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The map label for this gene is rumA [C]

Identifier: 226950817

GI number: 226950817

Start: 3868896

End: 3870281

Strand: Reverse

Name: rumA [C]

Synonym: CLM_3829

Alternate gene names: 226950817

Gene position: 3870281-3868896 (Counterclockwise)

Preceding gene: 226950818

Following gene: 226950816

Centisome position: 93.14

GC content: 28.79

Gene sequence:

>1386_bases
ATGAAAAAAAATATACCTGTAGAAAAAAATAAAGAATACAATTTAGACATAATAGGCACTGGATTTGAAGGAGAAGGGGT
AAGTAAAATTGATGATTTTACAGTATTTATTCCAGGAGCCATAAAAGGAGAAAATGCATTAACAAGAATAGTTAAAGTAA
ATAAAAACTTCGCCTTTGGAAAGATTATAAAGTTAAACAAAACATCAGAAAATAGAGTAGAATCTGTATGTCCTATATAT
AAACAGTGTGGAGGATGTCAAGTTCAACACTATAGTTATAAGGCTCAATTAGAGTTTAAAAAGCAGAGGGTTATAGATTC
ACTAGAAAGAATAGGTAAACTAGATATAGATAAAATAAAAATAGAAGATACTATTGGCATGGAAAACCCGTATAGATATA
GAAACAAAATTCAAATGCCTGTAAGATTTGAAAAAGGTGAAATAAAAATAGGATTTTATAAATCAAGAACTCATGAAGTA
GCAGATGTAAAAGAATGTTTTATACAAGATGAGCAAGCAGATAAAATTATACAAATAATAAGAAATTGGATGGTAAAACA
TAATATATCTGCCTACAATGAAGAAACAGGAAAAGGGTTTATAAGGCATATAATGGTAAGGAAAGCTTTTAAAAATAAAG
AACTTATGTTAGTTATTGTAACTAATAAAGAAGAGGAAATACCTTACAAAGAAGAATTAATAAATGAAGTAATAAAAGAA
TTTTCAAATATAAAAAGTATAATACAAAATATAAATACTAAAAAAACCAATGTGATTTTAGGAGAAAAGTGTATTACATT
ATGGGGAAAAGATACCATAACTGACTATATAGATAAGTTTAAATTTAATATATCTCCATTATCTTTTTTTCAGGTAAATC
CCATACAAACAGAGGTATTATATAAAAAAGCCTTAGAATTTGCAAATCTAAAAGGAGAAGAAATAGTATTTGATGCTTAC
TGTGGCACAGGAACTATATCCTTATTTTTATCACAGAAAGCTAAAAAAGTATACGGAGTAGAAATAATAAAGGAAGCTAT
AGACAACGCAAAAGATAATGCAAAAGAAAATAGGATAGATAATGCAGAATTTTTTGTAGGAAAATCAGAAGAAGTTATAC
CAGATTTAATAGATAAAGGTATAAAAGCAGAGGTGGTAGTTGTAGATCCACCAAGAAAAGGCTGCGAAAAAACCTTACTA
GAAGCTATAGCAGCTACAAAACCTAAAAGAATAGTATATGTATCCTGTGATTCAGGAACTTTGGCTAGAGACTTAGGTAT
ATTAAATGGATTAGGTTATGAAACTAAAGAAGTACAACCAGTAGATATGTTCCCCCAAACTAGCCATGTGGAGACTATTG
TGTTGATACAACGAGCCGATACGTAG

Upstream 100 bases:

>100_bases
AATTTATATATTGGAAAGAACCTAAGGATAGATAGTCTAATAGTTACTATTTATCCTGTGTTTTTGTTATGATATAAAAT
TACATAAATGGAGAGAGAGT

Downstream 100 bases:

>100_bases
AAATCCTTTGTTTTAGGCACTTCTTCAACCATTTTGTTTTTATAAATGAGGGTGATAAAATCCGTAATAAACAGCTAAAA
AAATATATTAACGTTTCTGT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKAEVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDSGTLARDLGILNGLGYETKEVQPVDMFPQTSHVETIVLIQRADT

Sequences:

>Translated_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKAEVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDSGTLARDLGILNGLGYETKEVQPVDMFPQTSHVETIVLIQRADT
>Mature_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRIDNAEFFVGKSEEVIPDLIDKGIKAEVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDSGTLARDLGILNGLGYETKEVQPVDMFPQTSHVETIVLIQRADT

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=352, Percent_Identity=28.4090909090909, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI51173878, Length=352, Percent_Identity=28.4090909090909, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI269784678, Length=284, Percent_Identity=26.4084507042254, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI269784680, Length=206, Percent_Identity=32.0388349514563, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI269784676, Length=206, Percent_Identity=32.0388349514563, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI20149688, Length=206, Percent_Identity=32.0388349514563, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789148, Length=437, Percent_Identity=27.4599542334096, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=25.5384615384615, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=166, Percent_Identity=35.5421686746988, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322909, Length=450, Percent_Identity=25.3333333333333, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24666579, Length=436, Percent_Identity=29.1284403669725, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52591; Mature: 52591

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRID
HHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
NAEFFVGKSEEVIPDLIDKGIKAEVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDSGT
CCEEEECCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
LARDLGILNGLGYETKEVQPVDMFPQTSHVETIVLIQRADT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEELINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENRID
HHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
NAEFFVGKSEEVIPDLIDKGIKAEVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDSGT
CCEEEECCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCC
LARDLGILNGLGYETKEVQPVDMFPQTSHVETIVLIQRADT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]