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The map label for this gene is pucG [H]

Identifier: 227080603

GI number: 227080603

Start: 399336

End: 400454

Strand: Direct

Name: pucG [H]

Synonym: VCM66_0377

Alternate gene names: 227080603

Gene position: 399336-400454 (Clockwise)

Preceding gene: 227080601

Following gene: 227080608

Centisome position: 13.81

GC content: 51.12

Gene sequence:

>1119_bases
ATGACAATTCGCAGTTTTATTCCCCCGCATCGGATTTTGATGGGCCCTGGACCTTCGGCTATTTCCCCTCAAGTCCTGCA
AGCGCTCAGCCGCCCAACGGTTGGTCATCTTGATCCACTATTTATCGGTATGATGGATGAGCTGAAACAGCTCTTGAAAT
ATGCTTTCCAAACGGAAAACGAATTTACGATTGCCGTTTCTGCGCCGGGTAGTGCGGGTATGGAAGCCTGTTTTGTTAAC
CTCGTCGAGCCGGGCGATAAAGTGATTGTTTGCCGCAATGGGGTGTTTGGTGAGCGGATGCGCGATAACGCTGTGCGTTG
TGGGGCCGAAGTTGTGGTGGTTGATGATGAATGGGGTTGCCCAGTCAGCGTTGAAAAAGTGCAAGCCACGATAGATGAGC
ATCCGGATGCGAAAATACTGGCTTTTGTACAAGCAGAAACGTCAACCGGAGCCTTAAGTGATGCGCAAGCGTTAGGTGCT
CTAGCGAAGCAGCATGGTTTGTTGACGATTGTTGATGCGGTGACCTCGCTCGGTGGCGTTCCCCTGCTGGTGGATGAGTG
GCAACTGGATGCGGTGTATTCTGGCAGCCAAAAATGCCTCTCTTGTGTGCCGGGATTGGCTCCGTTGACGTTTTCACCTC
GTGCCATTGAGAAGATTCAATCGCGAAAAACACCGATTCAAAGCTGGTTCCTCGATCAAAGCTTGGTATTAGGTTATTGG
AGTGGCGCGGGCAAACGTAGTTATCACCATACCGCTCCGGTTAATAGTTTGTACGCGCTGCACGAAGCGTTACTGCTTTT
GCAACAAGAAGGTTTAGAGCACGCGTGGCAGCGCCATCGTCTGATGCATGAGAAGCTTAAACAAGGGTTAGAGCAACTCG
GTTTTCAATTTGTGGTCGCAGAGCCGTATCGTTTACCGCAGTTGAACGCGATTTATGTGCCGGAAGGTATTGAGGAAGCC
AAAGTACGCAGCCATCTACTCAATGAATACAATTTGGAAATTGGTGCTGGGCTCGGGGCATTAGCCGGCAAAGCGTGGCG
CATTGGGGTTATGGGCTATGGCGCGCGTTCAGAAAATATCGCGCTCTGTTTGCGAGCGCTCGAAGAGTCGCTGCGTTAA

Upstream 100 bases:

>100_bases
TTAGTGTGACAAGGATTAACACTCTGGACATAGGTCGAATTGTTGCTGCTACTGAATTGCATTAGGCTAGTGGTTCTGGT
CGCCACAACAAGGAGTCGTT

Downstream 100 bases:

>100_bases
TACCATACGCAAGAGAAAAGCTGTATGAAAGAGAAATAAGGGTGGCTTAAAAGCCACCCTTATTGTTTCTCGCAGAGCTT
AGGGTGTCGCGGCTGAGGGA

Product: aminotransferase, class V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MTIRSFIPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQTENEFTIAVSAPGSAGMEACFVN
LVEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDEWGCPVSVEKVQATIDEHPDAKILAFVQAETSTGALSDAQALGA
LAKQHGLLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKTPIQSWFLDQSLVLGYW
SGAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHRLMHEKLKQGLEQLGFQFVVAEPYRLPQLNAIYVPEGIEEA
KVRSHLLNEYNLEIGAGLGALAGKAWRIGVMGYGARSENIALCLRALEESLR

Sequences:

>Translated_372_residues
MTIRSFIPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQTENEFTIAVSAPGSAGMEACFVN
LVEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDEWGCPVSVEKVQATIDEHPDAKILAFVQAETSTGALSDAQALGA
LAKQHGLLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKTPIQSWFLDQSLVLGYW
SGAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHRLMHEKLKQGLEQLGFQFVVAEPYRLPQLNAIYVPEGIEEA
KVRSHLLNEYNLEIGAGLGALAGKAWRIGVMGYGARSENIALCLRALEESLR
>Mature_371_residues
TIRSFIPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQTENEFTIAVSAPGSAGMEACFVNL
VEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDEWGCPVSVEKVQATIDEHPDAKILAFVQAETSTGALSDAQALGAL
AKQHGLLTIVDAVTSLGGVPLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKTPIQSWFLDQSLVLGYWS
GAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHRLMHEKLKQGLEQLGFQFVVAEPYRLPQLNAIYVPEGIEEAK
VRSHLLNEYNLEIGAGLGALAGKAWRIGVMGYGARSENIALCLRALEESLR

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=36.8852459016393, Blast_Score=254, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17536281, Length=373, Percent_Identity=39.9463806970509, Blast_Score=274, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6321079, Length=375, Percent_Identity=28.2666666666667, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17530823, Length=372, Percent_Identity=40.0537634408602, Blast_Score=271, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 40624; Mature: 40493

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRSFIPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQTEN
CCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
EFTIAVSAPGSAGMEACFVNLVEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDEWGC
CEEEEEECCCCCCHHHHHHEEECCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCC
PVSVEKVQATIDEHPDAKILAFVQAETSTGALSDAQALGALAKQHGLLTIVDAVTSLGGV
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKTPIQSWFLDQSLVLGYW
EEEEECCEECHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCHHEEEEE
SGAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHRLMHEKLKQGLEQLGFQFVVA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
EPYRLPQLNAIYVPEGIEEAKVRSHLLNEYNLEIGAGLGALAGKAWRIGVMGYGARSENI
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEECCCHHHHCCCEEEEEEEECCCCCCHH
ALCLRALEESLR
HHHHHHHHHHCC
>Mature Secondary Structure 
TIRSFIPPHRILMGPGPSAISPQVLQALSRPTVGHLDPLFIGMMDELKQLLKYAFQTEN
CCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
EFTIAVSAPGSAGMEACFVNLVEPGDKVIVCRNGVFGERMRDNAVRCGAEVVVVDDEWGC
CEEEEEECCCCCCHHHHHHEEECCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCC
PVSVEKVQATIDEHPDAKILAFVQAETSTGALSDAQALGALAKQHGLLTIVDAVTSLGGV
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PLLVDEWQLDAVYSGSQKCLSCVPGLAPLTFSPRAIEKIQSRKTPIQSWFLDQSLVLGYW
EEEEECCEECHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCHHEEEEE
SGAGKRSYHHTAPVNSLYALHEALLLLQQEGLEHAWQRHRLMHEKLKQGLEQLGFQFVVA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
EPYRLPQLNAIYVPEGIEEAKVRSHLLNEYNLEIGAGLGALAGKAWRIGVMGYGARSENI
CCCCCCCCCEEECCCCHHHHHHHHHHHHHCCEEECCCHHHHCCCEEEEEEEECCCCCCHH
ALCLRALEESLR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]