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The map label for this gene is glpE

Identifier: 227080336

GI number: 227080336

Start: 98751

End: 99071

Strand: Reverse

Name: glpE

Synonym: VCM66_0100

Alternate gene names: 227080336

Gene position: 99071-98751 (Counterclockwise)

Preceding gene: 227080337

Following gene: 227080335

Centisome position: 3.43

GC content: 46.11

Gene sequence:

>321_bases
ATGGACCATTTTTTACACATCGACGTTAATGCAGCACAAGCCATGATGGAGCAGAAACAGGCTCATTTAGTCGATATTCG
CGACCCACAATCTTTTCAACTCGCTCATGCTAAAAACGCTTACCACCTGACAAACCAGTCTATGGTGCAATTCATGGAAC
AAGCTGAGTTTGATCAGCCGGTTTTAGTCATGTGTTATCACGGCATAAGTAGCCAAGGCGCAGCCCAATATCTGGTTAAC
CAAGGATTTGAAGAGGTGTATAGTGTCGATGGCGGCTTTGAAGCCTGGCATCGTGCCAATCTCCCCATCGAAGCGAGTTA
A

Upstream 100 bases:

>100_bases
TTTTAATCACAAGTGGATAACCCTACTCTTGTCCAGTACTTGTCTAAACTTGAATAGGTTACAATTAGCCCGATTCGGCA
TGCCAAATGAGAGCAAACCT

Downstream 100 bases:

>100_bases
GGACTAATGCATTTACTGACCACTTTTAACAATCCGCGTGCGGCACAAGCTTTTATCGATTACATGGCCGCCCACCACAT
TGAAATTCAGATGATGCCGG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MDHFLHIDVNAAQAMMEQKQAHLVDIRDPQSFQLAHAKNAYHLTNQSMVQFMEQAEFDQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRANLPIEAS

Sequences:

>Translated_106_residues
MDHFLHIDVNAAQAMMEQKQAHLVDIRDPQSFQLAHAKNAYHLTNQSMVQFMEQAEFDQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRANLPIEAS
>Mature_106_residues
MDHFLHIDVNAAQAMMEQKQAHLVDIRDPQSFQLAHAKNAYHLTNQSMVQFMEQAEFDQPVLVMCYHGISSQGAAQYLVN
QGFEEVYSVDGGFEAWHRANLPIEAS

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1789831, Length=106, Percent_Identity=56.6037735849057, Blast_Score=127, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPE_VIBC3 (A5F4G9)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4G9
- SMR:   A5F4G9
- STRING:   A5F4G9
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2416
- eggNOG:   COG0607
- HOGENOM:   HBG743107
- OMA:   FSAWHEA
- ProtClustDB:   PRK00162
- BioCyc:   VCHO345073:VC0395_A2416-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01009
- InterPro:   IPR001763
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 12024; Mature: 12024

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHFLHIDVNAAQAMMEQKQAHLVDIRDPQSFQLAHAKNAYHLTNQSMVQFMEQAEFDQP
CCCEEEEECHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEECHHHHHHHHHHCCCCCC
VLVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEAWHRANLPIEAS
EEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCEECC
>Mature Secondary Structure
MDHFLHIDVNAAQAMMEQKQAHLVDIRDPQSFQLAHAKNAYHLTNQSMVQFMEQAEFDQP
CCCEEEEECHHHHHHHHHHHHHEEECCCCCCEEEECCCCCEEECHHHHHHHHHHCCCCCC
VLVMCYHGISSQGAAQYLVNQGFEEVYSVDGGFEAWHRANLPIEAS
EEEEEECCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA