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The map label for this gene is tcpJ

Identifier: 227081016

GI number: 227081016

Start: 858178

End: 858939

Strand: Direct

Name: tcpJ

Synonym: VCM66_0796

Alternate gene names: 227081016

Gene position: 858178-858939 (Clockwise)

Preceding gene: 227081015

Following gene: 227081017

Centisome position: 29.67

GC content: 31.5

Gene sequence:

>762_bases
ATGGAATACGTTTACTTGATCCTATTTTCGATTGTATCACTGATTTTGGGTAGTTTTAGTAATGTTGTGATTTATCGTCT
ACCAAGAAAAATTTTACTAAAGAATCATTTTTTTTATGACATCGATTCAAATCGTTCCATGTGCCCTAAATGTGGAAATA
AAATCAGTTGGTATGATAATGTTCCATTGTTAAGTTATTTGTTACTTCATGGTAAGTGTAGACATTGTGATGAAAAAATT
TCATTAAGTTATTTTATTGTCGAATTATCATTTTTTATAATCGCATTTCCAATATATTGGTTATCAACTGACTGGGTTGA
TTCGTTCGTATTGCTCGGGCTTTACTTTATTTTATTCAATCTTTTTGTAATTGATTTTAAATCTATGCTATTGCCAAATT
TACTTACGTATCCGATATTCATGTTGGCATTTATTTATGTGCAGCAAAATCCAGCATTAACAGTTGAAAGCTCTATTATT
GGTGGTTTTGCGGCTTTCATCATATCCTATGTGAGTAATTTCATTGTTCGGTTATTCAAAAGAATCGATGTGATGGGAGG
TGGAGATATAAAGCTCTATACTGCGATTGGTACTCTTATCGGTGTTGAGTTTGTACCATATTTATTCTTGCTGTCCTCGA
TTATTGCATTTATTCATTGGTTCTTTGCTAGAGTCTCATGTCGGTATTGCTTATATATCCCTCTTGGACCAAGCATTATT
ATCTCTTTTGTAATTGTTTTTTTTTCAATCCGTTTAATGTGA

Upstream 100 bases:

>100_bases
GGTTTGCTAGCGTTTCATATTTTTCTACAGTATTTAAGTCAACCATGAATGTAGCACCAAGTGAATATTTATTTATGTTG
ACAGGAGTTGCAGAAAAATA

Downstream 100 bases:

>100_bases
GCTAAAATGAAGTTTTCTATCAAGTTACTAATGATTTTTACATCAATTATAATTACTATTTCATCTATCCTTACTTACTT
TCAGATGCATGGAACCAATG

Product: leader peptidase TcpJ

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSNRSMCPKCGNKISWYDNVPLLSYLLLHGKCRHCDEKI
SLSYFIVELSFFIIAFPIYWLSTDWVDSFVLLGLYFILFNLFVIDFKSMLLPNLLTYPIFMLAFIYVQQNPALTVESSII
GGFAAFIISYVSNFIVRLFKRIDVMGGGDIKLYTAIGTLIGVEFVPYLFLLSSIIAFIHWFFARVSCRYCLYIPLGPSII
ISFVIVFFSIRLM

Sequences:

>Translated_253_residues
MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSNRSMCPKCGNKISWYDNVPLLSYLLLHGKCRHCDEKI
SLSYFIVELSFFIIAFPIYWLSTDWVDSFVLLGLYFILFNLFVIDFKSMLLPNLLTYPIFMLAFIYVQQNPALTVESSII
GGFAAFIISYVSNFIVRLFKRIDVMGGGDIKLYTAIGTLIGVEFVPYLFLLSSIIAFIHWFFARVSCRYCLYIPLGPSII
ISFVIVFFSIRLM
>Mature_253_residues
MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSNRSMCPKCGNKISWYDNVPLLSYLLLHGKCRHCDEKI
SLSYFIVELSFFIIAFPIYWLSTDWVDSFVLLGLYFILFNLFVIDFKSMLLPNLLTYPIFMLAFIYVQQNPALTVESSII
GGFAAFIISYVSNFIVRLFKRIDVMGGGDIKLYTAIGTLIGVEFVPYLFLLSSIIAFIHWFFARVSCRYCLYIPLGPSII
ISFVIVFFSIRLM

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tcpA pilin precursor during membrane translocation

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family

Homologues:

Organism=Escherichia coli, GI87082194, Length=258, Percent_Identity=30.2325581395349, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP4_VIBC3 (A5F385)

Other databases:

- EMBL:   M74708
- EMBL:   L25661
- EMBL:   AF325733
- EMBL:   CP000627
- EMBL:   CP001235
- PIR:   A40582
- STRING:   A5F385
- MEROPS:   A24.001
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0364
- eggNOG:   COG1989
- HOGENOM:   HBG710101
- OMA:   YAFRESE
- ProtClustDB:   CLSK793816
- BioCyc:   VCHO345073:VC0395_A0364-MONOMER
- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045
- PRINTS:   PR00864

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24

EC number: =3.4.23.43

Molecular weight: Translated: 29338; Mature: 29338

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x25502f68)-; HASH(0x25894d44)-; HASH(0x25999d9c)-; HASH(0x24d64408)-; HASH(0x25c9b718)-; HASH(0x25a6e228)-; HASH(0x25c4da4c)-;

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSNRSMCPKCGNKISWYDN
CHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCEEEEECCCCCCCHHCCCCCCCCCC
VPLLSYLLLHGKCRHCDEKISLSYFIVELSFFIIAFPIYWLSTDWVDSFVLLGLYFILFN
CHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFVIDFKSMLLPNLLTYPIFMLAFIYVQQNPALTVESSIIGGFAAFIISYVSNFIVRLFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RIDVMGGGDIKLYTAIGTLIGVEFVPYLFLLSSIIAFIHWFFARVSCRYCLYIPLGPSII
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
ISFVIVFFSIRLM
HHHHHHHHHHHCH
>Mature Secondary Structure
MEYVYLILFSIVSLILGSFSNVVIYRLPRKILLKNHFFYDIDSNRSMCPKCGNKISWYDN
CHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCEEEEECCCCCCCHHCCCCCCCCCC
VPLLSYLLLHGKCRHCDEKISLSYFIVELSFFIIAFPIYWLSTDWVDSFVLLGLYFILFN
CHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFVIDFKSMLLPNLLTYPIFMLAFIYVQQNPALTVESSIIGGFAAFIISYVSNFIVRLFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
RIDVMGGGDIKLYTAIGTLIGVEFVPYLFLLSSIIAFIHWFFARVSCRYCLYIPLGPSII
HHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
ISFVIVFFSIRLM
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1680773; 7894059; 11179381