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The map label for this gene is mscS [H]

Identifier: 227080690

GI number: 227080690

Start: 495546

End: 496409

Strand: Direct

Name: mscS [H]

Synonym: VCM66_0465

Alternate gene names: 227080690

Gene position: 495546-496409 (Clockwise)

Preceding gene: 227080689

Following gene: 227080692

Centisome position: 17.13

GC content: 45.25

Gene sequence:

>864_bases
ATGGCTGGTGAATCGATTGGTGTTGAAGTGCCTATCGTCGAAAGTTTTAACCAAGTGAATACTTGGTTAACCAATAATTC
GGATCTTTTGATTCAGTACGGCGTGAATGTGATTTCCGCTATTCTGATCCTATTTATCGGTAACCTTGTGGTTAAAGGGG
TTGCAGGTAGTGTGGCGAATGTATTGAAAAAGAAAGAGATGGATAAGGCTGTCGTAGACTTTATCCACGGTCTTGTTCGT
TACACGCTGTTTATTATTGTGTTGATTGCGGCGTTGAGTCGTATTGGTGTGCAAACTGCGTCGGTTGTCGCTGTGATTGG
TGCTGCGGGTCTGGCCGTTGGTTTGGCTCTACAGGGTTCGCTGTCTAATTTTGCAGCCGGTGTACTGATTGTGGCATTCC
GCCCATTCAAATCCGGTGATTATGTAGAAATTGGTGGCGTCGCCGGCTCGGTTGATTCTATCCAAATTTTCCAAACTGTC
CTGAAAAGCCCAGATAATAAAATGGTTGTGGTACCAAACTCGGCGGTCATTGGCGGTGCTATTACTAACTATTCACGTCA
CGAAACGCGTCGTGTCGACATGGTGATTGGGGTTTCTTACAAGTCTGATCTGCAGAAAACCAAACGTGTTCTGCGTGAGA
CGCTGGAAAAAGATCCTCGTATCCTCAAAGATCCGGATATGACAATTGGTGTGCTGACGCTGGCGGATTCATCAATCAAC
TTTGTGGTTCGTCCATGGTGTAAAACTTCGGACTATTGGGCAGTGTACTTTGATTCGATGCAAGCGATCAAAGAAGCGCT
GGATGCCAACGGTATCGAAATTCCATTCCCACAAATGGATGTGCATCTGAACAAAATCAATTAA

Upstream 100 bases:

>100_bases
TTTTGCCAAAATTCTGACTTTTGGTGGTTTATTTGAGTGGGATGTTTATCATTTCTCATTATTTCCGCTAATTTTTGATT
ACTTGTAAGAGGAATGCACT

Downstream 100 bases:

>100_bases
TGGTTTTGCTATATACAAAATACCAAAGCGGAGTGTTTACTCCGCTTTTTTGTCGGTAATAGCTTAATTTAACCAGTGAT
TGAGATATAAGTTCTTAGTC

Product: Small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAGESIGVEVPIVESFNQVNTWLTNNSDLLIQYGVNVISAILILFIGNLVVKGVAGSVANVLKKKEMDKAVVDFIHGLVR
YTLFIIVLIAALSRIGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTV
LKSPDNKMVVVPNSAVIGGAITNYSRHETRRVDMVIGVSYKSDLQKTKRVLRETLEKDPRILKDPDMTIGVLTLADSSIN
FVVRPWCKTSDYWAVYFDSMQAIKEALDANGIEIPFPQMDVHLNKIN

Sequences:

>Translated_287_residues
MAGESIGVEVPIVESFNQVNTWLTNNSDLLIQYGVNVISAILILFIGNLVVKGVAGSVANVLKKKEMDKAVVDFIHGLVR
YTLFIIVLIAALSRIGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTV
LKSPDNKMVVVPNSAVIGGAITNYSRHETRRVDMVIGVSYKSDLQKTKRVLRETLEKDPRILKDPDMTIGVLTLADSSIN
FVVRPWCKTSDYWAVYFDSMQAIKEALDANGIEIPFPQMDVHLNKIN
>Mature_286_residues
AGESIGVEVPIVESFNQVNTWLTNNSDLLIQYGVNVISAILILFIGNLVVKGVAGSVANVLKKKEMDKAVVDFIHGLVRY
TLFIIVLIAALSRIGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTVL
KSPDNKMVVVPNSAVIGGAITNYSRHETRRVDMVIGVSYKSDLQKTKRVLRETLEKDPRILKDPDMTIGVLTLADSSINF
VVRPWCKTSDYWAVYFDSMQAIKEALDANGIEIPFPQMDVHLNKIN

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=278, Percent_Identity=47.841726618705, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1786670, Length=206, Percent_Identity=28.6407766990291, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787591, Length=233, Percent_Identity=26.6094420600858, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367355, Length=254, Percent_Identity=23.6220472440945, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 31009; Mature: 30877

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGESIGVEVPIVESFNQVNTWLTNNSDLLIQYGVNVISAILILFIGNLVVKGVAGSVAN
CCCCCCCEEECHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKKKEMDKAVVDFIHGLVRYTLFIIVLIAALSRIGVQTASVVAVIGAAGLAVGLALQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTVLKSPDNKMVVVPNSAVIGGA
HHHHHHEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEECCH
ITNYSRHETRRVDMVIGVSYKSDLQKTKRVLRETLEKDPRILKDPDMTIGVLTLADSSIN
HCCCCHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCC
FVVRPWCKTSDYWAVYFDSMQAIKEALDANGIEIPFPQMDVHLNKIN
EEEECCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
AGESIGVEVPIVESFNQVNTWLTNNSDLLIQYGVNVISAILILFIGNLVVKGVAGSVAN
CCCCCCEEECHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKKKEMDKAVVDFIHGLVRYTLFIIVLIAALSRIGVQTASVVAVIGAAGLAVGLALQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
LSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVDSIQIFQTVLKSPDNKMVVVPNSAVIGGA
HHHHHHEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEECCH
ITNYSRHETRRVDMVIGVSYKSDLQKTKRVLRETLEKDPRILKDPDMTIGVLTLADSSIN
HCCCCHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCC
FVVRPWCKTSDYWAVYFDSMQAIKEALDANGIEIPFPQMDVHLNKIN
EEEECCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]