Click here to switch to the map view.

The map label for this gene is hlyU

Identifier: 227080859

GI number: 227080859

Start: 682592

End: 682918

Strand: Direct

Name: hlyU

Synonym: VCM66_0636

Alternate gene names: 227080859

Gene position: 682592-682918 (Clockwise)

Preceding gene: 227080858

Following gene: 227080861

Centisome position: 23.6

GC content: 44.34

Gene sequence:

>327_bases
ATGCCGTATTTAAAGGGGGCACCAATGAATCTCCAAGAGATGGAAAAAAATTCTGCCAAAGCGGTAGTTTTGCTCAAAGC
GATGGCCAATGAGCGGCGACTGCAAATTTTATGTATGTTACTGGACAATGAACTGTCGGTAGGGGAATTGAGTAGCCGTT
TGGAACTCAGCCAATCTGCTCTTTCTCAACATCTAGCGTGGCTACGTCGTGATGGTTTAGTGAATACACGTAAAGAAGCG
CAAACTGTGTTTTATACGTTGAGCAGTACAGAAGTGAAGGCGATGATTGAACTGTTGCACCGTCTTTATTGTCAGGCGAA
TCAGTAG

Upstream 100 bases:

>100_bases
CTCCGCTTTCACCATTTCGACCCATCATGATCACTCAACCGAATTACGACATCGGTGATTAGAACGACAGAGAAAAGTGA
ATGAATGGGTTGGAATTAAA

Downstream 100 bases:

>100_bases
GGTGGTCGAAAAAAAGGTATAAAAAAACCGGCGTAGGCCGGTTTTTTGTTGCTGAAGATCAAACTCTATTACAGAGCGTT
GATAGCAGCAAAGAAGCGAG

Product: transcriptional activator HlyU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MPYLKGAPMNLQEMEKNSAKAVVLLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEA
QTVFYTLSSTEVKAMIELLHRLYCQANQ

Sequences:

>Translated_108_residues
MPYLKGAPMNLQEMEKNSAKAVVLLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEA
QTVFYTLSSTEVKAMIELLHRLYCQANQ
>Mature_107_residues
PYLKGAPMNLQEMEKNSAKAVVLLKAMANERRLQILCMLLDNELSVGELSSRLELSQSALSQHLAWLRRDGLVNTRKEAQ
TVFYTLSSTEVKAMIELLHRLYCQANQ

Specific function: Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789021, Length=95, Percent_Identity=41.0526315789474, Blast_Score=76, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): HLYU_VIBCH (P52695)

Other databases:

- EMBL:   X66866
- EMBL:   AJ002395
- EMBL:   AE003852
- PIR:   S37313
- RefSeq:   NP_230327.1
- ProteinModelPortal:   P52695
- SMR:   P52695
- GeneID:   2615467
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0678
- TIGR:   VC_0678
- HOGENOM:   HBG742701
- OMA:   AMANPKR
- ProtClustDB:   CLSK874069
- GO:   GO:0005622
- GO:   GO:0009405
- InterPro:   IPR001845
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00778
- SMART:   SM00418

Pfam domain/function: PF01022 HTH_5

EC number: NA

Molecular weight: Translated: 12294; Mature: 12162

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00846 HTH_ARSR_1; PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYLKGAPMNLQEMEKNSAKAVVLLKAMANERRLQILCMLLDNELSVGELSSRLELSQSA
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LSQHLAWLRRDGLVNTRKEAQTVFYTLSSTEVKAMIELLHRLYCQANQ
HHHHHHHHHHCCCCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PYLKGAPMNLQEMEKNSAKAVVLLKAMANERRLQILCMLLDNELSVGELSSRLELSQSA
CCCCCCCCCHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LSQHLAWLRRDGLVNTRKEAQTVFYTLSSTEVKAMIELLHRLYCQANQ
HHHHHHHHHHCCCCCCHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8231807; 9457888; 10952301