Click here to switch to the map view.

The map label for this gene is aceB [H]

Identifier: 227080913

GI number: 227080913

Start: 746399

End: 748069

Strand: Direct

Name: aceB [H]

Synonym: VCM66_0692

Alternate gene names: 227080913

Gene position: 746399-748069 (Clockwise)

Preceding gene: 227080911

Following gene: 227080915

Centisome position: 25.8

GC content: 49.67

Gene sequence:

>1671_bases
ATGTTCTTGGTGAGTGTTTCAAGGAAGGAATTTATGTCAATCAATACTGCTGAATTAACCCAACACACCACGGAAACCAA
AATCACCCAAGGCAAGCTTGCTGTTGTGGGTACCCTTTCACCCGAACATCAAGCGATTTTCCCTGTCGAAGCCCAAACTT
TTTTACAGCAACTCTGTGAGCGCTTTGCGCCGCGAGTGGATGAATTACTGACCTTGCGTGAACAAAAACAAACGCTTATC
GACCAAGGCCAGCTACCTGATTTTCTTCCTGAAACCAAAGATATCCGTGAAGGAAGCTGGAAGATTCTCGGCATTCCAGA
CGATCTGCAAGATCGTCGCGTAGAGATCACGGGGCCAACCGATCGCAAAATGGTGATTAATGCACTGAACGCGAATGTGA
AAGTCTTTATGGCTGACTTTGAAGATTCGATGTCGCCCGCGTGGGATAAGGTGTTGGATGGGCAGATCAACCTGCGTGAT
GCGATTCTGGGCAGCATAAGCTATACCAACCCAGACAATGGCAAGCGTTATGAGCTCAATGCCAACCCAGCGGTGCTCAT
CTGCCGCGTGCGCGGTCTGCATCTCAAAGAAAAACATGTCACCTACAACGGCTTGGCGATTCCCGGTTCACTGTTCGATT
TTGCGCTCTATTTTTACAACAACCATCGCGCACTATTGAAAAAAGGCAGTGGCCCTTATTTCTACCTACCTAAGCTGCAA
GCTTATCAAGAGGCGCAGTGGTGGAGTGACGTGTTCCATTTCACGGAAGATTATTTTGGCTTAGACACAGGCACCATCAA
AGCAACGGTGTTGATTGAAACTCTGCCTGCGGTATTTCAGATGGATGAGATCCTCTTCTCACTCAAAGAGCACATTGTCG
GTCTGAACTGTGGACGCTGGGATTATATTTTCAGCTACATCAAAACCTTGAAAAAGCATCCCGACCGCGTATTGCCGGAT
CGCCAAGTGGTGACCATGGATAAGCCTTTCCTCAATGCGTATTCCCGTTTGTTGATTTACACCTGTCATAAACGCGGCGC
GTTTGCGATGGGCGGGATGGCGGCGTTTATTCCAGCCAAAGATCCTGCGCTCAATGAACAAGTGCTACAAAAAATTCGCG
GCGACAAAATGCTTGAAGCGACCAATGGCCACGATGGCACTTGGGTTGCTCACCCCGGTTTAGCGGATACCGCGATGGCG
GTGTTTAACGAAGTGCTTGGTGAGCGCAAAAACCAACTCAATGTGAGCCGGATGGCCGATGCTCCGATAACCGCGGCCGA
GCTATTGGCACCTTGCGATGGATCACGCTCTGAGCATGGCATGCGCCACAACATTCGCGTTGCGTTGCAATACATCGAAG
CATGGATTTCTGGTAACGGTTGTGTGCCGATCTACGGCTTGATGGAAGATGCGGCGACAGCGGAGATTTCACGCGCTTCG
ATTTGGCAATGGATCCAACACGGCAAAACGTTAGATAACGGGCAAGTGGTCACTAACGAACTGTTTCGCGACTACCTCAA
ACAAGAGATTGAGGTGGTGAAAAGCGAAGTGGGTGAGTCCCGTTTTGCACAAGGGCGTTTTACTGAAGCGGCCGAATTGA
TGGAGCGCTTAACGACCAGCCAAGAGCTACCGAATTTCTTAACCATACCTGGCTACGACTACTTGCCGTAG

Upstream 100 bases:

>100_bases
CTAGTGCCCGGAGTCTTGCAAAAGTGCGCAATTTAAGTGACTTTAAATCACAACGGATAAGCCAACTGAATAATGAAAAT
TTGTAGTTTTAAAGTTACAA

Downstream 100 bases:

>100_bases
GCCTCTATAACACCAGTAAGGTGAAAAAACACCAGTAACGTGAAACCACTTTACGATCAGCGAGCGCAGTACTGATCGAA
CAAGGAATCGAGCTGCCAAC

Product: malate synthase

Products: NA

Alternate protein names: MSA [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLTLREQKQTLI
DQGQLPDFLPETKDIREGSWKILGIPDDLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGSISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPGSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAAELLAPCDGSRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP

Sequences:

>Translated_556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLTLREQKQTLI
DQGQLPDFLPETKDIREGSWKILGIPDDLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGSISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPGSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAAELLAPCDGSRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP
>Mature_556_residues
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCERFAPRVDELLTLREQKQTLI
DQGQLPDFLPETKDIREGSWKILGIPDDLQDRRVEITGPTDRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRD
AILGSISYTNPDNGKRYELNANPAVLICRVRGLHLKEKHVTYNGLAIPGSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRWDYIFSYIKTLKKHPDRVLPD
RQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAKDPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMA
VFNEVLGERKNQLNVSRMADAPITAAELLAPCDGSRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTSQELPNFLTIPGYDYLP

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=532, Percent_Identity=71.2406015037594, Blast_Score=795, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17561814, Length=515, Percent_Identity=51.0679611650485, Blast_Score=505, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71982926, Length=409, Percent_Identity=53.5452322738386, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324212, Length=519, Percent_Identity=46.8208092485549, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322222, Length=519, Percent_Identity=45.4720616570328, Blast_Score=437, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 62859; Mature: 62859

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCE
CEEEEECHHHHHCCCHHHHHHHHHHHEEECCCEEEEEECCCCCCEEECCHHHHHHHHHHH
RFAPRVDELLTLREQKQTLIDQGQLPDFLPETKDIREGSWKILGIPDDLQDRRVEITGPT
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCC
DRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRDAILGSISYTNPDNGKRYELN
CCHHHHEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCEEEEC
ANPAVLICRVRGLHLKEKHVTYNGLAIPGSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
CCCEEEEEEECCCEEECCEEEECCEECCHHHHHEEHHHCCCCCEEEECCCCCEEECCCHH
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRW
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DYIFSYIKTLKKHPDRVLPDRQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAK
HHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCEEECCEEEECCCC
DPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMAVFNEVLGERKNQLNVSRMAD
CCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
APITAAELLAPCDGSRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
QELPNFLTIPGYDYLP
HHCCCEEECCCCCCCC
>Mature Secondary Structure
MFLVSVSRKEFMSINTAELTQHTTETKITQGKLAVVGTLSPEHQAIFPVEAQTFLQQLCE
CEEEEECHHHHHCCCHHHHHHHHHHHEEECCCEEEEEECCCCCCEEECCHHHHHHHHHHH
RFAPRVDELLTLREQKQTLIDQGQLPDFLPETKDIREGSWKILGIPDDLQDRRVEITGPT
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCC
DRKMVINALNANVKVFMADFEDSMSPAWDKVLDGQINLRDAILGSISYTNPDNGKRYELN
CCHHHHEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCEEEEC
ANPAVLICRVRGLHLKEKHVTYNGLAIPGSLFDFALYFYNNHRALLKKGSGPYFYLPKLQ
CCCEEEEEEECCCEEECCEEEECCEECCHHHHHEEHHHCCCCCEEEECCCCCEEECCCHH
AYQEAQWWSDVFHFTEDYFGLDTGTIKATVLIETLPAVFQMDEILFSLKEHIVGLNCGRW
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DYIFSYIKTLKKHPDRVLPDRQVVTMDKPFLNAYSRLLIYTCHKRGAFAMGGMAAFIPAK
HHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHEEEECCCCCEEECCEEEECCCC
DPALNEQVLQKIRGDKMLEATNGHDGTWVAHPGLADTAMAVFNEVLGERKNQLNVSRMAD
CCCCCHHHHHHHCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHCC
APITAAELLAPCDGSRSEHGMRHNIRVALQYIEAWISGNGCVPIYGLMEDAATAEISRAS
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
IWQWIQHGKTLDNGQVVTNELFRDYLKQEIEVVKSEVGESRFAQGRFTEAAELMERLTTS
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHH
QELPNFLTIPGYDYLP
HHCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3050899; 3060852; 8265357; 9278503 [H]