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The map label for this gene is azoB [H]

Identifier: 226950805

GI number: 226950805

Start: 3859204

End: 3860058

Strand: Reverse

Name: azoB [H]

Synonym: CLM_3816

Alternate gene names: 226950805

Gene position: 3860058-3859204 (Counterclockwise)

Preceding gene: 226950806

Following gene: 226950803

Centisome position: 92.9

GC content: 32.87

Gene sequence:

>855_bases
ATGAATATAATATTAGGAGCAACAGGACAGATAGGGACAATGCTTGTTGATAATCTTTTAGAGCAAGGTCAACCAGTAAA
AGCTGTTATTCGGAATTATTCTAAAGCACAAGAATTAAAAAATAAAGGTGTTGAGGTTTTTATTACAGATTCTTTTGAAG
TAGAAGCCTTAAAAAAGGCCTTTCATGGGGGAAGTTCAGTTTTCCTTTTAACACCTGAAAATCGTAAATGTGAAAATTTT
TTAAACGAAACTCAAATGATTATAAATAATTATAGAAAGGCTATTTTATCATCAGGAATTACGAAAATTGTTGGGTTATC
TTCTGTTGGTGCACAGCACAAATCTGAAACAGGTAATCTGATAGCATCTTATATGTTGGAACACGCCTTCTCCGATCTTG
AAATTGAACAAATCTTTATTCGACCTACCTATTATTTTAGTAATTGGCTTGGATATTTAGAATTGGTTAAGGAGCATGGA
ATACTGCCAACTTTTTTTCCACCTGGAATGGAGTTACCCATGATTGCTCCATCAGATGTTGCGAAATTTTTATCAAAGGT
AATGCTTTGTGAAACTCCGCAGGAAAAAATGTATGAAATAACTGGACCACACTCTTACAGTTCTTCAGATATGGCAAAAA
TATTTGAAGATGTATTGAATAGGGAGGTCATTTTACAGCAACTATTACCAGAAGAATGGGAAAGTACATTAATACAAGCA
GGGTTCTCAATAGACGGAGCAAAAAATTTTATGCTTATGACAAAAGCAGTTATTGATGGAAAAACTAAATATGATACGAC
TAATCCGATTCCCTTTTCTACAGATTTCAAAAAGTATTTGAAATCTGTTATATAA

Upstream 100 bases:

>100_bases
GGGCCTGCACTAGCTGGGTAGCAAGATAGGAATCATCGTTCACGCGATTCTCCATGTTTGCGATTCCAATTTTATGCTAA
TAAAACACTGGAGGTATAAT

Downstream 100 bases:

>100_bases
AAACTTTCATTGAGGATTATTTATAAATTATCGAGCGTAATTGGGTAAATTAGCAGAGCCAACAGAATGCTCCGCTAAAA
GCATTGAATTTTAAGCAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNIILGATGQIGTMLVDNLLEQGQPVKAVIRNYSKAQELKNKGVEVFITDSFEVEALKKAFHGGSSVFLLTPENRKCENF
LNETQMIINNYRKAILSSGITKIVGLSSVGAQHKSETGNLIASYMLEHAFSDLEIEQIFIRPTYYFSNWLGYLELVKEHG
ILPTFFPPGMELPMIAPSDVAKFLSKVMLCETPQEKMYEITGPHSYSSSDMAKIFEDVLNREVILQQLLPEEWESTLIQA
GFSIDGAKNFMLMTKAVIDGKTKYDTTNPIPFSTDFKKYLKSVI

Sequences:

>Translated_284_residues
MNIILGATGQIGTMLVDNLLEQGQPVKAVIRNYSKAQELKNKGVEVFITDSFEVEALKKAFHGGSSVFLLTPENRKCENF
LNETQMIINNYRKAILSSGITKIVGLSSVGAQHKSETGNLIASYMLEHAFSDLEIEQIFIRPTYYFSNWLGYLELVKEHG
ILPTFFPPGMELPMIAPSDVAKFLSKVMLCETPQEKMYEITGPHSYSSSDMAKIFEDVLNREVILQQLLPEEWESTLIQA
GFSIDGAKNFMLMTKAVIDGKTKYDTTNPIPFSTDFKKYLKSVI
>Mature_284_residues
MNIILGATGQIGTMLVDNLLEQGQPVKAVIRNYSKAQELKNKGVEVFITDSFEVEALKKAFHGGSSVFLLTPENRKCENF
LNETQMIINNYRKAILSSGITKIVGLSSVGAQHKSETGNLIASYMLEHAFSDLEIEQIFIRPTYYFSNWLGYLELVKEHG
ILPTFFPPGMELPMIAPSDVAKFLSKVMLCETPQEKMYEITGPHSYSSSDMAKIFEDVLNREVILQQLLPEEWESTLIQA
GFSIDGAKNFMLMTKAVIDGKTKYDTTNPIPFSTDFKKYLKSVI

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31901; Mature: 31901

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIILGATGQIGTMLVDNLLEQGQPVKAVIRNYSKAQELKNKGVEVFITDSFEVEALKKA
CEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
FHGGSSVFLLTPENRKCENFLNETQMIINNYRKAILSSGITKIVGLSSVGAQHKSETGNL
HCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCHHHHHH
IASYMLEHAFSDLEIEQIFIRPTYYFSNWLGYLELVKEHGILPTFFPPGMELPMIAPSDV
HHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AKFLSKVMLCETPQEKMYEITGPHSYSSSDMAKIFEDVLNREVILQQLLPEEWESTLIQA
HHHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
GFSIDGAKNFMLMTKAVIDGKTKYDTTNPIPFSTDFKKYLKSVI
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MNIILGATGQIGTMLVDNLLEQGQPVKAVIRNYSKAQELKNKGVEVFITDSFEVEALKKA
CEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
FHGGSSVFLLTPENRKCENFLNETQMIINNYRKAILSSGITKIVGLSSVGAQHKSETGNL
HCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCHHHHHH
IASYMLEHAFSDLEIEQIFIRPTYYFSNWLGYLELVKEHGILPTFFPPGMELPMIAPSDV
HHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
AKFLSKVMLCETPQEKMYEITGPHSYSSSDMAKIFEDVLNREVILQQLLPEEWESTLIQA
HHHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
GFSIDGAKNFMLMTKAVIDGKTKYDTTNPIPFSTDFKKYLKSVI
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]