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The map label for this gene is glsA [H]

Identifier: 227080664

GI number: 227080664

Start: 470054

End: 471004

Strand: Direct

Name: glsA [H]

Synonym: VCM66_0439

Alternate gene names: 227080664

Gene position: 470054-471004 (Clockwise)

Preceding gene: 227080663

Following gene: 227080672

Centisome position: 16.25

GC content: 51.1

Gene sequence:

>951_bases
TTGAGACAAACTAATAATAAAGAGTCAACCATGAAGCCAACAGCAGACATTTTAGCCAGCATTATTGAAGAAGTTCGCCC
GCTCACCAGTAAAGGCATCGTCGCCGATTACATCCCCGCACTAGCGAAAGTACCGAGTGATAAATTGGGTATTGCTGTGT
TTACCAACCAAGGGGAAGTCATCACGGCAGGCGATGCACAGGAAGGGTTTTCGATTCAGTCGATATCCAAAGTGCTGAGC
TTGACGCTCGCGATGGGCTTGTATCAGCCAGATGAACTGTGGAGCCGCGTGGGTAAAGAGCCTTCAGGACAAGCATTTAA
CTCATTGATTCAGCTTGAAATGGAGCAGGGAATCCCACGTAATCCATTTATCAATGCCGGCGCGATTGTGGTGTGCGACA
TGCTGCAAAGTCGCCTCTCTGCACCTCGTCAGCGTCTGTTGGAGTTTGTACGCCAGCTCAGCGGCGAGCCGCAAATTGCT
TATGACAAAGTCGTGGCTGCTTCGGAGATGATGCACAGTGATCGCAACGCGGCGATTGCGTATTTGATGCGTTCATTTGG
CAATTTCCACAATGAGGTTATTCCGGTACTGCACAACTATTTTCACGCCTGCGCATTGAAAATGAGCTGTGTTGAGCTGG
CGAAAACCTTCAGCTATCTGGCCAACAAAGGTGTTTCTGTGGTGACGGGCGAAACGGTGATCACGCCAACCCAAAGCAAA
CAAACCAATGCCTTACTCGCCACGTGCGGCTTGTATGATGGGGCAGGAGAGTTTGCCTATCGAGTTGGTATGCCCGGAAA
ATCAGGTGTTGGCGGTGGGATTATTGCTGTGGTGCCGGGAGAAATGACGATTGCAGTGTGGTCCCCCGCGCTGGATCAAT
CGGGTAACTCGCTCGCGGGGACTCGCGCGTTAGAGTTACTCGCGCAGCGGATTGGTCGCTCAATTTTCTAA

Upstream 100 bases:

>100_bases
AGTACCAGCGCACTGCGTAAAGAGAGAATTTTGCTTAAGCCAACATTTGATGTTGGCTTTTTTGACCCTAATCCTCAAGC
TATTGAAAAGCTGAAGTAGC

Downstream 100 bases:

>100_bases
GCTATCCCCTTTCTAATTGAAGCTGCAGCGGTGTTGGCAGTATTCGCTCTCCCCAATCACATAGTGGATCTACGCTCATG
GGGATTATGAGAGCCATCCT

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRQTNNKESTMKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEVITAGDAQEGFSIQSISKVLS
LTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIA
YDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSK
QTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF

Sequences:

>Translated_316_residues
MRQTNNKESTMKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEVITAGDAQEGFSIQSISKVLS
LTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIA
YDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSK
QTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF
>Mature_316_residues
MRQTNNKESTMKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEVITAGDAQEGFSIQSISKVLS
LTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPRNPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIA
YDKVVAASEMMHSDRNAAIAYLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSK
QTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAGTRALELLAQRIGRSIF

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family [H]

Homologues:

Organism=Homo sapiens, GI156104878, Length=275, Percent_Identity=37.0909090909091, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI20336214, Length=285, Percent_Identity=35.4385964912281, Blast_Score=174, Evalue=7e-44,
Organism=Escherichia coli, GI1787804, Length=300, Percent_Identity=67, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1786693, Length=283, Percent_Identity=39.9293286219081, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17507019, Length=299, Percent_Identity=38.1270903010033, Blast_Score=198, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17532727, Length=292, Percent_Identity=33.9041095890411, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI193204073, Length=293, Percent_Identity=35.8361774744027, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI193204075, Length=295, Percent_Identity=35.5932203389831, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24653166, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI281363241, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24653162, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24653164, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24653156, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI116008307, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24653158, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI281363239, Length=285, Percent_Identity=35.4385964912281, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015868 [H]

Pfam domain/function: PF04960 Glutaminase [H]

EC number: =3.5.1.2 [H]

Molecular weight: Translated: 33956; Mature: 33956

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTNNKESTMKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEV
CCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCE
ITAGDAQEGFSIQSISKVLSLTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPR
EECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
NPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIAYDKVVAASEMMHSDRNAAIA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
YLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
QTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAG
CCCHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHH
TRALELLAQRIGRSIF
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRQTNNKESTMKPTADILASIIEEVRPLTSKGIVADYIPALAKVPSDKLGIAVFTNQGEV
CCCCCCCCCCCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCE
ITAGDAQEGFSIQSISKVLSLTLAMGLYQPDELWSRVGKEPSGQAFNSLIQLEMEQGIPR
EECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
NPFINAGAIVVCDMLQSRLSAPRQRLLEFVRQLSGEPQIAYDKVVAASEMMHSDRNAAIA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
YLMRSFGNFHNEVIPVLHNYFHACALKMSCVELAKTFSYLANKGVSVVTGETVITPTQSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
QTNALLATCGLYDGAGEFAYRVGMPGKSGVGGGIIAVVPGEMTIAVWSPALDQSGNSLAG
CCCHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCHHH
TRALELLAQRIGRSIF
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA