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The map label for this gene is proS [H]

Identifier: 226950778

GI number: 226950778

Start: 3827676

End: 3829112

Strand: Reverse

Name: proS [H]

Synonym: CLM_3789

Alternate gene names: 226950778

Gene position: 3829112-3827676 (Counterclockwise)

Preceding gene: 226950779

Following gene: 226950777

Centisome position: 92.15

GC content: 34.03

Gene sequence:

>1437_bases
ATGGCAGGAGATAAAAAATTTGTTGAAGATATTACACCAATGGATGAAGACTTTGCACAATGGTATACGGATATAGTAAA
AAAGGCAGAGCTTGCTGATTACTCAAGTATCAGAGGATGTATGATTATTCGTCCTAATGGTTATGCAATTTGGGAAAATA
TACAGAAATATGTAGATACAAAACTTAAAGAGTATGGACATGAAAATGTATCTATGCCAATATTTATACCTGAAAACTTA
TTACAAAAAGAAAAAGATCACGTTGAAGGATTTGCACCTGAAGTTGCATGGGTAACTCATGGTGGAGATGACGAATTAGC
TGAAAGATTATGTGTTCGTCCAACATCAGAAACATTGTTCTGTGAACACTATGCTAAGATTGTTCAATCTTATAAAGATT
TACCAAAGCTTTATAACCAATGGTGTTCAGTTGTAAGATGGGAAAAGACAACTAGACCTTTTTTAAGAACAACTGAATTC
TTATGGCAAGAAGGTCATACAATTCATGAAACAAAAGAAGAAGCAGAATCTCATTCACTTAAGATACTAAACATGTATTC
TAGATTATGTGAAGATATGTTAGCAATGCCAGTGGTTATGGGTAAAAAGACAGATAAGGAAAAATTTGCTGGTGCAGATG
ATACGTACACAATTGAAAGTTTAATGCATGATGGAAAGGCACTGCAAGCAGGTACTTCACACTATCTTGGACAAAATTTC
TCAAAGGCATTTGCAATTCAGTTCTCAGATAGAAATGGAAAGTTAGATTATCCACACTATACTACTTGGGCAGTAACTAC
AAGATTAATTGGAGCAATAATAATGGTCCATGGTGATAATAGTGGTCTAAAATTACCACCAAGAATTGCACCAACTCAAG
CGGTGATTATTCCTGTTGCTCAACATAAAGAAGGAGTACTGGAAAAAGCGAAAGAATTAAAGGAAAAATTGGCTAAGGTT
GTAAGAGTTAAGCTTGATGATAGCGATAAGATGCCAGGTTGGAAGTATAGTGAATATGAAATGAAGGGTATCCCACTAAG
AATAGAAATTGGGCCAAAGGATATAGAAAAAAACCAAGCTGTGTTAGTAAGAAGAGACAATAGAGAAAAAACAATAGTTT
CATTGGACGAAATTGAAATAAAGGTTCAAGAGATGTTAGATATTATCCATAATTCAATGCTAGAAGAAGCTAAAAAAACT
AGAGATGAAAAAACTTATGTAGCAACAAATATGGAAGAATTTGAAGATACTATTGAAAATAAACCAGGTTTTATTAAAGC
AATGTGGTGTGGAGATAGAGCTTGTGAAGATAAGATAAGAGAGGTAACTGGCGCTACATCTCGTTGTATGCCATTTGAAC
AAGAGGTTGTTTCAGACACTTGTGTATGTTGTGGTAAAAAAGCTAAAAATTTGGTTTACTGGGGAAGAGCATATTAA

Upstream 100 bases:

>100_bases
ATGTTAACAAGGGTGGTACCACCGACACAACTTCGGTCCCTGTATAGGGATTAGGAGTTTTTTTATTTACAAAAAAATAA
ATTTTAGGGAGGAATTTAAT

Downstream 100 bases:

>100_bases
TTAGAAGTTATTTAATTAATAGATTTTATAAAATAAATATTAGAAAATTAAAGGGTATAGGGGAATCAATGATTTTTCTA
TACCTTTTATATAGTGGGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Sequences:

>Translated_478_residues
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENL
LQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEF
LWQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKV
VRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKT
RDEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
>Mature_477_residues
AGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDTKLKEYGHENVSMPIFIPENLL
QKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLFCEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFL
WQEGHTIHETKEEAESHSLKILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNFS
KAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVAQHKEGVLEKAKELKEKLAKVV
RVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQAVLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTR
DEKTYVATNMEEFEDTIENKPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=44.421906693712, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1786392, Length=159, Percent_Identity=27.6729559748428, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=40, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=44.1340782122905, Blast_Score=344, Evalue=5e-95,
Organism=Saccharomyces cerevisiae, GI6321808, Length=497, Percent_Identity=45.6740442655936, Blast_Score=445, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24649468, Length=494, Percent_Identity=42.914979757085, Blast_Score=403, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24649466, Length=494, Percent_Identity=42.914979757085, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 54935; Mature: 54804

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDT
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAKELKEKLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure 
AGDKKFVEDITPMDEDFAQWYTDIVKKAELADYSSIRGCMIIRPNGYAIWENIQKYVDT
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHCCCEEEECCCCCHHHHHHHHHHHH
KLKEYGHENVSMPIFIPENLLQKEKDHVEGFAPEVAWVTHGGDDELAERLCVRPTSETLF
HHHHCCCCCCCEEEEECHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCCCHHH
CEHYAKIVQSYKDLPKLYNQWCSVVRWEKTTRPFLRTTEFLWQEGHTIHETKEEAESHSL
HHHHHHHHHHHHHHHHHHHHHHHHEECCHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KILNMYSRLCEDMLAMPVVMGKKTDKEKFAGADDTYTIESLMHDGKALQAGTSHYLGQNF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHCCCHHHHCCCC
SKAFAIQFSDRNGKLDYPHYTTWAVTTRLIGAIIMVHGDNSGLKLPPRIAPTQAVIIPVA
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCEEEEECH
QHKEGVLEKAKELKEKLAKVVRVKLDDSDKMPGWKYSEYEMKGIPLRIEIGPKDIEKNQA
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCE
VLVRRDNREKTIVSLDEIEIKVQEMLDIIHNSMLEEAKKTRDEKTYVATNMEEFEDTIEN
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCC
KPGFIKAMWCGDRACEDKIREVTGATSRCMPFEQEVVSDTCVCCGKKAKNLVYWGRAY
CCCEEEEEECCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA