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The map label for this gene is ysgA [H]

Identifier: 227080982

GI number: 227080982

Start: 816719

End: 817492

Strand: Direct

Name: ysgA [H]

Synonym: VCM66_0761

Alternate gene names: 227080982

Gene position: 816719-817492 (Clockwise)

Preceding gene: 227080981

Following gene: 227080984

Centisome position: 28.24

GC content: 52.33

Gene sequence:

>774_bases
TTGCACTATAATTCGCCGGCATTTATCTCGGACAGCACCATGATTTCGAAAACCCAACTCAAATTGATTCGCGCTTTAGG
GCAGAAGAAACAGCGCAAAGCGCACGGCTTATTTCTGGTTCAGGGCGAGAAAAACGTCCTAGAGCTGACCAACAGCACGC
TGAAAGTACAACACATTTTTGCGACCGCCGAGTTTCTCGCCGAGCACGCACAAGCCTTAGCCGGTTTCGAGTGTATTGAA
GCAACCTTGGATGATTTAACCAAAGTCAGCACCTTGGTGAGCAACAACGCCGCGATTGCGGTGGTGGCGATCCCACACAT
CGAGGTGCCTAAAGCACAAGGGTTGATGATTGCGTTGGATGGCGTTTCCGACCCCGGTAACTTGGGCACCATTATTCGTG
TCGCGGATTGGTATGGCATTTCGCACATCATCGCCAGCCAAGATTGCGCCGACCCGTATAACCCGAAAACCATCAGCGCC
ACGATGGGCAGCTTTGGCCGCGTGCAAGTTAGCCAAGTGGATTTACCCGACTACTTAGCCAAGGCGAATTTGCCAGTGTA
CGGTGCATTTTTGGAAGGGGTGAGCGTGCATCAAACCGCTTTTCAAGCCGAAGGCATTTTGCTGATGGGCAGTGAATCTC
ACGGTATTCGCGAGCAAGCGGCGCAGTACGTGACGGATAAGATCACCATTCCGGCCTTTGGCGGCGCAGAGTCACTCAAT
GTCGCGATGGCAACGGGGATCATTCTCGACAATATTCGCCGTCAGCATAGTTAA

Upstream 100 bases:

>100_bases
CATTAGGCGAGTTTGCTCAAGCCTAGCGCTTGGCAATCGAAAGCTGCCATTGTGTTGGTTTGATGGCTTCCCCTACGCGC
CCCTTTAGCTCGTAGGGGCT

Downstream 100 bases:

>100_bases
TCCATTTACCTAAAACGAATTTACCTAAAACGAACATACCTAAAACCAAAAAGCGTCGGACTGTCCGACGCTTTTTTCAT
CTCTAGGCTTTTTTCTTAAG

Product: RNA methyltransferase, TrmH family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MHYNSPAFISDSTMISKTQLKLIRALGQKKQRKAHGLFLVQGEKNVLELTNSTLKVQHIFATAEFLAEHAQALAGFECIE
ATLDDLTKVSTLVSNNAAIAVVAIPHIEVPKAQGLMIALDGVSDPGNLGTIIRVADWYGISHIIASQDCADPYNPKTISA
TMGSFGRVQVSQVDLPDYLAKANLPVYGAFLEGVSVHQTAFQAEGILLMGSESHGIREQAAQYVTDKITIPAFGGAESLN
VAMATGIILDNIRRQHS

Sequences:

>Translated_257_residues
MHYNSPAFISDSTMISKTQLKLIRALGQKKQRKAHGLFLVQGEKNVLELTNSTLKVQHIFATAEFLAEHAQALAGFECIE
ATLDDLTKVSTLVSNNAAIAVVAIPHIEVPKAQGLMIALDGVSDPGNLGTIIRVADWYGISHIIASQDCADPYNPKTISA
TMGSFGRVQVSQVDLPDYLAKANLPVYGAFLEGVSVHQTAFQAEGILLMGSESHGIREQAAQYVTDKITIPAFGGAESLN
VAMATGIILDNIRRQHS
>Mature_257_residues
MHYNSPAFISDSTMISKTQLKLIRALGQKKQRKAHGLFLVQGEKNVLELTNSTLKVQHIFATAEFLAEHAQALAGFECIE
ATLDDLTKVSTLVSNNAAIAVVAIPHIEVPKAQGLMIALDGVSDPGNLGTIIRVADWYGISHIIASQDCADPYNPKTISA
TMGSFGRVQVSQVDLPDYLAKANLPVYGAFLEGVSVHQTAFQAEGILLMGSESHGIREQAAQYVTDKITIPAFGGAESLN
VAMATGIILDNIRRQHS

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=289, Percent_Identity=24.5674740484429, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1790623, Length=159, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666840, Length=281, Percent_Identity=24.5551601423488, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27596; Mature: 27596

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYNSPAFISDSTMISKTQLKLIRALGQKKQRKAHGLFLVQGEKNVLELTNSTLKVQHIF
CCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCEEEEEHHH
ATAEFLAEHAQALAGFECIEATLDDLTKVSTLVSNNAAIAVVAIPHIEVPKAQGLMIALD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEECCCCCCEEEEEE
GVSDPGNLGTIIRVADWYGISHIIASQDCADPYNPKTISATMGSFGRVQVSQVDLPDYLA
CCCCCCCCCEEEEEECHHCHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCHHHHH
KANLPVYGAFLEGVSVHQTAFQAEGILLMGSESHGIREQAAQYVTDKITIPAFGGAESLN
HCCCCEEEHHHCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCEEECCCCCCCCCH
VAMATGIILDNIRRQHS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHYNSPAFISDSTMISKTQLKLIRALGQKKQRKAHGLFLVQGEKNVLELTNSTLKVQHIF
CCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCEEEEEHHH
ATAEFLAEHAQALAGFECIEATLDDLTKVSTLVSNNAAIAVVAIPHIEVPKAQGLMIALD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEECCCCCCEEEEEE
GVSDPGNLGTIIRVADWYGISHIIASQDCADPYNPKTISATMGSFGRVQVSQVDLPDYLA
CCCCCCCCCEEEEEECHHCHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEECCHHHHH
KANLPVYGAFLEGVSVHQTAFQAEGILLMGSESHGIREQAAQYVTDKITIPAFGGAESLN
HCCCCEEEHHHCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCEEECCCCCCCCCH
VAMATGIILDNIRRQHS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]