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The map label for this gene is pilT-2 [H]

Identifier: 227080673

GI number: 227080673

Start: 477117

End: 478223

Strand: Direct

Name: pilT-2 [H]

Synonym: VCM66_0448

Alternate gene names: 227080673

Gene position: 477117-478223 (Clockwise)

Preceding gene: 227080672

Following gene: 227080674

Centisome position: 16.49

GC content: 50.77

Gene sequence:

>1107_bases
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTCGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Upstream 100 bases:

>100_bases
AAAATGCCAAACAGCTGATGGCACGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCA
TTTTAGGTAGGTAAAGACTG

Downstream 100 bases:

>100_bases
CGATGGTTACTTTGTGCGCTTTGCTTTTACGCCCTGCAACTCAAGCGAATTTGGGATAGAGCTTCGAGTCTGGATTGGGC
ATACTGCCACTTTCTCGGCT

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Sequences:

>Translated_368_residues
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME
>Mature_368_residues
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=322, Percent_Identity=40.3726708074534, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1789723, Length=210, Percent_Identity=37.1428571428571, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786296, Length=279, Percent_Identity=31.5412186379928, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41308; Mature: 41308

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQA
CCHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
EFKQTREANFAVVRDSGRFRVSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAK
HHHHHCCCCEEEEECCCCEEEEEHHHHCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHC
RGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPIEFVHEHKRCIVTQREVGLDT
CCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHEEEHHHHCCCC
ESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHEL
HCCHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHHH
KATMARSQEVGMQTFDQALYQLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
QNVKIDME
EEEEEECC
>Mature Secondary Structure
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQA
CCHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
EFKQTREANFAVVRDSGRFRVSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAK
HHHHHCCCCEEEEECCCCEEEEEHHHHCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHC
RGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPIEFVHEHKRCIVTQREVGLDT
CCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHEEEHHHHCCCC
ESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
HHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHEL
HCCHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCHHHH
KATMARSQEVGMQTFDQALYQLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCC
QNVKIDME
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]