING:   A5F368
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0379
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   PRK03378
- BioCyc:   VCHO345073:VC0395_A0379-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32699; Mature: 32699

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFNVLAIIGKPRDQQAIQTHKEIYHWLRSLGYTVFIDDRLREILTDLPTEHFASLIE
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCHHHHHHHHH
LGKKADLAIVVGGDGNMLGAARVLSRFDISVIGVNRGNLGFLTDLNPEDFQQRLQEVLDG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCC
HYLQETRFLLEAEIHRHGQVKSHNAALNEAVLHPGKIAHMIEFEVYIDDNFAFSQRSDGL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCEEECCCCCCE
IVSTPTGSTAYSLSGGGPILSPSLNAITLVPMFPHTLSCRPLVVGGNQRIKLVVSPENRG
EEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCC
TQEVSCDGQVSLPVSPGDEIHIYQSPNVLKLIHPQDYSYYHVLRTKLGWSSKLF
CCEECCCCEEEEECCCCCEEEEEECCCEEEEECCCCCCEEEEEEHHCCCCCCCC
>Mature Secondary Structure
MKKPFNVLAIIGKPRDQQAIQTHKEIYHWLRSLGYTVFIDDRLREILTDLPTEHFASLIE
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCHHHHHHHHH
LGKKADLAIVVGGDGNMLGAARVLSRFDISVIGVNRGNLGFLTDLNPEDFQQRLQEVLDG
CCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHCC
HYLQETRFLLEAEIHRHGQVKSHNAALNEAVLHPGKIAHMIEFEVYIDDNFAFSQRSDGL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEEEEEEECCCEEECCCCCCE
IVSTPTGSTAYSLSGGGPILSPSLNAITLVPMFPHTLSCRPLVVGGNQRIKLVVSPENRG
EEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCCC
TQEVSCDGQVSLPVSPGDEIHIYQSPNVLKLIHPQDYSYYHVLRTKLGWSSKLF
CCEECCCCEEEEECCCCCEEEEEECCCEEEEECCCCCCEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA