| Definition | Vibrio cholerae M66-2 chromosome I, complete genome. |
|---|---|
| Accession | NC_012578 |
| Length | 2,892,523 |
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The map label for this gene is ptsP [H]
Identifier: 227080853
GI number: 227080853
Start: 675112
End: 677358
Strand: Direct
Name: ptsP [H]
Synonym: VCM66_0630
Alternate gene names: 227080853
Gene position: 675112-677358 (Clockwise)
Preceding gene: 227080852
Following gene: 227080854
Centisome position: 23.34
GC content: 48.82
Gene sequence:
>2247_bases ATGCTTTCTCAGCTAAGGGACATAGTAGAACAAGTTTCAAAAGTCGAAGACGTCTATCAGGCGTTGGATATTTTTGTAAA GCAGACCTGTGAGGCGATGAGCACAGAGTGTTGTACTGTCTATTTAGCGAATGAAGAGATGCACCGTCTGGAATTGATGG CCACACAAGGTCTGAAGTTCAAGGGCGATAAGATCCATATTGATTTTGATGAAGGCTTGGTCGGGTTGGTTAAACGTTCT GCCGAACCGATTAACTTAGCCGAAGCCTCAAAACATCCGAGCTTTAAATATTTTAAGCAGTTGGGTGAAGAGGTTTATCA CTCCTTCCTCGGTACACCGATCATCTATCGCAAACAGGTGCTTGGTGTGCTGGTGGTGCAGCAAAAATCTCCCCGTTTAT TTAGCGAGATGGAAGTCTCCTTTTTAGTTACCCTTGCTGCGCAGCTCGCGGTGCTGGTGGCGCATGCTCAAACGCAAGGT CACTGGCGTTTATCCAAAAAGCAGCAGGCCATTACGGGTGTTGCCGCCTCTTCTGGGGTCGCGATTGGTGAGTTTTGGTG GGATGATACCCAGCCAGATCTGTCCGAAGTGCTACCCGCCTCAGCTCTGGATCCTGATTTTGAGCAAGAGCGTATTGCCT TGGCAGTAGAGAGTGCGCTGGCCGATTTTCGTCGGATGCGTAAAAAGCTCGATGGGGATATCAACAAAGAAGCGTTAGCC ATTTTTGATCTGTTCACTCACTTATTGAACGATCCTATGCTGCGGAAAGATCTCAAAGCGCAGATCCAAAAAGGGGATCG TGCCGATTGGGCGCTGCGCCAAGTGGTCGAAAGTTATTCGAACCGTTTTGCGCGCACGTCGGATGTTTATCTGCGAGAGC GAGCGCAAGACATTCGTGAGTTAGGACAACGTTTACTGTTTTTCTTGCTCAATACCGAAACCAATCAGCCCAAAATTGAT AAGCCGGTTATCTTGGTGGTGCGAGAGCTGACCGCGAGCGTGTTAGCCGCACTGCCCAAAGATAAGCTGCTGGCTGTCGT CTCTCTTGAAGGCGCGGCCAACTCCCATGCGGCTATTTTGTCGCGCGCGTTAGGCATTCCTGCGGTGATGGGGGTATCGA TTAACCTGCGCGATCTGAATGGCAAAAAAGGCATTGTCGATGGCTACAGCGGTAAGTTATTCATTTCGCCTTCGAAAACA ATTCTGAATGAGTATCGTGCCTTAGCGAATGAAGAGCGTGAGCTGTCGCGCATGGTCAATGAGGCGATTCGTGAGCCCGC ATGTACTCTTGATGGCGCACGAATCGAATTGTTACTGAATGCCGGTTTGAGTGCCGATACCAGTATTGCCGTCAATCAAG GGGTGGATGGCGTTGGGTTGTATCGCACAGAAATCTCTTTCTTACTGCAGCACCGATTTCCCTCAGAAGAGGAGCAGACT CAGCAATATCGGCATGTGCTGAACACTTACCCTCATCAACGTGTTGTTATGCGTACGCTCGATATCGGTGGTGATAAGCC TTTACCTTACTTGCCGATTGAAGAAGACAACCCATTTCTCGGTTGGCGTGGTATCCGTTTTACCTTAGATCATCCTGACA TTTTTATTATCCAACTGCGCGCCATGCTCAGAGCAAGTGCGGAGTCGGGGAATTTATCCATCTTGCTGCCGATGATTTCC GGTGCCAAAGAGCTGGATGATGCGCTGAAATTTATCTATCAAGCCTATCAAGAAGTTTCGCAACAAGATCCACGCGTGGT GATGCCGCAAATCGGGATCATGCTCGAAGTACCTTCGATGCTGTATCTGCTGCCACTGATTGCCGATAAAATTGATTTCG TCTCTGTCGGTACAAACGATTTGACCCAATATCTGCTTGCCGTCGATCGTAACAATGCGCGTGTGGCGGATGTGTATGAA TCTATGCATCCTGCGGTGGTGATGGCACTGAAGCAGATCCAACAGACTTGCGCGACCCATCAAATACCCGTGTGTGTCTG TGGTGAGTTAGCCGGAGATCCGATTGGTGCTTTGCTGTTGATAGGGTTAGGCTACACCACCTTAAGTATGAACACGTCTA ACGTCGCTAAAGTGAAATATCTGGTGCGACACTCTGAACTGGCGGAATTAACCCAGCTCGCTGAGCAGGCATTAACCCAA CCTTATGGACGAGAAATCTACAATATGATGCTCGCCTATATCGAAAAACACGGTTTTGCAGGTTTTGTCCGTGCAGGCAA AAAATAG
Upstream 100 bases:
>100_bases GTGATGTTTATCGCCGCGCAATGAAAGAGTTCGCCTCATTAGCGATGCCATTTAAAGAACGGAAAGTGAAGGGCAAACGC AACACTCACAGAGGTTAAGC
Downstream 100 bases:
>100_bases GAACCAAATGTGAATATTGAACTCTTAGCTATACTGCTGCTGTTAGGGGCAGTGGTGGGTGTGCTTGCTGGCTTATTGGG CATTGGTGGCGGCTTACTGG
Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain
Products: NA
Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]
Number of amino acids: Translated: 748; Mature: 748
Protein sequence:
>748_residues MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARTSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ PYGREIYNMMLAYIEKHGFAGFVRAGKK
Sequences:
>Translated_748_residues MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARTSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ PYGREIYNMMLAYIEKHGFAGFVRAGKK >Mature_748_residues MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKFKGDKIHIDFDEGLVGLVKRS AEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQVLGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQG HWRLSKKQQAITGVAASSGVAIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARTSDVYLRERAQDIRELGQRLLFFLLNTETNQPKID KPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAILSRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKT ILNEYRALANEERELSRMVNEAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLRAMLRASAESGNLSILLPMIS GAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSMLYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYE SMHPAVVMALKQIQQTCATHQIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ PYGREIYNMMLAYIEKHGFAGFVRAGKK
Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)
