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The map label for this gene is folK-1 [H]

Identifier: 227080708

GI number: 227080708

Start: 515728

End: 516315

Strand: Direct

Name: folK-1 [H]

Synonym: VCM66_0483

Alternate gene names: 227080708

Gene position: 515728-516315 (Clockwise)

Preceding gene: 227080707

Following gene: 227080709

Centisome position: 17.83

GC content: 51.19

Gene sequence:

>588_bases
TTGGCTCCGCATCCGCTTGACCAAGCCGGGCGCAGTGCCGCAAGCCAAAGGAGTCGGCGTGATCATCGAGCGAGCGCGTG
GATGATCACCGCTTATATTGGTGTGGGCTCAAATCAGGATCGCCATCAGTCGATTGAAGCTGGCCTTCGTGCATTAGCCG
AGCTGGCGAGCCAGTGGCGTCTGTCCACGATTTATGAGTGTCCATCCGTTGGGTTTGCCAGCCATCCTTTCTTCAATCTG
ATGGTTGAGATGGAGACAGAGCTCGCGCTGCCTGAATTGATGAAGCAGTTAAAAGCGATCGAGCTGCGTTGTGGCCGTTT
GCCGAATGCACAAAAGTATCAGGATCGAACACTGGATCTTGATATTGTGCTGTATGGCGATTGTGTATCGGAGAATGATC
CAGTGCTGCCTCGACCGGATATCTATCGCTATCCGTTTGTGATTCAACCTTTATATGAGCTCTGTCCGCAGCGAGTGATT
GCTGGTGATGGTCGAACTGTTGAGCAGATTTGGCAACAAGCGCAGGATTTAGCATCACTCACCCCAGTACCGATCTGGTT
TTCTATTAACGAGAAGAAACAATCATGA

Upstream 100 bases:

>100_bases
GCCAAGCTGTGCTGGAGCATATCGAACAAGGGCGTTTTTTACTGGTAGAGCGTGTGGCTGAAGAAGTGGCTGAACTGATC
ATGACTCGCTTTGCTGTGCC

Downstream 100 bases:

>100_bases
GTTATTTTGAGTCGTTCATTCTGGCTTTAATTCAAGGCTTTACCGAGTTTTTACCGATTTCTAGCTCCGCACACCTGATC
TTGCCTTCGGCTATTTTAGG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MAPHPLDQAGRSAASQRSRRDHRASAWMITAYIGVGSNQDRHQSIEAGLRALAELASQWRLSTIYECPSVGFASHPFFNL
MVEMETELALPELMKQLKAIELRCGRLPNAQKYQDRTLDLDIVLYGDCVSENDPVLPRPDIYRYPFVIQPLYELCPQRVI
AGDGRTVEQIWQQAQDLASLTPVPIWFSINEKKQS

Sequences:

>Translated_195_residues
MAPHPLDQAGRSAASQRSRRDHRASAWMITAYIGVGSNQDRHQSIEAGLRALAELASQWRLSTIYECPSVGFASHPFFNL
MVEMETELALPELMKQLKAIELRCGRLPNAQKYQDRTLDLDIVLYGDCVSENDPVLPRPDIYRYPFVIQPLYELCPQRVI
AGDGRTVEQIWQQAQDLASLTPVPIWFSINEKKQS
>Mature_194_residues
APHPLDQAGRSAASQRSRRDHRASAWMITAYIGVGSNQDRHQSIEAGLRALAELASQWRLSTIYECPSVGFASHPFFNLM
VEMETELALPELMKQLKAIELRCGRLPNAQKYQDRTLDLDIVLYGDCVSENDPVLPRPDIYRYPFVIQPLYELCPQRVIA
GDGRTVEQIWQQAQDLASLTPVPIWFSINEKKQS

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=148, Percent_Identity=35.8108108108108, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 22141; Mature: 22009

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPHPLDQAGRSAASQRSRRDHRASAWMITAYIGVGSNQDRHQSIEAGLRALAELASQWR
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LSTIYECPSVGFASHPFFNLMVEMETELALPELMKQLKAIELRCGRLPNAQKYQDRTLDL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEE
DIVLYGDCVSENDPVLPRPDIYRYPFVIQPLYELCPQRVIAGDGRTVEQIWQQAQDLASL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHC
TPVPIWFSINEKKQS
CCCEEEEEECCCCCC
>Mature Secondary Structure 
APHPLDQAGRSAASQRSRRDHRASAWMITAYIGVGSNQDRHQSIEAGLRALAELASQWR
CCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LSTIYECPSVGFASHPFFNLMVEMETELALPELMKQLKAIELRCGRLPNAQKYQDRTLDL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEE
DIVLYGDCVSENDPVLPRPDIYRYPFVIQPLYELCPQRVIAGDGRTVEQIWQQAQDLASL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHC
TPVPIWFSINEKKQS
CCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1325970; 8202364; 9278503; 1657875; 2537812; 10378268; 10452528 [H]