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The map label for this gene is yfiC [C]

Identifier: 227080842

GI number: 227080842

Start: 664394

End: 665116

Strand: Reverse

Name: yfiC [C]

Synonym: VCM66_0619

Alternate gene names: 227080842

Gene position: 665116-664394 (Counterclockwise)

Preceding gene: 227080848

Following gene: 227080841

Centisome position: 22.99

GC content: 48.82

Gene sequence:

>723_bases
ATGAAAACCAATAAATTAATCAATAAAAGCTTTCGCTTTAAACAATTCTCTATAGAAGAAGGCACATGCGGCATGCCAAT
CAGTACCGATGGCGTTTTACTCGGTAGCTGGGCATTTAGCCTCTCTCCAACGACGATTTTAGATATTGGCTGTGGTACAG
GGCTTTTGAGTTTAATGTGCGCGCAGCGCTTCCCTCATGCCCACATCACCGCTTTGGATATTGAGCAAACTGCATATCTA
GCGGCAGAGCACAATCGGCAGCAGAGCCCTTGGGCTGAACGCATCGAATGCCAACATGCCGATATTCTGCACTGGCAGCC
CAGCAAACGCTTTGCCGCAATCATTTGTAATCCTCCTTATTTCAACAGCGGAGAAACCGCTCAACACCAAGTCAGAGCGA
CGGCACGCCACACTATCAGCTTGCAGCACCAAGCCTTGATTGAGCGCTTACCTCAGCTTCTCGAACCCGATGGCGTAGCG
AGTTTTATTCTGCCTAAAGCGGAAGGTGAAGATTTCATCGCCCTAGCCAGACAAGCTGGGCTCTTTGTGGGGCGCTATTG
TCAGGTTCAACCAACCACGGACAAACCTGTGCATCGTCTATTATTTGAGCTGCACCTATCCCCTTGTTTACCAGTAGAGA
CAAGACTGGTGATCCGTGAGCAGCAGGGTTACAGTGAGGCGTTTTGCCAGTTAACTCGCGATTTTTATCTGAAGATGTCG
TAA

Upstream 100 bases:

>100_bases
CTTTATAAGCTCATTTTTTCACCAGTCAACAGAATAAAATATTATTAACTATCTTCAGAAGAGAGCTTTTCGCTTAACTC
TCGTTTGACCTAGAATCTCG

Downstream 100 bases:

>100_bases
TTAAAGATGTGATCTAAAGCGTGATCCATCTATAATGTGCGCCCTGTTTTAAGACAGAGCCTTTCAACGCTTGTGGAGTA
ATTATTTGTGATTAAAACCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQTAYL
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVA
SFILPKAEGEDFIALARQAGLFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS

Sequences:

>Translated_240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQTAYL
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVA
SFILPKAEGEDFIALARQAGLFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
>Mature_240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQTAYL
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVA
SFILPKAEGEDFIALARQAGLFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val)

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family

Homologues:

Organism=Escherichia coli, GI87082131, Length=242, Percent_Identity=38.4297520661157, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMN6_VIBCH (Q9KU62)

Other databases:

- EMBL:   AE003852
- PIR:   G82295
- RefSeq:   NP_230310.1
- ProteinModelPortal:   Q9KU62
- GeneID:   2615451
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0661
- TIGR:   VC_0661
- HOGENOM:   HBG307609
- OMA:   FKQFHIN
- ProtClustDB:   CLSK874062
- GO:   GO:0005737
- HAMAP:   MF_01872
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR007848
- InterPro:   IPR022882
- PRINTS:   PR00507

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.55

Molecular weight: Translated: 27049; Mature: 27049

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMC
CCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
AQRFPHAHITALDIEQTAYLAAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPY
HHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEEECCCC
FNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVASFILPKAEGEDFIALARQAG
CCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
LFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
CEEECEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMC
CCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
AQRFPHAHITALDIEQTAYLAAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPY
HHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEEECCCC
FNSGETAQHQVRATARHTISLQHQALIERLPQLLEPDGVASFILPKAEGEDFIALARQAG
CCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
LFVGRYCQVQPTTDKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
CEEECEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301