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The map label for this gene is rpoS

Identifier: 227080717

GI number: 227080717

Start: 522179

End: 523186

Strand: Direct

Name: rpoS

Synonym: VCM66_0492

Alternate gene names: 227080717

Gene position: 522179-523186 (Clockwise)

Preceding gene: 227080716

Following gene: 227080719

Centisome position: 18.05

GC content: 46.83

Gene sequence:

>1008_bases
ATGAGTGTCAGCAATACCGTAACCAAAGTAGAAGAGTTCGATTTTGAAGATGAAGCACTGGAAGTGCTAGAAACTGATGC
CGAGCTCACCAGTGATGAAGAATTAGTTGCTGTTGAAGGGGCAAGTGAAGACGTTCGTGAAGAGTTTGATGCTTCTGCGA
AAAGTCTTGATGCGACCCAGATGTATCTCAGCGAAATTGGTTTTTCACCGCTCCTTACTGCCGAAGAAGAAGTGCTTTAT
GCTCGTCGTGCCTTACGTGGTGATGAAGCCGCACGTAAACGCATGATTGAAAGTAACTTGCGTCTGGTGGTAAAAATTTC
ACGCCGTTACAGCAACCGAGGATTAGCACTGCTCGATCTGATTGAAGAAGGTAATCTTGGTCTGATCCGTGCGGTTGAGA
AATTCGATCCAGAACGCGGTTTCCGCTTCTCTACCTACGCAACATGGTGGATCCGTCAAACCATTGAACGTGCGCTGATG
AACCAAACACGCACCATTCGTCTACCGATTCATGTCGTCAAAGAGCTGAACATTTATTTGCGTACTGCTCGCGAATTATC
ACAGCGCCTTGACCACGAACCTACACCAGAAGAAATTGCTCTTGAGTTAGACCGACCTGTCGATGATGTCACTAAGATGC
TGCGTCTTAACGAACGGATCAGCTCAGTGGATACGCCAATTGGTGGGGATGGAGATAAGGCACTGCTGGATATTTTGCCA
GACTCTCACAATGCCGATCCTGAGTTTTCAACTCAAGATGATGACATTCGTGAATCGCTGCTCAACTGGTTGGATGAACT
TAATCCAAAGCAAAAAGAAGTGCTTGCGCGTCGCTTTGGGCTTCTTGGCTATGAACCATCGACCTTGGAAGAAGTGGGTC
GTGAGATCAATCTCACTCGTGAGCGTGTTCGCCAAATCCAAGTGGAAGGTCTACGTCGTCTGCGTGAGATTTTGGTGAAA
CAAGGTTTGAATATGGAAGCGCTGTTTAACGTCGAATACGACAACTAA

Upstream 100 bases:

>100_bases
GTGAATCCTAAACGCTACTTACCTTAACTCATATTGCTAAGGTCATACTGCAAAGGTTGTAAATCGCAATGACTCGCAAA
GTTGCCAGGGGGAGGCCGCT

Downstream 100 bases:

>100_bases
TTTTTCCAGACTCATCCAAAACTAAGGCACCCGGTGGGGTGCCTTGTTGTTTCTGGAATCTGTAGATTCCATGCTTGAGA
TATGGGCAATAACTAGAGCA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: Sigma-38 factor

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFNVEYDN

Sequences:

>Translated_335_residues
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLY
ARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALM
NQTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVK
QGLNMEALFNVEYDN
>Mature_334_residues
SVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQMYLSEIGFSPLLTAEEEVLYA
RRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALMN
QTRTIRLPIHVVKELNIYLRTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILPD
SHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTRERVRQIQVEGLRRLREILVKQ
GLNMEALFNVEYDN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. May be required for the persistence of V.cholerae in aquatic habitats

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family

Homologues:

Organism=Escherichia coli, GI1789098, Length=323, Percent_Identity=73.0650154798762, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=45.3333333333333, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=29.2592592592593, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): RPOS_VIBCH (O51804)

Other databases:

- EMBL:   AF000945
- EMBL:   AE003852
- PIR:   A82312
- RefSeq:   NP_230185.1
- ProteinModelPortal:   O51804
- SMR:   O51804
- GeneID:   2615203
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0534
- TIGR:   VC_0534
- HOGENOM:   HBG745096
- OMA:   DNGPEDT
- ProtClustDB:   CLSK874000
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02394
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 38535; Mature: 38404

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
MYLSEIGFSPLLTAEEEVLYARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDL
HHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHCCCCEEEEHH
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALMNQTRTIRLPIHVVKELNIYL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
RTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
HHHHHHHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECC
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ERVRQIQVEGLRRLREILVKQGLNMEALFNVEYDN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
>Mature Secondary Structure 
SVSNTVTKVEEFDFEDEALEVLETDAELTSDEELVAVEGASEDVREEFDASAKSLDATQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
MYLSEIGFSPLLTAEEEVLYARRALRGDEAARKRMIESNLRLVVKISRRYSNRGLALLDL
HHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEHHHHCCCCEEEEHH
IEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERALMNQTRTIRLPIHVVKELNIYL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
RTARELSQRLDHEPTPEEIALELDRPVDDVTKMLRLNERISSVDTPIGGDGDKALLDILP
HHHHHHHHHCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECC
DSHNADPEFSTQDDDIRESLLNWLDELNPKQKEVLARRFGLLGYEPSTLEEVGREINLTR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ERVRQIQVEGLRRLREILVKQGLNMEALFNVEYDN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473029; 10952301