BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is ytbE [H]

Identifier: 226950806

GI number: 226950806

Start: 3860530

End: 3861375

Strand: Reverse

Name: ytbE [H]

Synonym: CLM_3817

Alternate gene names: 226950806

Gene position: 3861375-3860530 (Counterclockwise)

Preceding gene: 226950807

Following gene: 226950805

Centisome position: 92.93

GC content: 39.01

Gene sequence:

>846_bases
ATGAAAGAAAATTTTTACTCATTAAGCAATAATTACAAGATTCCGAATATAGGATTCGGTACATTCCGTACCCCTAGTGG
AGAGGAAACAGAGCAGTCTGTATTAAATGCAATCAAGGCAGGATATAGGCATATTGACTGCGCTGCTGCTTATGGAAATG
AAAAAAGTGTTGGTGAAGCCATACGTAAATCCGGTGTAGCACGCGAAGAATTATTTGTAACAAGTAAGCTATGGAATGAT
GACAAAGGATATGAAAAGACGTTAGCTGCCTTTAATCGAACCTTAGAGGATTTACAGCTTGATTATTTGGATTTATACCT
TATTCACTGGCCTATTGCAAAAGCATCTAAGAATAACTGGCAGGAAGCAAACAGCGAGAGCTGGAGAGCATTCGAGGAAC
TTTATAAACAGGGTAAAGTTAAAGCAATCGGAGTTAGTAATTTTCTTCCGCACCATTTTGAGCCATTGTTGGCAATCACT
AAAATCCAACCGATGGTGAATCAAATTGAATTCCATCCTGGCATGTTGCAGGAGGAAACCGTAGCGTCTTGTAAGCAACG
CAATATTTTGGTGGAGGCATGGGCTCCATTTTCAAACGGACAGATTTTGGAGCATCCGGTATTAAAGGAAATTGCGGATC
AATACAAAAAATCCGTAGCTCAGCTTAGTCTGCGCTGGATTATTCAAAAAGGAATTATTCCATTGCCAAAGTCTGTTACA
CCGGAGAGAATTAAGAATAATCTTAAGGTATTTGACTTTGAAATTAGTGCACAAGATGTTGAGAAGATTGACAGACTAAC
CGATTGTGGAAGTTCAGGGCTTCATCCAGATAAAGTAGATTTCTAA

Upstream 100 bases:

>100_bases
AGACAATTGACGCTGGAAAAGAGAAAATACCTGTTCCTGTAGAAGAAGACCATGTTATGACTAATTTTATACGATAATAA
ATCAAAAAGGAAAATAATCT

Downstream 100 bases:

>100_bases
GATTATCCAATATCTATCACTGTTAAGAAAGTTATTCCTTAAACCGTTGAATAAGTGAATATTAAGAACATGAATAGAGT
GCATTTCTTGAACAAGGGAT

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSGVAREELFVTSKLWND
DKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAIT
KIQPMVNQIEFHPGMLQEETVASCKQRNILVEAWAPFSNGQILEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLPKSVT
PERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF

Sequences:

>Translated_281_residues
MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSGVAREELFVTSKLWND
DKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAIT
KIQPMVNQIEFHPGMLQEETVASCKQRNILVEAWAPFSNGQILEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLPKSVT
PERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF
>Mature_281_residues
MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEAIRKSGVAREELFVTSKLWND
DKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNWQEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAIT
KIQPMVNQIEFHPGMLQEETVASCKQRNILVEAWAPFSNGQILEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLPKSVT
PERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=39.5904436860068, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=39.5904436860068, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=41.7241379310345, Blast_Score=206, Evalue=1e-53,
Organism=Homo sapiens, GI93277124, Length=291, Percent_Identity=36.426116838488, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4502049, Length=286, Percent_Identity=43.7062937062937, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI291291012, Length=265, Percent_Identity=39.622641509434, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=36.2007168458781, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI310109920, Length=291, Percent_Identity=35.0515463917526, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI5453543, Length=291, Percent_Identity=35.0515463917526, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI5174695, Length=292, Percent_Identity=36.3013698630137, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI45446745, Length=291, Percent_Identity=34.7079037800687, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI4503285, Length=291, Percent_Identity=34.7079037800687, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=33.6700336700337, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=34.3642611683849, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI300116273, Length=276, Percent_Identity=36.9565217391304, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=32.9749103942652, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI310109926, Length=113, Percent_Identity=45.1327433628319, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=44.2477876106195, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310109924, Length=150, Percent_Identity=32, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87082198, Length=273, Percent_Identity=39.1941391941392, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=34.3396226415094, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788081, Length=282, Percent_Identity=31.5602836879433, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994888, Length=240, Percent_Identity=24.5833333333333, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=261, Percent_Identity=41.3793103448276, Blast_Score=200, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17537077, Length=303, Percent_Identity=37.2937293729373, Blast_Score=190, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17537075, Length=284, Percent_Identity=37.3239436619718, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=34.5724907063197, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17564128, Length=297, Percent_Identity=34.006734006734, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=34.982332155477, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=34.0909090909091, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17550248, Length=282, Percent_Identity=35.4609929078014, Blast_Score=154, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17537079, Length=292, Percent_Identity=31.1643835616438, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17562292, Length=255, Percent_Identity=29.8039215686275, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=30.9963099630996, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322556, Length=286, Percent_Identity=37.4125874125874, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324694, Length=287, Percent_Identity=38.6759581881533, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320576, Length=270, Percent_Identity=41.1111111111111, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321896, Length=305, Percent_Identity=34.7540983606557, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320079, Length=287, Percent_Identity=34.1463414634146, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=299, Percent_Identity=33.1103678929766, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=40.7665505226481, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24662789, Length=293, Percent_Identity=37.2013651877133, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=38.5135135135135, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21356425, Length=281, Percent_Identity=37.7224199288256, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=35.7594936708861, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24663317, Length=282, Percent_Identity=34.0425531914894, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20129731, Length=278, Percent_Identity=34.1726618705036, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24662785, Length=270, Percent_Identity=41.4814814814815, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24662781, Length=275, Percent_Identity=40.3636363636364, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553081, Length=278, Percent_Identity=30.9352517985612, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI281366140, Length=245, Percent_Identity=31.0204081632653, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31988; Mature: 31988

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCHHHHHH
IRKSGVAREELFVTSKLWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNW
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
QEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAITKIQPMVNQIEFHPGMLQEET
HHCCCHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VASCKQRNILVEAWAPFSNGQILEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLPKSVT
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF
HHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKENFYSLSNNYKIPNIGFGTFRTPSGEETEQSVLNAIKAGYRHIDCAAAYGNEKSVGEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCHHHHHH
IRKSGVAREELFVTSKLWNDDKGYEKTLAAFNRTLEDLQLDYLDLYLIHWPIAKASKNNW
HHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
QEANSESWRAFEELYKQGKVKAIGVSNFLPHHFEPLLAITKIQPMVNQIEFHPGMLQEET
HHCCCHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VASCKQRNILVEAWAPFSNGQILEHPVLKEIADQYKKSVAQLSLRWIIQKGIIPLPKSVT
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PERIKNNLKVFDFEISAQDVEKIDRLTDCGSSGLHPDKVDF
HHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]