COG id: COG3605
COG function: function code T; Signal transduction protein containing GAF and PtsI domains
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 GAF domain [H]
Homologues:
Organism=Escherichia coli, GI1789193, Length=751, Percent_Identity=49.9334221038615, Blast_Score=735, Evalue=0.0, Organism=Escherichia coli, GI1788756, Length=581, Percent_Identity=35.9724612736661, Blast_Score=318, Evalue=1e-87, Organism=Escherichia coli, GI48994992, Length=507, Percent_Identity=33.5305719921105, Blast_Score=255, Evalue=7e-69, Organism=Escherichia coli, GI1788726, Length=617, Percent_Identity=30.1458670988655, Blast_Score=238, Evalue=1e-63, Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=23.0576441102757, Blast_Score=76, Evalue=7e-15, Organism=Escherichia coli, GI226510935, Length=183, Percent_Identity=28.4153005464481, Blast_Score=69, Evalue=1e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003018 - InterPro: IPR008279 - InterPro: IPR006318 - InterPro: IPR018274 - InterPro: IPR023151 - InterPro: IPR000121 - InterPro: IPR008731 - InterPro: IPR015813 [H]
Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]
EC number: =2.7.3.9 [H]
Molecular weight: Translated: 83538; Mature: 83538
Theoretical pI: Translated: 6.01; Mature: 6.01
Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKF CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE KGDKIHIDFDEGLVGLVKRSAEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQV CCCEEEEECCCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH LGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQGHWRLSKKQQAITGVAASSGV HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCC AIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA EEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARTSDVYLRERAQDIRE HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH LGQRLLFFLLNTETNQPKIDKPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAIL HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH SRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKTILNEYRALANEERELSRMVN HHHHCCCEEEECEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHH EAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT HHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHH QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLR HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCEEHHHHH AMLRASAESGNLSILLPMISGAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSM HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHH LYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYESMHPAVVMALKQIQQTCATH HHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC QIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ CCCCEEECCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC PYGREIYNMMLAYIEKHGFAGFVRAGKK HHHHHHHHHHHHHHHHCCCHHHHCCCCC >Mature Secondary Structure MLSQLRDIVEQVSKVEDVYQALDIFVKQTCEAMSTECCTVYLANEEMHRLELMATQGLKF CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCEE KGDKIHIDFDEGLVGLVKRSAEPINLAEASKHPSFKYFKQLGEEVYHSFLGTPIIYRKQV CCCEEEEECCCCHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH LGVLVVQQKSPRLFSEMEVSFLVTLAAQLAVLVAHAQTQGHWRLSKKQQAITGVAASSGV HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCC AIGEFWWDDTQPDLSEVLPASALDPDFEQERIALAVESALADFRRMRKKLDGDINKEALA EEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH IFDLFTHLLNDPMLRKDLKAQIQKGDRADWALRQVVESYSNRFARTSDVYLRERAQDIRE HHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH LGQRLLFFLLNTETNQPKIDKPVILVVRELTASVLAALPKDKLLAVVSLEGAANSHAAIL HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHH SRALGIPAVMGVSINLRDLNGKKGIVDGYSGKLFISPSKTILNEYRALANEERELSRMVN HHHHCCCEEEECEEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHH EAIREPACTLDGARIELLLNAGLSADTSIAVNQGVDGVGLYRTEISFLLQHRFPSEEEQT HHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHH QQYRHVLNTYPHQRVVMRTLDIGGDKPLPYLPIEEDNPFLGWRGIRFTLDHPDIFIIQLR HHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCEEHHHHH AMLRASAESGNLSILLPMISGAKELDDALKFIYQAYQEVSQQDPRVVMPQIGIMLEVPSM HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEHHHH LYLLPLIADKIDFVSVGTNDLTQYLLAVDRNNARVADVYESMHPAVVMALKQIQQTCATH HHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC QIPVCVCGELAGDPIGALLLIGLGYTTLSMNTSNVAKVKYLVRHSELAELTQLAEQALTQ CCCCEEECCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC PYGREIYNMMLAYIEKHGFAGFVRAGKK HHHHHHHHHHHHHHHHCCCHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9278503; 7896715; 8973315 [H]