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The map label for this gene is lysS [H]

Identifier: 226950975

GI number: 226950975

Start: 4028657

End: 4030171

Strand: Reverse

Name: lysS [H]

Synonym: CLM_4004

Alternate gene names: 226950975

Gene position: 4030171-4028657 (Counterclockwise)

Preceding gene: 226950976

Following gene: 226950974

Centisome position: 96.99

GC content: 29.7

Gene sequence:

>1515_bases
GTGTCAAAGGAAGATAATGTGATAAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGAAAGATTCCAAAAGTTAAA
GGAACTTCAATCTAATGGCAAAGATCCATTTGATGTTTATAAGGTTGAAAGAACCCATACTTCAAAAGAAGTAAAGGATA
ATTATGAAAATCTAGAAGGAAAAACTGTCACAGTGGCAGGAAGACTTATGTCAAAAAGAGTTCATGGAAAAGCTGGATTT
TCAGATATACATGATAGATATGGAAAAATTCAATTATACATAAAGATAAATGATGTAGGGGAAGAAAAACTTAAAGAATA
TAAGACTTTTGATATAGGTGATATAATAAGTGTAACAGGAACAGTTTTTAAAACAAAAACAGGAGAAACATCAATACACA
TAACAGATTTTCAATTAGTTTGTAAATCTTTAAGACCATTACCAGAAAAGTGGCATGGATTAAAAGATCCAGATTTAAGA
TATAGACAAAGATATGTAGATTTAATAATAAACCAAGATGTTAGAGATACTTTTATAAAAAGAACAGCTATAATTAAGTC
TATGAGAGAATTCTTGGATAATAGAGGATTTTTAGAAGTAGAAACACCAATATTATCACCAATAGCAGGCGGGGCAGCAG
CTAAGCCTTTTATTACTCATCATAATGCTTTAGATATAGATATGTATCTTAGAATAGCTACTGAATTATATCTAAAAAGA
TTAATAGTAGGTGGCTTTGAAAAAGTATATGAGATTGGTAAAAACTTTAGAAATGAAGGAATAGATATAAGACATAATCC
AGAATTTACAGCTATAGAATTATATGAAGCTTATGCGGATTATAATGATATGATGGAAATCACAGAAAATATGATAGCTT
ATATATGCGAAAAAGTTCTAGGTACAACTAAAGTTGAATATGAAGGAGCAGAAATAGATTTTACACCACCATGGAGAAGA
TTAACTATGGTAGATGCTGTAAAGGAATATGCAGGAGTAGATTTTAACATTATAAAAAATGACATCGAGGCAAGAACAAT
CGCAAAAGAAAAACATATAGAATTTAAAAAAGAATTAAAGGATTGCACAAAAGGTGATGTACTTATAGGATTATTTGAGG
AATTCTGTGAAGACAAGTTAATGCAACCTACTTTTATTTGTGATTATCCAGTAGAAAATTCACCTCTTACAAAGAAGAAA
AGAGGAAATGAAGCATTTACTGAAAGATTTGAAGGCTTTGTATTTGGAAGAGAAGTATGTAACGCATATTCTGAATTAAA
TGATTCTATAGTTCAAAAAGAAAGATTCATGCAACAATTAAAGGAAAGAGAATTAGGTGATGATGAAGCTTATATGATGG
ATGACGACTTTATAACTTCATTAGAAGTAGGTATGCCACCGACAGGAGGATTAGGAATAGGTATTGATAGATTAATAATG
TTCTTAACAGATACACATTCTATAAGAGATGTTATACTATTCCCAACTATGAAACCACAACCTAATAATCAATAA

Upstream 100 bases:

>100_bases
TAAAAAAGTTGGAGATATAATAGAAGTAACAGTTCCAGATGGTGTTAGTAAATATGAAATATTAGAGGTTAACAGAGCAT
AGTATTGGAGGGAGTTTATA

Downstream 100 bases:

>100_bases
ATTTAAAATCTATAAATATATTAGTTACATTTGAAGATGAATAGTTTATATATGAATAGCAAATCATTATAAATAACAAC
AAGAATAAATTAAAAGCAAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYENLEGKTVTVAGRLMSKRVHGKAGF
SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR
YRQRYVDLIINQDVRDTFIKRTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKR
LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVLGTTKVEYEGAEIDFTPPWRR
LTMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK
RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
FLTDTHSIRDVILFPTMKPQPNNQ

Sequences:

>Translated_504_residues
MSKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYENLEGKTVTVAGRLMSKRVHGKAGF
SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR
YRQRYVDLIINQDVRDTFIKRTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKR
LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVLGTTKVEYEGAEIDFTPPWRR
LTMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK
RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
FLTDTHSIRDVILFPTMKPQPNNQ
>Mature_503_residues
SKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYENLEGKTVTVAGRLMSKRVHGKAGFS
DIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRY
RQRYVDLIINQDVRDTFIKRTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKRL
IVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVLGTTKVEYEGAEIDFTPPWRRL
TMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKR
GNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIMF
LTDTHSIRDVILFPTMKPQPNNQ

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=518, Percent_Identity=44.2084942084942, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI5031815, Length=518, Percent_Identity=44.2084942084942, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI45439306, Length=468, Percent_Identity=23.2905982905983, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI40789249, Length=265, Percent_Identity=26.7924528301887, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI1790571, Length=498, Percent_Identity=50.2008032128514, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789256, Length=494, Percent_Identity=49.5951417004049, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=26.7692307692308, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788173, Length=307, Percent_Identity=25.0814332247557, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=509, Percent_Identity=42.8290766208251, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535927, Length=509, Percent_Identity=42.8290766208251, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=42.8571428571429, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551876, Length=508, Percent_Identity=23.6220472440945, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=303, Percent_Identity=25.0825082508251, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=466, Percent_Identity=43.7768240343348, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324256, Length=481, Percent_Identity=33.0561330561331, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=523, Percent_Identity=25.2390057361377, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325153, Length=153, Percent_Identity=28.7581699346405, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640851, Length=531, Percent_Identity=44.4444444444444, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24640849, Length=531, Percent_Identity=44.4444444444444, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24584738, Length=351, Percent_Identity=25.0712250712251, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58272; Mature: 58141

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYENLEG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHCHHHHHHHHHCCCC
KTVTVAGRLMSKRVHGKAGFSDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTG
CEEEEHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCC
TVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRYRQRYVDLIINQDVRDTFIK
CEEEECCCCCEEEEEHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
RTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKR
HHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
GTTKVEYEGAEIDFTPPWRRLTMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELK
CCCEEEECCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKRGNEAFTERFEGFVFGREVC
HCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHEEECCCCCCCCCCCCHHHHHH
FLTDTHSIRDVILFPTMKPQPNNQ
HHHCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
SKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYENLEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHCHHHHHHHHHCCCC
KTVTVAGRLMSKRVHGKAGFSDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTG
CEEEEHHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCHHHHHHCC
TVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLRYRQRYVDLIINQDVRDTFIK
CEEEECCCCCEEEEEHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
RTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKR
HHHHHHHHHHHHHCCCEEEECCCHHCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
GTTKVEYEGAEIDFTPPWRRLTMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELK
CCCEEEECCCEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKKRGNEAFTERFEGFVFGREVC
HCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
NAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHEEECCCCCCCCCCCCHHHHHH
FLTDTHSIRDVILFPTMKPQPNNQ
HHHCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA