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The map label for this gene is thyA [H]

Identifier: 227080856

GI number: 227080856

Start: 679016

End: 679867

Strand: Direct

Name: thyA [H]

Synonym: VCM66_0633

Alternate gene names: 227080856

Gene position: 679016-679867 (Clockwise)

Preceding gene: 227080855

Following gene: 227080858

Centisome position: 23.47

GC content: 46.13

Gene sequence:

>852_bases
GTGAGACAGTATTTAGATCTTTGTCAGCGCATCGTCGATCAAGGTGTTTGGGTTGAAAATGAACGAACGGGCAAGCGTTG
TTTGACTGTGATTAATGCCGATTTGACCTACGATGTGGGCAACAATCAGTTTCCTCTAGTCACTACACGCAAGAGTTTTT
GGAAAGCCGCCGTGGCCGAGTTGCTCGGCTATATTCGTGGTTACGATAATGCGGCGGATTTTCGCCAATTAGGTACCAAA
ACCTGGGATGCTAATGCCAATTTAAACCAAGCATGGCTCAACAATCCTTACCGTAAAGGTGAGGATGACATGGGACGCGT
GTATGGAGTTCAGGGTAGAGCTTGGGCTAAGCCTGATGGTGGTCATATTGACCAGTTGAAAAAGATTGTTGATGATTTGA
GCCGTGGCGTTGATGACCGAGGTGAAATTCTTAACTTCTACAATCCGGGTGAATTTCACATGGGGTGTTTGCGCCCTTGC
ATGTACAGCCATCATTTTTCATTGCTGGGTGATACCTTGTATCTCAACAGTACTCAGCGTTCATGTGATGTGCCCTTGGG
GTTGAATTTCAACATGGTGCAGGTTTATGTGTTCCTTGCGCTGATGGCACAGATCACAGGGAAAAAGCCGGGCTTGGCGT
ATCACAAGATCGTCAATGCGCACATTTACCAAGATCAACTCGAATTGATGCGCGATGTGCAGCTAAAACGTGAGCCATTC
CCAGCGCCTCAGTTCCATATCAATCCAAAGATTAAAACACTGCAGGATTTGGAAACTTGGGTCACTTTGGATGATTTTGA
CGTCACCGGATATCAGTTCCACGATCCTATTCAATACCCGTTTTCAGTCTAA

Upstream 100 bases:

>100_bases
CCTCTCCTTACCTATGGTGATCATCGGTATTTTGATGATGGTTTGGTCTTACAAGCGCGGTTTGTATCAAGACCGTGTAG
CAGCAAAATAGGGTAGTTAG

Downstream 100 bases:

>100_bases
TCCCGTATTCAGGCGGTATGGCTTGATGGGTTTTACATAGGAAAAGCTCCCGAAGGTCGGGAGCTTTTTTTATACAGATG
ATGCTTTAACGCTTAAGCGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV

Sequences:

>Translated_283_residues
MRQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
>Mature_283_residues
MRQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAELLGYIRGYDNAADFRQLGTK
TWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDGGHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPC
MYSHHFSLLGDTLYLNSTQRSCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=33.2179930795848, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=32.1554770318021, Blast_Score=147, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI71993377, Length=275, Percent_Identity=27.2727272727273, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324648, Length=302, Percent_Identity=26.8211920529801, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137556, Length=293, Percent_Identity=29.0102389078498, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 32573; Mature: 32573

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRQLGTKTWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
CCCCEEECCCHHHHHHHHHHEEEECCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MRQYLDLCQRIVDQGVWVENERTGKRCLTVINADLTYDVGNNQFPLVTTRKSFWKAAVAE
CCHHHHHHHHHHHCCCEECCCCCCCCEEEEEECCEEEECCCCCCCEEECHHHHHHHHHHH
LLGYIRGYDNAADFRQLGTKTWDANANLNQAWLNNPYRKGEDDMGRVYGVQGRAWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCHHHEEECCCCEECCCCC
GHIDQLKKIVDDLSRGVDDRGEILNFYNPGEFHMGCLRPCMYSHHFSLLGDTLYLNSTQR
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
SCDVPLGLNFNMVQVYVFLALMAQITGKKPGLAYHKIVNAHIYQDQLELMRDVQLKREPF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAPQFHINPKIKTLQDLETWVTLDDFDVTGYQFHDPIQYPFSV
CCCCEEECCCHHHHHHHHHHEEEECCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA