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The map label for this gene is buk [H]

Identifier: 226950878

GI number: 226950878

Start: 3933429

End: 3934499

Strand: Reverse

Name: buk [H]

Synonym: CLM_3892

Alternate gene names: 226950878

Gene position: 3934499-3933429 (Counterclockwise)

Preceding gene: 226950879

Following gene: 226950877

Centisome position: 94.69

GC content: 32.31

Gene sequence:

>1071_bases
ATGGGTTACAAATTGTTAATAATTAATCCCGGGTCAACATCAACTAAAATAGGTGTATATGAAGATGAGAATCAAATATT
GGAAGAAACATTAAGACATTCTTCACAAGAAATTGAAAGCTATAAAACTATATTTGATCAATTTGAATTTAGAAAAGAAG
TTATATTAAATGTTTTAAAAGAGAAAAGCTTTGATGTAAATGAATTAGATGCTATTGTAGGCAGAGGTGGACTTTTAAAA
CCTATTGAAGGCGGAACTTACAGAGTAAATGACGTCATGATTGAAGATTTAAAAATAGGGGTTCAAGGACAACATGCATC
TAATCTTGGAGGAATAATAGCTAATGAAATAGGAAAAAATCTTAACATACCTGCATTTATAGTAGATCCTGTAGTTGTTG
ATGAAATGAAGGATATAGCAAGAATATCAGGTATGCCTGAAATAGAGAGAAAAAGTATATTTCATGCATTAAATCAAAAG
GCAGTTGCTAAAAGATACGCAAAAGAAAACAATAAAAAATATGAGGAGCTTAATTTAATAGTTACACACATGGGTGGAGG
GGCTTCTGTAGGAACTCACGAAAAAGGAAAGGTAGTAGATGTAAATAATGCTTTAGATGGAGAAGGACCATTTTCACCAG
AAAGGGCAGGCGGTCTTCCAGTAGGAGATCTAGTGAAATTATGCTACAGTGGTAAGTACACTTATGAAGAAATGAAAAAG
AAGATAAGCGGTAAAGGTGGAGTAGTAGCATATTTAAATACAAATGATTTTAGAGAAGTTGAAGAAAGAGCTGAAAAAGG
AGATAAAGATGCAAAATTAATTGTTGATGCATTTATATTTCAAATAGCAAAAGAAATAGGAAAAAATGCTGCTGTTTTAT
GTGGAAAAGTAGACGCAATATTATTAACAGGGGGAATAGCATATAGTAAAGCTATATGTGAGGGTATAAAAGGAATGGTA
TCTTTTATAGCACCAGTAGTTAGATTCCCAGGAGAAGATGAGTTATTAGCATTAGCTCAAGGTGGATTAAGAGTACTAAA
AGGAGAAGAAGAGGCTAAAGAATACAAATAG

Upstream 100 bases:

>100_bases
AGTGATAGCCATGAATCAAAACTAAATGCAATAGCTTTAGCAGCATTAGTTGCTAGCCAACTAAAAAAATAAATATCTTA
AATTTAGGGAGGAACTTAAA

Downstream 100 bases:

>100_bases
TGTCAATTTTAGAATGTAGATATAAATAGAATAATTAGAAAAATAAAGAATTTTTTTTATGATAAAATTATAACAATTAA
AAAAAATAGAAAATTGCTTT

Product: butyrate kinase

Products: NA

Alternate protein names: BK [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MGYKLLIINPGSTSTKIGVYEDENQILEETLRHSSQEIESYKTIFDQFEFRKEVILNVLKEKSFDVNELDAIVGRGGLLK
PIEGGTYRVNDVMIEDLKIGVQGQHASNLGGIIANEIGKNLNIPAFIVDPVVVDEMKDIARISGMPEIERKSIFHALNQK
AVAKRYAKENNKKYEELNLIVTHMGGGASVGTHEKGKVVDVNNALDGEGPFSPERAGGLPVGDLVKLCYSGKYTYEEMKK
KISGKGGVVAYLNTNDFREVEERAEKGDKDAKLIVDAFIFQIAKEIGKNAAVLCGKVDAILLTGGIAYSKAICEGIKGMV
SFIAPVVRFPGEDELLALAQGGLRVLKGEEEAKEYK

Sequences:

>Translated_356_residues
MGYKLLIINPGSTSTKIGVYEDENQILEETLRHSSQEIESYKTIFDQFEFRKEVILNVLKEKSFDVNELDAIVGRGGLLK
PIEGGTYRVNDVMIEDLKIGVQGQHASNLGGIIANEIGKNLNIPAFIVDPVVVDEMKDIARISGMPEIERKSIFHALNQK
AVAKRYAKENNKKYEELNLIVTHMGGGASVGTHEKGKVVDVNNALDGEGPFSPERAGGLPVGDLVKLCYSGKYTYEEMKK
KISGKGGVVAYLNTNDFREVEERAEKGDKDAKLIVDAFIFQIAKEIGKNAAVLCGKVDAILLTGGIAYSKAICEGIKGMV
SFIAPVVRFPGEDELLALAQGGLRVLKGEEEAKEYK
>Mature_355_residues
GYKLLIINPGSTSTKIGVYEDENQILEETLRHSSQEIESYKTIFDQFEFRKEVILNVLKEKSFDVNELDAIVGRGGLLKP
IEGGTYRVNDVMIEDLKIGVQGQHASNLGGIIANEIGKNLNIPAFIVDPVVVDEMKDIARISGMPEIERKSIFHALNQKA
VAKRYAKENNKKYEELNLIVTHMGGGASVGTHEKGKVVDVNNALDGEGPFSPERAGGLPVGDLVKLCYSGKYTYEEMKKK
ISGKGGVVAYLNTNDFREVEERAEKGDKDAKLIVDAFIFQIAKEIGKNAAVLCGKVDAILLTGGIAYSKAICEGIKGMVS
FIAPVVRFPGEDELLALAQGGLRVLKGEEEAKEYK

Specific function: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate [H]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=165, Percent_Identity=30.9090909090909, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693184, Length=165, Percent_Identity=24.8484848484848, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38940; Mature: 38809

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYKLLIINPGSTSTKIGVYEDENQILEETLRHSSQEIESYKTIFDQFEFRKEVILNVLK
CCEEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKSFDVNELDAIVGRGGLLKPIEGGTYRVNDVMIEDLKIGVQGQHASNLGGIIANEIGKN
HCCCCHHHHHHHHCCCCCCCCCCCCCEEEHHEEEEHHHCCCCCCCCCHHCHHHHHHHCCC
LNIPAFIVDPVVVDEMKDIARISGMPEIERKSIFHALNQKAVAKRYAKENNKKYEELNLI
CCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
VTHMGGGASVGTHEKGKVVDVNNALDGEGPFSPERAGGLPVGDLVKLCYSGKYTYEEMKK
EEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
KISGKGGVVAYLNTNDFREVEERAEKGDKDAKLIVDAFIFQIAKEIGKNAAVLCGKVDAI
HHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
LLTGGIAYSKAICEGIKGMVSFIAPVVRFPGEDELLALAQGGLRVLKGEEEAKEYK
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCHHHHCCC
>Mature Secondary Structure 
GYKLLIINPGSTSTKIGVYEDENQILEETLRHSSQEIESYKTIFDQFEFRKEVILNVLK
CEEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKSFDVNELDAIVGRGGLLKPIEGGTYRVNDVMIEDLKIGVQGQHASNLGGIIANEIGKN
HCCCCHHHHHHHHCCCCCCCCCCCCCEEEHHEEEEHHHCCCCCCCCCHHCHHHHHHHCCC
LNIPAFIVDPVVVDEMKDIARISGMPEIERKSIFHALNQKAVAKRYAKENNKKYEELNLI
CCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
VTHMGGGASVGTHEKGKVVDVNNALDGEGPFSPERAGGLPVGDLVKLCYSGKYTYEEMKK
EEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
KISGKGGVVAYLNTNDFREVEERAEKGDKDAKLIVDAFIFQIAKEIGKNAAVLCGKVDAI
HHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
LLTGGIAYSKAICEGIKGMVSFIAPVVRFPGEDELLALAQGGLRVLKGEEEAKEYK
EEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEECCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]