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The map label for this gene is mshH [H]

Identifier: 227080609

GI number: 227080609

Start: 407989

End: 410010

Strand: Direct

Name: mshH [H]

Synonym: VCM66_0383

Alternate gene names: 227080609

Gene position: 407989-410010 (Clockwise)

Preceding gene: 227080608

Following gene: 227080610

Centisome position: 14.1

GC content: 48.42

Gene sequence:

>2022_bases
GTGGCACAAATGAGGTATACACCGACATTAAAACTGAGCACTCGGTTAGTGGCGTTCGTCACTATGACGGTGATCAGTGC
CATGTTTATCCTGTTCCTTGGTGGCACCTTATCTTTTCAGCGTTTGGGGCAGGAATACCTCAACCACTATCTACAAGGGA
TAGTTGAGGTTGTCGATAAGGAGATGGAAGATCCAGACGCCGCTTACTCTATGCAGCGTTGGATGCCCAAAATGCTTCAA
GCGAGTGGCATCATTGAAATGCAGCTTACTTCCGAAGCGGGCCCCATCTACCGTTTTAAAGACACCACCAACACCATTGA
AGCTTCACGTTTGCATCAAGTCGCTTTACCGCTCAAACGTAATCCCGGTTATGTGATGCATTTTAAAGCGGTGCCTCCCT
ACCTCAATTACAACTATTCACTGCAAGCCATGTGGTCGACCACCTTAGCGGTGCTACTGATCGTATTTTTCTTGATCCGC
GGTCTGAAATGGTTGAAAGAGCAACTACTAGGATCGGAGTTACTGGAAGAGCGAGGACGGATGATTCTTGCTGGACGAGT
GGAGCAACATGCCAAGGGGGATCCGCGAGAGTGGCCTTTCACCGCAAGTGAAGCCTTAGATCGCTTGATTGAAGAGTTGC
AAGATGCCCGTCAAGAACGTAGCCGTTTTGATACCTTTATTCGTAGCCAAACCTTCCTCGATCAACTGACCGGGACGGCG
AATCGGGTGTTGTATGACAGTAAGCTGGAATCGGCTTTACTGGAGAGTGGCGCTCACGGCGGCGTTATGATGCTGCGGGT
CGATGACTTAGAGTCAGCGCGAGAAGAGAGCCCTAAACGTACGCTCGATGAGTTTATCATTGAGGTGGGGGAGTGTTTAT
CCAACATAGTACAGCGTTATCCGGATGCCATACTTTCTCGCTACTATGAGGATGTGTTTGCGCTGTTTATTCCTCATCAG
GGTAGTAAAGATATTGCTCAAGTAGCGACACAGGCGATTAAGTTGATTGAGCGCATCAATCCCCCTGAGCCACTGCCAGA
AGACAATTGGTTCCACATTGGTGTAACCATGTATCAAGAAGGTGAGCGGCGTGGACGGATTATTGATGAGATGGAAACCG
CGCTCAAAAGTGCTCAACTGCAAGGGGTGAATGCGTGGAGCCGTTTTCAAAAACCTAAACAGTTACAAGAGGATCGCGGT
AGCGTTCGGTGGCGTACATTGTTTGAACAGGTGCTTCGTCCGGAAGAGATTTTGCTCTATCGCCAAGCCTGTTATCGTAT
TTCTGCTAATGGAGAGCGGGAGTTCCTACATTATGAGTTATTTGCGCGTATTCAGGATCCGCAACAAGGCATTCTCAAAG
CGTCGCGTTTTAGCTCAGCTTTAGAAACGGTCGGCTATGAAGCCATGTTGGATCGTGCCGTATTCAGCTCTGTCGTGAGT
TTTTTGAAGCGCTCTGAACTGGTTGAACCACTCTCGGTAAACTTGCACGTTGTTCCATTTAACGATAAACGGTATGCTCG
CTGGATTCGCAACGAATTGATGCAGATGCCTTTTTCTCTTAGAACGGCGCTCTCTTTTGAGTTTTCAGAAGCGCATTTGG
TCAACCATTTAGACTATATGCGCCCCATCATTCGCATGTTGGCGGGGCTGGGTTGCAAGATAGTGGTGGGACAAGCGGGA
CGAACAATTGTCAGCACGCACTATATCAAAGACTTAAAAGTCAACTATCTTAAACTTCATCGGAGTTTGATAAAGAAAAT
TGACCAACGCCATGAAAATCAGCTGTTCGTTCGCAGTTTGGTAGGTGTATGTGGCGGTACCCAAGCAGAAGTGGTTGCGG
TTGGGGTTGAAACTACTCAAGAGTGGCAAACCTTACAGATGCTGGGTGTCAGTGGTGTGCAAGGTCGCTTGTTTGATGAG
GAGCAACAGATGTTGCCTCCTTTGCCACCAGAGCCATTGAAACGCCCCCCGCGTCTCTCTGTAAAGCCCATCGGCAAACG
TAATCGATGGCGAACCAAATAG

Upstream 100 bases:

>100_bases
AAAAAAGCCGCGGATTCCGCGGCTTTTTTATATAGTTATCTCAATTGTTTAATGTACACTTCTCGCCTCTGAATGATAGG
TAGCGGCGCACATAAGGAAC

Downstream 100 bases:

>100_bases
CAGTGGTAGAGAATGAAGAAACCGAGTTGGATAGAGAAACTGATTGCCCCTAAGGTTGCCTCGCAACAGTTATATGTTGT
GGTGCAGCCAGAGCACCTGT

Product: regulatory protein CsrD

Products: NA

Alternate protein names: Regulator of CsrB and CsrC decay CsrD [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MAQMRYTPTLKLSTRLVAFVTMTVISAMFILFLGGTLSFQRLGQEYLNHYLQGIVEVVDKEMEDPDAAYSMQRWMPKMLQ
ASGIIEMQLTSEAGPIYRFKDTTNTIEASRLHQVALPLKRNPGYVMHFKAVPPYLNYNYSLQAMWSTTLAVLLIVFFLIR
GLKWLKEQLLGSELLEERGRMILAGRVEQHAKGDPREWPFTASEALDRLIEELQDARQERSRFDTFIRSQTFLDQLTGTA
NRVLYDSKLESALLESGAHGGVMMLRVDDLESAREESPKRTLDEFIIEVGECLSNIVQRYPDAILSRYYEDVFALFIPHQ
GSKDIAQVATQAIKLIERINPPEPLPEDNWFHIGVTMYQEGERRGRIIDEMETALKSAQLQGVNAWSRFQKPKQLQEDRG
SVRWRTLFEQVLRPEEILLYRQACYRISANGEREFLHYELFARIQDPQQGILKASRFSSALETVGYEAMLDRAVFSSVVS
FLKRSELVEPLSVNLHVVPFNDKRYARWIRNELMQMPFSLRTALSFEFSEAHLVNHLDYMRPIIRMLAGLGCKIVVGQAG
RTIVSTHYIKDLKVNYLKLHRSLIKKIDQRHENQLFVRSLVGVCGGTQAEVVAVGVETTQEWQTLQMLGVSGVQGRLFDE
EQQMLPPLPPEPLKRPPRLSVKPIGKRNRWRTK

Sequences:

>Translated_673_residues
MAQMRYTPTLKLSTRLVAFVTMTVISAMFILFLGGTLSFQRLGQEYLNHYLQGIVEVVDKEMEDPDAAYSMQRWMPKMLQ
ASGIIEMQLTSEAGPIYRFKDTTNTIEASRLHQVALPLKRNPGYVMHFKAVPPYLNYNYSLQAMWSTTLAVLLIVFFLIR
GLKWLKEQLLGSELLEERGRMILAGRVEQHAKGDPREWPFTASEALDRLIEELQDARQERSRFDTFIRSQTFLDQLTGTA
NRVLYDSKLESALLESGAHGGVMMLRVDDLESAREESPKRTLDEFIIEVGECLSNIVQRYPDAILSRYYEDVFALFIPHQ
GSKDIAQVATQAIKLIERINPPEPLPEDNWFHIGVTMYQEGERRGRIIDEMETALKSAQLQGVNAWSRFQKPKQLQEDRG
SVRWRTLFEQVLRPEEILLYRQACYRISANGEREFLHYELFARIQDPQQGILKASRFSSALETVGYEAMLDRAVFSSVVS
FLKRSELVEPLSVNLHVVPFNDKRYARWIRNELMQMPFSLRTALSFEFSEAHLVNHLDYMRPIIRMLAGLGCKIVVGQAG
RTIVSTHYIKDLKVNYLKLHRSLIKKIDQRHENQLFVRSLVGVCGGTQAEVVAVGVETTQEWQTLQMLGVSGVQGRLFDE
EQQMLPPLPPEPLKRPPRLSVKPIGKRNRWRTK
>Mature_672_residues
AQMRYTPTLKLSTRLVAFVTMTVISAMFILFLGGTLSFQRLGQEYLNHYLQGIVEVVDKEMEDPDAAYSMQRWMPKMLQA
SGIIEMQLTSEAGPIYRFKDTTNTIEASRLHQVALPLKRNPGYVMHFKAVPPYLNYNYSLQAMWSTTLAVLLIVFFLIRG
LKWLKEQLLGSELLEERGRMILAGRVEQHAKGDPREWPFTASEALDRLIEELQDARQERSRFDTFIRSQTFLDQLTGTAN
RVLYDSKLESALLESGAHGGVMMLRVDDLESAREESPKRTLDEFIIEVGECLSNIVQRYPDAILSRYYEDVFALFIPHQG
SKDIAQVATQAIKLIERINPPEPLPEDNWFHIGVTMYQEGERRGRIIDEMETALKSAQLQGVNAWSRFQKPKQLQEDRGS
VRWRTLFEQVLRPEEILLYRQACYRISANGEREFLHYELFARIQDPQQGILKASRFSSALETVGYEAMLDRAVFSSVVSF
LKRSELVEPLSVNLHVVPFNDKRYARWIRNELMQMPFSLRTALSFEFSEAHLVNHLDYMRPIIRMLAGLGCKIVVGQAGR
TIVSTHYIKDLKVNYLKLHRSLIKKIDQRHENQLFVRSLVGVCGGTQAEVVAVGVETTQEWQTLQMLGVSGVQGRLFDEE
QQMLPPLPPEPLKRPPRLSVKPIGKRNRWRTK

Specific function: Serves as a specificity factor required for RNase E- mediated decay of the small global regulatory RNAs CsrB and CsrC, it is probably not a nuclease. Nor does its activity involve c-di- GMP, despite its domain composition. Positively modulates motility ge

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1789650, Length=660, Percent_Identity=33.6363636363636, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI87081921, Length=449, Percent_Identity=21.826280623608, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1787541, Length=430, Percent_Identity=21.1627906976744, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 77505; Mature: 77374

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMRYTPTLKLSTRLVAFVTMTVISAMFILFLGGTLSFQRLGQEYLNHYLQGIVEVVDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EMEDPDAAYSMQRWMPKMLQASGIIEMQLTSEAGPIYRFKDTTNTIEASRLHQVALPLKR
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCC
NPGYVMHFKAVPPYLNYNYSLQAMWSTTLAVLLIVFFLIRGLKWLKEQLLGSELLEERGR
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MILAGRVEQHAKGDPREWPFTASEALDRLIEELQDARQERSRFDTFIRSQTFLDQLTGTA
EEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
NRVLYDSKLESALLESGAHGGVMMLRVDDLESAREESPKRTLDEFIIEVGECLSNIVQRY
HHHEEHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
PDAILSRYYEDVFALFIPHQGSKDIAQVATQAIKLIERINPPEPLPEDNWFHIGVTMYQE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEC
GERRGRIIDEMETALKSAQLQGVNAWSRFQKPKQLQEDRGSVRWRTLFEQVLRPEEILLY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHH
RQACYRISANGEREFLHYELFARIQDPQQGILKASRFSSALETVGYEAMLDRAVFSSVVS
HHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLKRSELVEPLSVNLHVVPFNDKRYARWIRNELMQMPFSLRTALSFEFSEAHLVNHLDYM
HHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
RPIIRMLAGLGCKIVVGQAGRTIVSTHYIKDLKVNYLKLHRSLIKKIDQRHENQLFVRSL
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVCGGTQAEVVAVGVETTQEWQTLQMLGVSGVQGRLFDEEQQMLPPLPPEPLKRPPRLS
HHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
VKPIGKRNRWRTK
CCCCCCCCCCCCC
>Mature Secondary Structure 
AQMRYTPTLKLSTRLVAFVTMTVISAMFILFLGGTLSFQRLGQEYLNHYLQGIVEVVDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
EMEDPDAAYSMQRWMPKMLQASGIIEMQLTSEAGPIYRFKDTTNTIEASRLHQVALPLKR
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCC
NPGYVMHFKAVPPYLNYNYSLQAMWSTTLAVLLIVFFLIRGLKWLKEQLLGSELLEERGR
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MILAGRVEQHAKGDPREWPFTASEALDRLIEELQDARQERSRFDTFIRSQTFLDQLTGTA
EEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
NRVLYDSKLESALLESGAHGGVMMLRVDDLESAREESPKRTLDEFIIEVGECLSNIVQRY
HHHEEHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
PDAILSRYYEDVFALFIPHQGSKDIAQVATQAIKLIERINPPEPLPEDNWFHIGVTMYQE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEC
GERRGRIIDEMETALKSAQLQGVNAWSRFQKPKQLQEDRGSVRWRTLFEQVLRPEEILLY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHH
RQACYRISANGEREFLHYELFARIQDPQQGILKASRFSSALETVGYEAMLDRAVFSSVVS
HHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLKRSELVEPLSVNLHVVPFNDKRYARWIRNELMQMPFSLRTALSFEFSEAHLVNHLDYM
HHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHH
RPIIRMLAGLGCKIVVGQAGRTIVSTHYIKDLKVNYLKLHRSLIKKIDQRHENQLFVRSL
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVCGGTQAEVVAVGVETTQEWQTLQMLGVSGVQGRLFDEEQQMLPPLPPEPLKRPPRLS
HHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
VKPIGKRNRWRTK
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 3049542 [H]