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The map label for this gene is trmE

Identifier: 226951095

GI number: 226951095

Start: 4151394

End: 4152779

Strand: Reverse

Name: trmE

Synonym: CLM_4148

Alternate gene names: 226951095

Gene position: 4152779-4151394 (Counterclockwise)

Preceding gene: 226951096

Following gene: 226951094

Centisome position: 99.94

GC content: 27.06

Gene sequence:

>1386_bases
ATGAAAGAATTTGATACTATAGCTGCTGTAGCAACACCGGTAGGAGAAGGTGGTATATCTATTATAAGAATATCAGGAGA
TAAGTCATTAGATATAGTAAGTAGCATATTTAAAGGTAAAAATGATAGAACATTAGATGATATAAAACCTTATTCTATGA
GATATGGCTTTATAATTGAAAAAGAAAGTAAAGAAATGATAGATGAAGTTCTAGTAAGTTATATGAAAGGCCCTAGAAGC
TTCACTGCTGAGGATACTTTAGAAATTAATTGCCATGGGGGTGTAATACCAACAAAAAAAATATTAAAAGAATTAATTAA
ATCTGGAGCAAGGCTTGCAGAGCCAGGGGAATTTACTAAAAGAGCTTTTTTAAATGGAAGAATAGATTTAAGTCAAGCAG
AGGCTGTTATAGATATAATAAGATCTAAAACAGACCTTTCTATGAAGTCTGCATTAAAGCAGGCTGAAGGAACTCTTTCA
AAGGAAATTAATTCCATTAGAAATAGAATGATTAAAATTATAGCACATATAGAAGCTACTGTAGATTATCCTGAAGATGA
TTTAGAGGAAATAACAGGCCAAAAAATTAAAGTAGATTTAAAAGAGATAATAAATAAAATAGATAATTTGATAAGTGCAT
CAGAAGAAGGTAAAATATTAAGAGAAGGGTTAAATACTGTAATAGTTGGAAAACCTAATGTAGGAAAATCTTCTTTGTTA
AATGCATTAATCAATGAGAATAAAGCTATAGTCACAGAAATACCAGGAACTACAAGAGATGTTATAGAAGAATATATAAA
TATTGATGGAATACCTATAAAAATAGTAGATACAGCAGGAATAAGAGAAACAGAAGATGTAGTAGAAAAAATTGGTGTAG
AAAAATCAAAAGAGAAGATCGATGAAGCTGATTTAGTTATATTCATGTTAGATTTAAGCAGAAAAATAGATGAAGAAGAC
ATAGAGATAATGGATTTTATAAAAAATAAAAAGTATATAGTTTTATTAAATAAATTAGATTTAAATAAAGATTTAAATGA
AGAAAATCATTTTATAAAAGAATTAGATTCTAAATATATAATAAAAACATCAGTTAAAAACAATTCAGGATTGAATGAAT
TAAAAGAATGTATAAAAAATCTTTTCTTTAGTGGAGAAATAAAATCTGATGAACTTATAGTTACAAATGCTAGGCATCAG
GAAGCTTTAATAAGATCTAGAGAATCTTGTATTCAAGCTATAGAAACTTTATCAGATGAAATATCAATAGATTTAGCATC
TATAGATATTAGAAATGCGTGGAAATATCTTGGAGAAATTACTGGTGATACTTTAGATGAGAACATTATAGATAAAATAT
TTTCTGAATTTTGTTTAGGAAAGTAG

Upstream 100 bases:

>100_bases
GTGAAGGTGAAGAGCCTTTTAGAAAAGTTGTAATAAATTTAAGGAAAGCCTAAAGGCTTTCTTTTTATATTATTTGTAAT
TTGTAAAAAGAGGTGATTTT

Downstream 100 bases:

>100_bases
GTAATTATATGAAATATTTAGCTGGAGATTTTGATGTAGTAGTTATTGGCGCAGGTCATGCGGGATGCGAGGCTGCATTA
GCTTCTGCAAGAATGGGATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKEFDTIAAVATPVGEGGISIIRISGDKSLDIVSSIFKGKNDRTLDDIKPYSMRYGFIIEKESKEMIDEVLVSYMKGPRS
FTAEDTLEINCHGGVIPTKKILKELIKSGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRSKTDLSMKSALKQAEGTLS
KEINSIRNRMIKIIAHIEATVDYPEDDLEEITGQKIKVDLKEIINKIDNLISASEEGKILREGLNTVIVGKPNVGKSSLL
NALINENKAIVTEIPGTTRDVIEEYINIDGIPIKIVDTAGIRETEDVVEKIGVEKSKEKIDEADLVIFMLDLSRKIDEED
IEIMDFIKNKKYIVLLNKLDLNKDLNEENHFIKELDSKYIIKTSVKNNSGLNELKECIKNLFFSGEIKSDELIVTNARHQ
EALIRSRESCIQAIETLSDEISIDLASIDIRNAWKYLGEITGDTLDENIIDKIFSEFCLGK

Sequences:

>Translated_461_residues
MKEFDTIAAVATPVGEGGISIIRISGDKSLDIVSSIFKGKNDRTLDDIKPYSMRYGFIIEKESKEMIDEVLVSYMKGPRS
FTAEDTLEINCHGGVIPTKKILKELIKSGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRSKTDLSMKSALKQAEGTLS
KEINSIRNRMIKIIAHIEATVDYPEDDLEEITGQKIKVDLKEIINKIDNLISASEEGKILREGLNTVIVGKPNVGKSSLL
NALINENKAIVTEIPGTTRDVIEEYINIDGIPIKIVDTAGIRETEDVVEKIGVEKSKEKIDEADLVIFMLDLSRKIDEED
IEIMDFIKNKKYIVLLNKLDLNKDLNEENHFIKELDSKYIIKTSVKNNSGLNELKECIKNLFFSGEIKSDELIVTNARHQ
EALIRSRESCIQAIETLSDEISIDLASIDIRNAWKYLGEITGDTLDENIIDKIFSEFCLGK
>Mature_461_residues
MKEFDTIAAVATPVGEGGISIIRISGDKSLDIVSSIFKGKNDRTLDDIKPYSMRYGFIIEKESKEMIDEVLVSYMKGPRS
FTAEDTLEINCHGGVIPTKKILKELIKSGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIIRSKTDLSMKSALKQAEGTLS
KEINSIRNRMIKIIAHIEATVDYPEDDLEEITGQKIKVDLKEIINKIDNLISASEEGKILREGLNTVIVGKPNVGKSSLL
NALINENKAIVTEIPGTTRDVIEEYINIDGIPIKIVDTAGIRETEDVVEKIGVEKSKEKIDEADLVIFMLDLSRKIDEED
IEIMDFIKNKKYIVLLNKLDLNKDLNEENHFIKELDSKYIIKTSVKNNSGLNELKECIKNLFFSGEIKSDELIVTNARHQ
EALIRSRESCIQAIETLSDEISIDLASIDIRNAWKYLGEITGDTLDENIIDKIFSEFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=474, Percent_Identity=32.2784810126582, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI193082988, Length=506, Percent_Identity=30.6324110671937, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=31.9654427645788, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=34.698275862069, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI87082120, Length=200, Percent_Identity=31, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=470, Percent_Identity=30.8510638297872, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=33.739837398374, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI45550813, Length=488, Percent_Identity=32.5819672131148, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CLOB1 (A7FPM0)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385957.1
- ProteinModelPortal:   A7FPM0
- SMR:   A7FPM0
- STRING:   A7FPM0
- GeneID:   5395054
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3736
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   CBOT441770:CLB_3736-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51669; Mature: 51669

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 88-88 BINDING 127-127 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFDTIAAVATPVGEGGISIIRISGDKSLDIVSSIFKGKNDRTLDDIKPYSMRYGFIIE
CCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCEEEEEEEE
KESKEMIDEVLVSYMKGPRSFTAEDTLEINCHGGVIPTKKILKELIKSGARLAEPGEFTK
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLSQAEAVIDIIRSKTDLSMKSALKQAEGTLSKEINSIRNRMIKIIAHIEAT
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDYPEDDLEEITGQKIKVDLKEIINKIDNLISASEEGKILREGLNTVIVGKPNVGKSSLL
CCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCHHHHH
NALINENKAIVTEIPGTTRDVIEEYINIDGIPIKIVDTAGIRETEDVVEKIGVEKSKEKI
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHC
DEADLVIFMLDLSRKIDEEDIEIMDFIKNKKYIVLLNKLDLNKDLNEENHFIKELDSKYI
CHHCEEEEEEEHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEE
IKTSVKNNSGLNELKECIKNLFFSGEIKSDELIVTNARHQEALIRSRESCIQAIETLSDE
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ISIDLASIDIRNAWKYLGEITGDTLDENIIDKIFSEFCLGK
HEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEFDTIAAVATPVGEGGISIIRISGDKSLDIVSSIFKGKNDRTLDDIKPYSMRYGFIIE
CCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCEEEEEEEE
KESKEMIDEVLVSYMKGPRSFTAEDTLEINCHGGVIPTKKILKELIKSGARLAEPGEFTK
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRIDLSQAEAVIDIIRSKTDLSMKSALKQAEGTLSKEINSIRNRMIKIIAHIEAT
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDYPEDDLEEITGQKIKVDLKEIINKIDNLISASEEGKILREGLNTVIVGKPNVGKSSLL
CCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCHHHHH
NALINENKAIVTEIPGTTRDVIEEYINIDGIPIKIVDTAGIRETEDVVEKIGVEKSKEKI
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHC
DEADLVIFMLDLSRKIDEEDIEIMDFIKNKKYIVLLNKLDLNKDLNEENHFIKELDSKYI
CHHCEEEEEEEHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEE
IKTSVKNNSGLNELKECIKNLFFSGEIKSDELIVTNARHQEALIRSRESCIQAIETLSDE
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
ISIDLASIDIRNAWKYLGEITGDTLDENIIDKIFSEFCLGK
HEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA