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The map label for this gene is trmJ [H]

Identifier: 227080925

GI number: 227080925

Start: 757254

End: 757988

Strand: Direct

Name: trmJ [H]

Synonym: VCM66_0704

Alternate gene names: 227080925

Gene position: 757254-757988 (Clockwise)

Preceding gene: 227080923

Following gene: 227080926

Centisome position: 26.18

GC content: 51.16

Gene sequence:

>735_bases
ATGATGTTAGAACGCGTGAAAGTCGTATTGGTCGGCACAACTCACTCTGGAAATATTGGTTCGGCCGCTCGGGCGATGAA
AGTCATGGGGCTGAGCCAGATGGTGCTGGTCGATCCGCAGTGTCAGGTTGATGCTCAAGCGATCGCGTTAGCCGCGGGGG
CAAGTGAAATTGCACTCAATGCGCAAATTTATCCAACGCTGGAAGCCGCCGTTGCTGATTGTGGGTTAGTCGTGGGCACC
AGTGCGCGTTCACGCACCCTTGAATGGCCAATGCTTGAGCCACGCGAGTGCGGAGAAAAACTCATCAGCGAAGCCAAGCA
ACATAGTGTGGCTATGGTGTTTGGTCGTGAACGTACTGGCTTGACCAATGATGAGCTGCAACTGTGTCACTACCACGTGT
GTGTGCCTGCGAATCCAGAATACAGCTCACTGAATTTAGCAATGGCGGTTCAACTGCTGAGTTATGAAGTGCGGATGGCC
TATCTTGCTTTGCAGCAGAGCTCGCAAAGCAGCACATTGCAGGAAGAGTATCCGCGTCATCAAGAGTTGGAGCGTTTTTA
TGCCCATCTTGAACAAGTGATAATGCAAACCGAGTTTATTTCTGCGCAGCAGCCGGGGCAGGTGATGAATAAACTGCGCC
GCATGTTTACGCGCGCTCGGCCAGAAGCTCAAGAAATCAATATCTTGCGCGGGATCTTAACGTCAGTACAAAAGTCAATC
TCACGCAAAGAATAA

Upstream 100 bases:

>100_bases
TTTTTTGTGCGCTTAGTTGCCCGTCAAGGTAAAGGGTCTGACCATTTTGGTCAGGTTTGTGTTAATATCCCGCGGCTTTG
TTTGGAGTACATTTTAGCAA

Downstream 100 bases:

>100_bases
GCTTATAAGTCTCAGGGTTAAATACCTGACTAAATTAGTCAAATAAATACTTGACCAAAAGAGTCAAGTATGGAAAACTT
CCAGCCACATAAACAGTGTG

Product: RNA methyltransferase, TrmH family

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMLERVKVVLVGTTHSGNIGSAARAMKVMGLSQMVLVDPQCQVDAQAIALAAGASEIALNAQIYPTLEAAVADCGLVVGT
SARSRTLEWPMLEPRECGEKLISEAKQHSVAMVFGRERTGLTNDELQLCHYHVCVPANPEYSSLNLAMAVQLLSYEVRMA
YLALQQSSQSSTLQEEYPRHQELERFYAHLEQVIMQTEFISAQQPGQVMNKLRRMFTRARPEAQEINILRGILTSVQKSI
SRKE

Sequences:

>Translated_244_residues
MMLERVKVVLVGTTHSGNIGSAARAMKVMGLSQMVLVDPQCQVDAQAIALAAGASEIALNAQIYPTLEAAVADCGLVVGT
SARSRTLEWPMLEPRECGEKLISEAKQHSVAMVFGRERTGLTNDELQLCHYHVCVPANPEYSSLNLAMAVQLLSYEVRMA
YLALQQSSQSSTLQEEYPRHQELERFYAHLEQVIMQTEFISAQQPGQVMNKLRRMFTRARPEAQEINILRGILTSVQKSI
SRKE
>Mature_244_residues
MMLERVKVVLVGTTHSGNIGSAARAMKVMGLSQMVLVDPQCQVDAQAIALAAGASEIALNAQIYPTLEAAVADCGLVVGT
SARSRTLEWPMLEPRECGEKLISEAKQHSVAMVFGRERTGLTNDELQLCHYHVCVPANPEYSSLNLAMAVQLLSYEVRMA
YLALQQSSQSSTLQEEYPRHQELERFYAHLEQVIMQTEFISAQQPGQVMNKLRRMFTRARPEAQEINILRGILTSVQKSI
SRKE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=63.4453781512605, Blast_Score=320, Evalue=4e-89,
Organism=Escherichia coli, GI1790865, Length=174, Percent_Identity=36.7816091954023, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27121; Mature: 27121

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLERVKVVLVGTTHSGNIGSAARAMKVMGLSQMVLVDPQCQVDAQAIALAAGASEIALN
CCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCHHEEEC
AQIYPTLEAAVADCGLVVGTSARSRTLEWPMLEPRECGEKLISEAKQHSVAMVFGRERTG
CEECHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCC
LTNDELQLCHYHVCVPANPEYSSLNLAMAVQLLSYEVRMAYLALQQSSQSSTLQEEYPRH
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCH
QELERFYAHLEQVIMQTEFISAQQPGQVMNKLRRMFTRARPEAQEINILRGILTSVQKSI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SRKE
HCCC
>Mature Secondary Structure
MMLERVKVVLVGTTHSGNIGSAARAMKVMGLSQMVLVDPQCQVDAQAIALAAGASEIALN
CCCCEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCHHEEEC
AQIYPTLEAAVADCGLVVGTSARSRTLEWPMLEPRECGEKLISEAKQHSVAMVFGRERTG
CEECHHHHHHHHHCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCC
LTNDELQLCHYHVCVPANPEYSSLNLAMAVQLLSYEVRMAYLALQQSSQSSTLQEEYPRH
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCH
QELERFYAHLEQVIMQTEFISAQQPGQVMNKLRRMFTRARPEAQEINILRGILTSVQKSI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SRKE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA