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The map label for this gene is aroF [H]

Identifier: 227080876

GI number: 227080876

Start: 700982

End: 702055

Strand: Direct

Name: aroF [H]

Synonym: VCM66_0653

Alternate gene names: 227080876

Gene position: 700982-702055 (Clockwise)

Preceding gene: 227080873

Following gene: 227080877

Centisome position: 24.23

GC content: 48.79

Gene sequence:

>1074_bases
ATGAAAAAAAGTGAATTGAGTGATGTGAATATCAGTAATGAACAGATATTGATCACCCCAGATGCCTTAAAAGCCAAGAT
TCCACTCAGTGACAAGGCGCGTAAGTTTATTCGTGAGTCACGTCAGACGGTGGCGGACATTATCCACAAACGCGATCCTC
GCTTGCTGATCGTCTGTGGTCCTTGCTCAATTCATGATGTGGATGCGGCGAAAGAGTACGCCAAAAAACTCAAAGCACTC
TCGGCGCAGCTGAGCGACCAGCTCTACATCGTAATGCGCGTGTACTTTGAAAAACCGCGTACTACGGTCGGTTGGAAAGG
GCTGATTAACGATCCGCATCTTGATGGCAGTTTTGATATCGAACACGGTCTGCATGTTGGGCGTCAACTCTTGGTCGATT
TGGCCGAAATGGAAATTCCACTGGCAACCGAAGCGTTGGACCCGATTAGCCCGCAATACTTGGCGGATACTTTTAGTTGG
GCGGCGATTGGTGCGCGTACCACGGAATCACAAACTCACCGCGAGATGGCAAGTGGCCTTTCCATGCCAATCGGCTTTAA
AAATGGCACGGATGGTAACTTGGCAACGGCGATTAATGCCATGCAGGCCGCTTCATCCAGTCACCGCTTTATGGGAATTA
ACCGCGAAGGACAAGTGGCACTGCTCACGACACAAGGCAACCCGAATGGGCATGTGATTTTACGTGGTGGCAAACAGACC
AACTACGATTCGGTTTCTGTCGCGGAATGTGAAGAAGAGATGCAAAAAGCGCGTTTGGAACCCTCTTTGATGGTGGATTG
CAGCCATGCGAACTCACGTAAAGATTACCGCCGTCAGCCGTTGGTGGCAGAAGATGTGATTCATCAAATCCGTGAAGGCA
ATCGCTCGATCATTGGCCTGATGATTGAAAGCCATCTCAATGAAGGAAATCAGTCTTCGGATCTGCCTCGTGAAAAGATG
CAATACGGAGTCTCGATCACTGACGGCTGTATAAATTGGTCAACGACTGAGGCATTATTACGTCGTGCGCATCAGGAACT
TATTCCCTTTTTGCACAATCGTTTGCAAGGATAA

Upstream 100 bases:

>100_bases
ATTTACACTTGCATTGTTTTTAAAGCCTGTTAACGTGTTTTCTGTTTAAGTTTGCAGCTTACCTTGTTAGGTGTGTAAAT
AATAAATTACAGGTGATGAG

Downstream 100 bases:

>100_bases
CAAGGATAGAACATGGCCGTAGAGTTGAATCAGTTACGCGACCAAATCGATGAAGTCGATAAGCAGATGGTGGAGCTACT
GGCGCGCCGTCTGGCATTGG

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKSELSDVNISNEQILITPDALKAKIPLSDKARKFIRESRQTVADIIHKRDPRLLIVCGPCSIHDVDAAKEYAKKLKAL
SAQLSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVDLAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGNLATAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEEEMQKARLEPSLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNRSIIGLMIESHLNEGNQSSDLPREKM
QYGVSITDGCINWSTTEALLRRAHQELIPFLHNRLQG

Sequences:

>Translated_357_residues
MKKSELSDVNISNEQILITPDALKAKIPLSDKARKFIRESRQTVADIIHKRDPRLLIVCGPCSIHDVDAAKEYAKKLKAL
SAQLSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVDLAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGNLATAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEEEMQKARLEPSLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNRSIIGLMIESHLNEGNQSSDLPREKM
QYGVSITDGCINWSTTEALLRRAHQELIPFLHNRLQG
>Mature_357_residues
MKKSELSDVNISNEQILITPDALKAKIPLSDKARKFIRESRQTVADIIHKRDPRLLIVCGPCSIHDVDAAKEYAKKLKAL
SAQLSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDIEHGLHVGRQLLVDLAEMEIPLATEALDPISPQYLADTFSW
AAIGARTTESQTHREMASGLSMPIGFKNGTDGNLATAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
NYDSVSVAECEEEMQKARLEPSLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNRSIIGLMIESHLNEGNQSSDLPREKM
QYGVSITDGCINWSTTEALLRRAHQELIPFLHNRLQG

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=347, Percent_Identity=69.4524495677233, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786969, Length=335, Percent_Identity=54.0298507462687, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787996, Length=326, Percent_Identity=48.4662576687117, Blast_Score=333, Evalue=9e-93,
Organism=Saccharomyces cerevisiae, GI6319726, Length=339, Percent_Identity=52.8023598820059, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320240, Length=347, Percent_Identity=49.8559077809798, Blast_Score=350, Evalue=2e-97,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 39727; Mature: 39727

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSELSDVNISNEQILITPDALKAKIPLSDKARKFIRESRQTVADIIHKRDPRLLIVCG
CCCCCCCCCCCCCCEEEEECHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PCSIHDVDAAKEYAKKLKALSAQLSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCH
EHGLHVGRQLLVDLAEMEIPLATEALDPISPQYLADTFSWAAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SMPIGFKNGTDGNLATAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYDSVSVAECEEEMQKARLEPSLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNRSIIGL
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEH
MIESHLNEGNQSSDLPREKMQYGVSITDGCINWSTTEALLRRAHQELIPFLHNRLQG
HHHHHCCCCCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKSELSDVNISNEQILITPDALKAKIPLSDKARKFIRESRQTVADIIHKRDPRLLIVCG
CCCCCCCCCCCCCCEEEEECHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PCSIHDVDAAKEYAKKLKALSAQLSDQLYIVMRVYFEKPRTTVGWKGLINDPHLDGSFDI
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCH
EHGLHVGRQLLVDLAEMEIPLATEALDPISPQYLADTFSWAAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SMPIGFKNGTDGNLATAINAMQAASSSHRFMGINREGQVALLTTQGNPNGHVILRGGKQT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYDSVSVAECEEEMQKARLEPSLMVDCSHANSRKDYRRQPLVAEDVIHQIREGNRSIIGL
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCEEEEH
MIESHLNEGNQSSDLPREKMQYGVSITDGCINWSTTEALLRRAHQELIPFLHNRLQG
HHHHHCCCCCCCCCCCHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]