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The map label for this gene is pnp [H]

Identifier: 227080828

GI number: 227080828

Start: 647693

End: 649822

Strand: Direct

Name: pnp [H]

Synonym: VCM66_0605

Alternate gene names: 227080828

Gene position: 647693-649822 (Clockwise)

Preceding gene: 227080827

Following gene: 227080829

Centisome position: 22.39

GC content: 49.77

Gene sequence:

>2130_bases
ATGTTCGAAAAACCAGTAGTAAAAACGTTTCAGTACGGTAACCACACCGTTACTCTCGAAACGGGTGTAATGGCTCGCCA
AGCAACGGCAGCAGTAATGGCTACCATGGATGACACTGCGGTGTTTGTCTCTGTGGTCGGTAAGAAAGAAGCGGTAGTGG
GTCAAGATTTCTTCCCTCTGACTGTAAACTACCAAGAGCGTACTTACGCGGCAGGTAAAATCCCTGGCGGTTTCTTTAAG
CGCGAAGGTCGTCCTTCTGAAGGCGAAACGCTGATTGCTCGTCTGATTGACCGTCCAATTCGTCCGCTGTTCCCTGATGG
TTTCACTAACGAAGTGCAAGTGATCGCGACTGTGGTTTCAGTAAACCCAGACGTGCAACCTGACATCATCTCGATGATCG
GTACTTCTGCAGCACTGGCGATTTCTGGACTCCCATTCAATGGTCCAATCGGTGCGGCTCGCGTAGGTCACATTGATGGT
CAATTGGTTCTGAACCCAAGTGAAAAAGAGCTGAAACAGTCTCGTTTGGATCTGGTGGTTGCGGGTACTGACAACGCAGT
ACTGATGGTGGAATCAGAAGCACAAATTCTGACTGAAGAAGAGATGCTGGCTGCCGTTGTGTTTGGTCACGATCAACAAC
AAGCCGTGATCAAAGCAATCAATGAATTCGCAGCGGAAGTTGCAACACCAGCATGGGAATGGGTTGCACCAGCCGAGAAC
ACTGAGCTGAAAGCGAAAGTGGCTGCTCTGGCAGAAACTCGTTTGGTGGAAGCGTACCAAATCACTGAGAAAATGGCGCG
TTATGATCGTATCCATGAAATCAGTGCAGAAGTCACGGCGGCTCTGCTGGCTGAAAACGAAGCTTTAGACACCAAAGAAA
TTCACACCATTTTCCACGATCTGGAAAAAACCGTTGTTCGTCGCAGCATCATCGCGGGTAACCCACGTATCGATGGCCGT
GAAAAAGACATGGTTCGTGCTCTGGACGTTCGTACTGGCGTTCTGCCACGTACTCACGGCTCTGCTCTGTTCACACGTGG
TGAAACTCAGGCTCTGGTTACCGCAACTCTAGGCACTCAGCGTGATGCGCAAATCATCGATGAGCTGACTGGCGAGAAGA
AAGATCACTTCCTACTGCACTACAACTTCCCTCCATACTGTGTGGGTGAAACGGGTTTTGTGGGTTCTCCTAAGCGTCGC
GAAATCGGTCACGGCCGTTTGGCGAAGCGTGGTATTGCTGCGGTTATGCCATCACCAGAAGAGTTCCCGTACACAGTACG
TGTCGTTTCTGAAATCACGGAATCTAACGGTTCATCTTCAATGGCTTCTGTGTGTGGTTCATCGCTTGCTCTGATGGATG
CCGGTGTTCCAATCAAGGCTTCTGTTGCGGGTATCGCAATGGGTCTGGTGAAAGAAGAAAATGATTTCGTGGTTCTGTCT
GACATCCTAGGTGATGAAGATCACCTTGGCGACATGGACTTTAAAGTCGCGGGTACCGCGACAGGTGTTACTGCACTGCA
GATGGACATCAAGATCGAAGGTATCACCAAAGAGATCATGCAAATCGCTCTGAACCAAGCGAAAGGTGCTCGTCTACACA
TTCTGTCTGTGATGGATCAAGCGATCTCTGCAGCGCGTAGCGATATCTCTGAATTTGCGCCACGTATCCACACGATGAAG
ATCAGCGTTGAGAAGATCAAAGACGTGATCGGTAAAGGCGGTGCGGTAATTCGTCAGTTGACGGAAGAAACGGGTACCAC
CATCGAGATCGAAGATGACGGCACGATCAAGATTGCTGCGACTGACGGCGATCAAGCGAAAGAAGCGATCCGCCGTATTC
AAGAGATCACCGCCGAAGTTGAAGTGGGCGTGATCTACACTGGTAAAGTAGCTCGTTTGGCAGACTTTGGTGCGTTTGTA
ACGATTCTTCCTGGTAAAGATGGTCTAGTACACATCTCTCAAATCGCTGACAAGCGCGTAGAGAAAGTGTCTGACTACCT
GACTGAAGGCCAAGAAGTTCAAGTTAAAGTGCTGGAAATCGACCGCCAAGGCCGTGTACGTCTAAGCATGAAAGAAGCGG
TTGAAACTTCTGATGCTGCAGCAGCAGAAGTGGCACCACAAGCGGAATAA

Upstream 100 bases:

>100_bases
GTACTCCCAAGTTGTCAGTCTATTTTTACTAGTCGCGATTAGTAGTGCTCTGAGTATTAATGATTTGATTTCAGGCTTGT
CCTGAGAATAAGGAATACTC

Downstream 100 bases:

>100_bases
TTTTCCGTTGCTGGAGCAAAAACAACACGGCTTTTACTTTTCATAATAAAAGCGTGCTATAAAGGGAGCGTTATTCGCTC
CCTTTTTTATGCGAAATCCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE

Sequences:

>Translated_709_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE
>Mature_709_residues
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPLTVNYQERTYAAGKIPGGFFK
REGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVSVNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDG
QLVLNPSEKELKQSRLDLVVAGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHDLEKTVVRRSIIAGNPRIDGR
EKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQRDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRR
EIGHGRLAKRGIAAVMPSPEEFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQAISAARSDISEFAPRIHTMK
ISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAATDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFV
TILPGKDGLVHISQIADKRVEKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=37.8186968838527, Blast_Score=441, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=76.8011527377522, Blast_Score=1105, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=666, Percent_Identity=32.7327327327327, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17535281, Length=80, Percent_Identity=46.25, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281362905, Length=714, Percent_Identity=38.375350140056, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651641, Length=714, Percent_Identity=38.375350140056, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24651643, Length=714, Percent_Identity=38.375350140056, Blast_Score=467, Evalue=1e-131,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=38.5321100917431, Blast_Score=431, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76586; Mature: 76586

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCCEEEEEECCCCCEEECCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
VNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDGQLVLNPSEKELKQSRLDLVV
CCCCCCHHHHHHHCCCHHEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHCCCCEEE
AGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
ECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGIAAVMPSPE
CHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHHHHH
AISAARSDISEFAPRIHTMKISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAA
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCCEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure
MFEKPVVKTFQYGNHTVTLETGVMARQATAAVMATMDDTAVFVSVVGKKEAVVGQDFFPL
CCCCCCHHHEECCCEEEEEECCCHHHHHHHHHEEECCCCEEEEEECCCCCEEECCCCEEE
TVNYQERTYAAGKIPGGFFKREGRPSEGETLIARLIDRPIRPLFPDGFTNEVQVIATVVS
EEECCCCEEECCCCCCCHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEE
VNPDVQPDIISMIGTSAALAISGLPFNGPIGAARVGHIDGQLVLNPSEKELKQSRLDLVV
CCCCCCHHHHHHHCCCHHEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHCCCCEEE
AGTDNAVLMVESEAQILTEEEMLAAVVFGHDQQQAVIKAINEFAAEVATPAWEWVAPAEN
ECCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCC
TELKAKVAALAETRLVEAYQITEKMARYDRIHEISAEVTAALLAENEALDTKEIHTIFHD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LEKTVVRRSIIAGNPRIDGREKDMVRALDVRTGVLPRTHGSALFTRGETQALVTATLGTQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEEECCCC
RDAQIIDELTGEKKDHFLLHYNFPPYCVGETGFVGSPKRREIGHGRLAKRGIAAVMPSPE
CHHHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTVRVVSEITESNGSSSMASVCGSSLALMDAGVPIKASVAGIAMGLVKEENDFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHEECCCCEEEEH
DILGDEDHLGDMDFKVAGTATGVTALQMDIKIEGITKEIMQIALNQAKGARLHILSVMDQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCEEHHHHHHHH
AISAARSDISEFAPRIHTMKISVEKIKDVIGKGGAVIRQLTEETGTTIEIEDDGTIKIAA
HHHHHHHHHHHHCCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEEE
TDGDQAKEAIRRIQEITAEVEVGVIYTGKVARLADFGAFVTILPGKDGLVHISQIADKRV
CCCHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCCEEEEECCCCCCEEHHHHHHHHH
EKVSDYLTEGQEVQVKVLEIDRQGRVRLSMKEAVETSDAAAAEVAPQAE
HHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA