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The map label for this gene is ilvG [H]

Identifier: 227080268

GI number: 227080268

Start: 27889

End: 29535

Strand: Reverse

Name: ilvG [H]

Synonym: VCM66_0031

Alternate gene names: 227080268

Gene position: 29535-27889 (Counterclockwise)

Preceding gene: 227080271

Following gene: 227080267

Centisome position: 1.02

GC content: 55.31

Gene sequence:

>1647_bases
ATGACCGGAGCACAGTTAGTCGTTGCCGCATTGAAACAACAGGGCATCAAAACCGTTTTTGGTTACCCTGGCGGCGCTAT
CATGCCGATTTATGATGCGCTGTATGACGGAGGTGTTGAGCACATCCTCTGCCGCCATGAACAAGGTGCGGCGATGGCCG
CGATTGGTATGGCGCGCTCAACGCAGAAAGTGGCGGTGTGCATGGCCACATCTGGCCCCGGCGCCACCAACTTGGTGACA
GGTTTGGCGGATGCCTTTCTTGATTCGGTTCCGCTGGTCGCCATCACCGGCCAAGTGGCCAGCTCACACATCGGCACCGA
TGCGTTTCAAGAGATGGATGTGATCGGCATGTCGCTCTCTTGTACCAAGCACAGCTACTTAGTCACCGACATCAACGAGC
TAGCACCCACGTTAGCCGAAGCGTTTGAGGTCGCGCAGACCGGTCGTCCCGGCCCCGTGCTGGTGGACATAGCCAAAGAC
GTACAGCTCGGCAAAGCGCCGGTCTCCGCTTTGCCAAGCTTTACTCCACCCGCCATGCCACACGTGGATGCAACACATCT
GGCGAATGCGCAAGCCTTACTTGCACAAAGCAAACGCCCTGTATTGTATGTGGGTGGAGGTGTACAACTGGCGAATGCCA
CAGACACAGTGCGTGAGTTTTTACGCCTCAATCCTATGCCTTCAGTGAGCACACTCAAAGGCTTGGGCACCATCGAGCGT
CACGACCCACACTATCTTGGCATGCTCGGCATGCACGGCACCAAAGCGGCAAACTTAATCGTACAAGAGTGTGATTTGCT
GATCGCGGTTGGTGCGCGTTTTGATGACCGCGTGACAGGCAAGCTCGATACCTTTGCGCCTCATGCCAAAGTGATTCATG
TCGATATTGATGCCGCCGAGTTCAACAAACTGCGTCATGCCCATGCTGCACTGCGCGGTGATATCAACCTCATCCTGCCG
CAGTTGGAACTGAGCCACGACATCTCCCCTTGGGTGCATCATAGTGAGAGCCTGCGTAGCGGGTTCAAATGGCGCTACGA
TCATCCGGGCGACAACATCTTCGCACCACTGCTGCTCAAGCAACTGTCGGACATGATGCCGGACTCTTCGATTGTCTCGA
CCGATGTCGGCCAACACCAAATGTGGGCCGCGCAGCACATTCAACCGCGCGCACCACAAAACTTCATCTCGTCCGCAGGC
CTTGGCACCATGGGCTTTGGTTTACCGGCGGCAATGGGTGCTGCGGTTGCGCGTCCTGACGATCAATCGATCCTGATCAG
TGGTGATGGTTCTTTCATGATGAACGTGCAAGAACTTGGCACACTCAAGCGTCGCCAGATCCCCGTCAAAATGGTGCTAC
TGGATAACCAACGTTTGGGCATGGTACGCCAATGGCAATCACTGTTCTTTGATGGTCGCCACAGCGAAACCATTTTGGAT
GACAACCCAGATTTCGTGATGCTGGCCAAAGCCTTCAACATCCCCGGCAAAACCATCAGCCGCAAAGAAGAAGTCGAACC
TGCGCTGCGTGAAATGCTGGCCAGCAAAACCGCTTATCTGCTGCACGTGCTGATTGATGAAGAAGAGAACGTGTGGCCAC
TGGTGCCACCGGGCGCATCCAACACTGACATGCTTGAGAACACCTAA

Upstream 100 bases:

>100_bases
AATACCCCCAGAATACGCAGGAAGAATGGGAGCGGCACATGAAGTGCAAACACAACACACAGGAAGGCAGCGACACGTAT
TGTTGCCATAAGGAGGCACG

Downstream 100 bases:

>100_bases
GGAGAGGCCACATGCAACGTTATCTACTCGATATTAAAGCGGACGATAAACCGGTGTTATTAGAGCGCGTATTGCGCGTA
ACGCGTCATCGTGGATTTGT

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTGAQLVVAALKQQGIKTVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARSTQKVAVCMATSGPGATNLVT
GLADAFLDSVPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPTLAEAFEVAQTGRPGPVLVDIAKD
VQLGKAPVSALPSFTPPAMPHVDATHLANAQALLAQSKRPVLYVGGGVQLANATDTVREFLRLNPMPSVSTLKGLGTIER
HDPHYLGMLGMHGTKAANLIVQECDLLIAVGARFDDRVTGKLDTFAPHAKVIHVDIDAAEFNKLRHAHAALRGDINLILP
QLELSHDISPWVHHSESLRSGFKWRYDHPGDNIFAPLLLKQLSDMMPDSSIVSTDVGQHQMWAAQHIQPRAPQNFISSAG
LGTMGFGLPAAMGAAVARPDDQSILISGDGSFMMNVQELGTLKRRQIPVKMVLLDNQRLGMVRQWQSLFFDGRHSETILD
DNPDFVMLAKAFNIPGKTISRKEEVEPALREMLASKTAYLLHVLIDEEENVWPLVPPGASNTDMLENT

Sequences:

>Translated_548_residues
MTGAQLVVAALKQQGIKTVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARSTQKVAVCMATSGPGATNLVT
GLADAFLDSVPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPTLAEAFEVAQTGRPGPVLVDIAKD
VQLGKAPVSALPSFTPPAMPHVDATHLANAQALLAQSKRPVLYVGGGVQLANATDTVREFLRLNPMPSVSTLKGLGTIER
HDPHYLGMLGMHGTKAANLIVQECDLLIAVGARFDDRVTGKLDTFAPHAKVIHVDIDAAEFNKLRHAHAALRGDINLILP
QLELSHDISPWVHHSESLRSGFKWRYDHPGDNIFAPLLLKQLSDMMPDSSIVSTDVGQHQMWAAQHIQPRAPQNFISSAG
LGTMGFGLPAAMGAAVARPDDQSILISGDGSFMMNVQELGTLKRRQIPVKMVLLDNQRLGMVRQWQSLFFDGRHSETILD
DNPDFVMLAKAFNIPGKTISRKEEVEPALREMLASKTAYLLHVLIDEEENVWPLVPPGASNTDMLENT
>Mature_547_residues
TGAQLVVAALKQQGIKTVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARSTQKVAVCMATSGPGATNLVTG
LADAFLDSVPLVAITGQVASSHIGTDAFQEMDVIGMSLSCTKHSYLVTDINELAPTLAEAFEVAQTGRPGPVLVDIAKDV
QLGKAPVSALPSFTPPAMPHVDATHLANAQALLAQSKRPVLYVGGGVQLANATDTVREFLRLNPMPSVSTLKGLGTIERH
DPHYLGMLGMHGTKAANLIVQECDLLIAVGARFDDRVTGKLDTFAPHAKVIHVDIDAAEFNKLRHAHAALRGDINLILPQ
LELSHDISPWVHHSESLRSGFKWRYDHPGDNIFAPLLLKQLSDMMPDSSIVSTDVGQHQMWAAQHIQPRAPQNFISSAGL
GTMGFGLPAAMGAAVARPDDQSILISGDGSFMMNVQELGTLKRRQIPVKMVLLDNQRLGMVRQWQSLFFDGRHSETILDD
NPDFVMLAKAFNIPGKTISRKEEVEPALREMLASKTAYLLHVLIDEEENVWPLVPPGASNTDMLENT

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=24.5519713261649, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=504, Percent_Identity=25.5952380952381, Blast_Score=142, Evalue=9e-34,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=46.5454545454545, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=41.0211267605634, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=27.9926335174954, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=29.1208791208791, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788716, Length=548, Percent_Identity=25.5474452554745, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=482, Percent_Identity=27.8008298755187, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=482, Percent_Identity=27.8008298755187, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=594, Percent_Identity=24.2424242424242, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=43.0823117338004, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320816, Length=479, Percent_Identity=22.7557411273486, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=495, Percent_Identity=23.4343434343434, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=489, Percent_Identity=21.2678936605317, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922626, Length=541, Percent_Identity=25.3234750462107, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59075; Mature: 58943

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAQLVVAALKQQGIKTVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARS
CCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHEEEECCCCCEEHHHHHHCC
TQKVAVCMATSGPGATNLVTGLADAFLDSVPLVAITGQVASSHIGTDAFQEMDVIGMSLS
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCHHHHHHHHHCEEEE
CTKHSYLVTDINELAPTLAEAFEVAQTGRPGPVLVDIAKDVQLGKAPVSALPSFTPPAMP
CCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCC
HVDATHLANAQALLAQSKRPVLYVGGGVQLANATDTVREFLRLNPMPSVSTLKGLGTIER
CCCHHHHCCHHHHHHCCCCCEEEECCCEEECCHHHHHHHHHHCCCCCCHHHHHCCCCCCC
HDPHYLGMLGMHGTKAANLIVQECDLLIAVGARFDDRVTGKLDTFAPHAKVIHVDIDAAE
CCCCEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHH
FNKLRHAHAALRGDINLILPQLELSHDISPWVHHSESLRSGFKWRYDHPGDNIFAPLLLK
HHHHHHHHHHHHCCCEEEEECCHHCCCCCHHHHCCHHHHCCCCEECCCCCCCHHHHHHHH
QLSDMMPDSSIVSTDVGQHQMWAAQHIQPRAPQNFISSAGLGTMGFGLPAAMGAAVARPD
HHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCC
DQSILISGDGSFMMNVQELGTLKRRQIPVKMVLLDNQRLGMVRQWQSLFFDGRHSETILD
CCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEC
DNPDFVMLAKAFNIPGKTISRKEEVEPALREMLASKTAYLLHVLIDEEENVWPLVPPGAS
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCC
NTDMLENT
CCCCCCCC
>Mature Secondary Structure 
TGAQLVVAALKQQGIKTVFGYPGGAIMPIYDALYDGGVEHILCRHEQGAAMAAIGMARS
CCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHEEEECCCCCEEHHHHHHCC
TQKVAVCMATSGPGATNLVTGLADAFLDSVPLVAITGQVASSHIGTDAFQEMDVIGMSLS
CCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCHHHHHHHHHCEEEE
CTKHSYLVTDINELAPTLAEAFEVAQTGRPGPVLVDIAKDVQLGKAPVSALPSFTPPAMP
CCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCC
HVDATHLANAQALLAQSKRPVLYVGGGVQLANATDTVREFLRLNPMPSVSTLKGLGTIER
CCCHHHHCCHHHHHHCCCCCEEEECCCEEECCHHHHHHHHHHCCCCCCHHHHHCCCCCCC
HDPHYLGMLGMHGTKAANLIVQECDLLIAVGARFDDRVTGKLDTFAPHAKVIHVDIDAAE
CCCCEEEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCHHH
FNKLRHAHAALRGDINLILPQLELSHDISPWVHHSESLRSGFKWRYDHPGDNIFAPLLLK
HHHHHHHHHHHHCCCEEEEECCHHCCCCCHHHHCCHHHHCCCCEECCCCCCCHHHHHHHH
QLSDMMPDSSIVSTDVGQHQMWAAQHIQPRAPQNFISSAGLGTMGFGLPAAMGAAVARPD
HHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCC
DQSILISGDGSFMMNVQELGTLKRRQIPVKMVLLDNQRLGMVRQWQSLFFDGRHSETILD
CCEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEC
DNPDFVMLAKAFNIPGKTISRKEEVEPALREMLASKTAYLLHVLIDEEENVWPLVPPGAS
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCC
NTDMLENT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]