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The map label for this gene is fre [H]

Identifier: 227080525

GI number: 227080525

Start: 307577

End: 308287

Strand: Direct

Name: fre [H]

Synonym: VCM66_0296

Alternate gene names: 227080525

Gene position: 307577-308287 (Clockwise)

Preceding gene: 227080524

Following gene: 227080526

Centisome position: 10.63

GC content: 48.8

Gene sequence:

>711_bases
ATGATAATTCAATGCCAAGTTAAGTCTGTGCAGCCGCTGGCGACGCATACTTACCAAATCCTATTACAACCCGAGCACGC
GGTGGCTTACCAAGCCGGGCAGTATTTAATGGTGGTGATGGGCGAAAAAGATAAGCGACCATTTTCACTTGCCAGTAGTC
CTTGCCGTAGCAATGGCGAGCTCGAACTGCATATTGGTGCGGCGGATCACAGCGCCTTTGCCCACCAAGTCGTGGAAAAA
TTTCAGCAAGCCCATCTTAATCAGACATGGGTTGAAGTAGATGTGCCACATGGCAATGCCGCATTACAAGAGAGTGAGCG
ACCTCTACTCTTAATCGCTGGCGGGACCGGCTTTAGCTATGTGCGTTCCATCTTAGATCATTGCCTCTCGCAAGGTAAAA
CTCAGCCTATCTACCTGTATTGGGGCGCGCGTGATGCAGCTCAGCTGTACGCACTGAATGAGTTGCAAGAGCTTGCCAAG
CAACATGCTCATTTGCAGGTTGTTCCAGTGGTCGAGCAAGCACAAGATGACTGGGCTGGAAAAGTTGGCAATGTACTGCA
AGCGATTAACAACGACTTTGCAAGTTTAGAAGCATTTGACATCTATATAGCAGGTCGCTTTGAGATGGCTGGAGCCGCAC
GTGAGCAATTTACTCAAAACAAACAAGCTCGACGCGATCGAATGTTTGCCGATGCTTACGCCTTTATTTAA

Upstream 100 bases:

>100_bases
GACCGAACAAGAAAAAGCGACAGGGTGGATATTTGCCTGCCAAGCCTGCGCAATTACTGATTTAGTGCTGACTTTTGAAG
AGTAAGCCAGAGGAAGTTCA

Downstream 100 bases:

>100_bases
CGCGCAGCTATAAACAAGATACAAGACAAAGCCCAACGCTAAATCGTTGGGCTTTTTTATGACTTTCTCTTTATAGATAG
ACTAGAGCAAAAGCTTAAAA

Product: FMN reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIIQCQVKSVQPLATHTYQILLQPEHAVAYQAGQYLMVVMGEKDKRPFSLASSPCRSNGELELHIGAADHSAFAHQVVEK
FQQAHLNQTWVEVDVPHGNAALQESERPLLLIAGGTGFSYVRSILDHCLSQGKTQPIYLYWGARDAAQLYALNELQELAK
QHAHLQVVPVVEQAQDDWAGKVGNVLQAINNDFASLEAFDIYIAGRFEMAGAAREQFTQNKQARRDRMFADAYAFI

Sequences:

>Translated_236_residues
MIIQCQVKSVQPLATHTYQILLQPEHAVAYQAGQYLMVVMGEKDKRPFSLASSPCRSNGELELHIGAADHSAFAHQVVEK
FQQAHLNQTWVEVDVPHGNAALQESERPLLLIAGGTGFSYVRSILDHCLSQGKTQPIYLYWGARDAAQLYALNELQELAK
QHAHLQVVPVVEQAQDDWAGKVGNVLQAINNDFASLEAFDIYIAGRFEMAGAAREQFTQNKQARRDRMFADAYAFI
>Mature_236_residues
MIIQCQVKSVQPLATHTYQILLQPEHAVAYQAGQYLMVVMGEKDKRPFSLASSPCRSNGELELHIGAADHSAFAHQVVEK
FQQAHLNQTWVEVDVPHGNAALQESERPLLLIAGGTGFSYVRSILDHCLSQGKTQPIYLYWGARDAAQLYALNELQELAK
QHAHLQVVPVVEQAQDDWAGKVGNVLQAINNDFASLEAFDIYIAGRFEMAGAAREQFTQNKQARRDRMFADAYAFI

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Preferably uses riboflavin as an electron acceptor when NADPH is used as an electron donor [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=235, Percent_Identity=48.5106382978723, Blast_Score=231, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 26337; Mature: 26337

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQCQVKSVQPLATHTYQILLQPEHAVAYQAGQYLMVVMGEKDKRPFSLASSPCRSNGE
CEEEEECCCCCCHHHCEEEEEECCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCE
LELHIGAADHSAFAHQVVEKFQQAHLNQTWVEVDVPHGNAALQESERPLLLIAGGTGFSY
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCHHH
VRSILDHCLSQGKTQPIYLYWGARDAAQLYALNELQELAKQHAHLQVVPVVEQAQDDWAG
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHH
KVGNVLQAINNDFASLEAFDIYIAGRFEMAGAAREQFTQNKQARRDRMFADAYAFI
HHHHHHHHHCCCCCCEEEEEEEEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIQCQVKSVQPLATHTYQILLQPEHAVAYQAGQYLMVVMGEKDKRPFSLASSPCRSNGE
CEEEEECCCCCCHHHCEEEEEECCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCE
LELHIGAADHSAFAHQVVEKFQQAHLNQTWVEVDVPHGNAALQESERPLLLIAGGTGFSY
EEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCEEEEECCCCHHH
VRSILDHCLSQGKTQPIYLYWGARDAAQLYALNELQELAKQHAHLQVVPVVEQAQDDWAG
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCHHHHH
KVGNVLQAINNDFASLEAFDIYIAGRFEMAGAAREQFTQNKQARRDRMFADAYAFI
HHHHHHHHHCCCCCCEEEEEEEEEECEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8167139; 8206831; 6981058 [H]