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The map label for this gene is gltD [C]

Identifier: 226950741

GI number: 226950741

Start: 3776226

End: 3777482

Strand: Reverse

Name: gltD [C]

Synonym: CLM_3750

Alternate gene names: 226950741

Gene position: 3777482-3776226 (Counterclockwise)

Preceding gene: 226950742

Following gene: 226950738

Centisome position: 90.91

GC content: 30.15

Gene sequence:

>1257_bases
ATGAAGGAAATAGATTTAGTAATTATAGGCGGAGGACCAGCAGGAATGGCTGCTGCAATTTCAGCTAAGAATAAAGGTAT
TGATGATATTTTAATATTAGAAAGAGAAGATTCTTTAGGTGGTATACTTAACCAATGCATCCATACAGGCTTTGGATTGC
ATGTATTTAAAGAAAATTTATCTGGTCCAGAATATGCCCAAAGATTGATAAATGAAATAAATAGATTAGAGATAGAATAT
AAAACAGATACAATGGTTTTATCTATAGATAAGGATAAGATTATCAAAGCAGTTAATCCTGAAGATGGAATGTTTGGTAT
AAAAGCTAAAGCTATTATACTAGCTTCAGGATGTAGAGAAAGACCTAAAGGAGCAATAAACATACCAGGAAGTAAGTGCG
CAGGTATATATACAGCAGGTTCGATACAGAAGTTTATAAATATAGAAGGGTATATGCCTGGCAAAGAGGTAGTTATATTA
GGTTCTGGGGATGTAGCTTTAGTAATGGCTAGAAGAATGGCTTTAGAAGGAGCAAATATAAAAGCTGTAGTTGAAATTAT
GCCTTATTGTTATGGAACAGAAAATAATGTGGTGAATTGTTTAGATGAATTTGATATACCACTCAAGTTAGGTCATACTG
TTGTAAATATAAAAGGAAAAGATAGAGTTGAGGGAGTTACCATAGCAAGAGTAGATGAAGATAGAATTCCTATACCAGGT
ACGGAAATGTATATACCTTGTGATACTCTAGTATTATCTGTAGAACTTAGGGGAGATAGTGAAATTATAAGAAAAACAGG
CATAGAATTAAATAAATCTACCTGGGGGCCTAAGGTGGGAGAAAATTTTCAAACCATGGTAGAAGGCATATTTGCTTGTG
GTAATGTTCTACATGTATATGATACTGCAGATAATGTGACAAAAGAGGGGTATAGCGTAGGAGAAAATGCTTCTCAATAT
ATTAAAGGGAAAAGCTTTTTAGAAGGTGAAAGAGTAGGATTATGGGCAGATAAAGGTATAAGATATTTGTTACCTAATTT
TATAAATTTACATAATTTAAAAGAAGATGTACAGTTAAAATTAAAATTTGATAAATTATATGAGAATCAGAATATTTATA
TTTATTTTGATAAAGCTAAAGAAAAATTAAGTTCAAATGAAAATATAAAACCTGGAGATATTATTAACATAAAAATATCT
AAAAATAAATTATTAAGCTATAAAGATTTAAAAAAGATAATTATAGGGATAAAATAA

Upstream 100 bases:

>100_bases
CAGTTCTACTTTAATTTTATTTTCTTTGATATATATGATAAAATTATATAGGAATTAAATCTAAATTCATATTAATAGCA
TTGTGCTGGAGGGTCTAATA

Downstream 100 bases:

>100_bases
AAAGATGAGCTAAAATAGCCTTAATTTTAAAACCGTAAATATAAATAATACATTCATAAAGCAATTCATTTTGCTATGAA
GTTCTAAATTTGACTTTATT

Product: pyridine nucleotide-disulfide oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: FAD-Dependent Pyridine Nucleotide-Disulfide Oxidoreductase; Sarcosine Oxidase Alpha Subunit; NAD(FAD)-Dependent Dehydrogenase; Oxidoreductase; NADH Oxidase; Sarcosine Oxidase Subunit Alpha; Pyridine Nucleotide-Disulfide Oxidoreductase; FAD Dependent Oxidoreductase; Pyridine Nucleotide-Disulphide Oxidoreductase; Proline Dehydrogenase Alpha Subunit; Pyridine Nucleotide-Disulfide Family Oxidoreductase; Pyridine Nucleotide-Disulfide Oxidoreductase Family Protein; Monooxygenase; Oxidase; tRNA Uridine 5-Carboxymethylaminomethyl GidA; Aminoacyl-TRNA Synthetase; Opine Oxidase; D-Nopaline Dehydrogenase; Aminomethyltransferase; Protein With Conserved CXXC Pairs; Dye-Linked L-Proline Dehydrogenase; Dye-L-ProDH Alpha

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKEIDLVIIGGGPAGMAAAISAKNKGIDDILILEREDSLGGILNQCIHTGFGLHVFKENLSGPEYAQRLINEINRLEIEY
KTDTMVLSIDKDKIIKAVNPEDGMFGIKAKAIILASGCRERPKGAINIPGSKCAGIYTAGSIQKFINIEGYMPGKEVVIL
GSGDVALVMARRMALEGANIKAVVEIMPYCYGTENNVVNCLDEFDIPLKLGHTVVNIKGKDRVEGVTIARVDEDRIPIPG
TEMYIPCDTLVLSVELRGDSEIIRKTGIELNKSTWGPKVGENFQTMVEGIFACGNVLHVYDTADNVTKEGYSVGENASQY
IKGKSFLEGERVGLWADKGIRYLLPNFINLHNLKEDVQLKLKFDKLYENQNIYIYFDKAKEKLSSNENIKPGDIINIKIS
KNKLLSYKDLKKIIIGIK

Sequences:

>Translated_418_residues
MKEIDLVIIGGGPAGMAAAISAKNKGIDDILILEREDSLGGILNQCIHTGFGLHVFKENLSGPEYAQRLINEINRLEIEY
KTDTMVLSIDKDKIIKAVNPEDGMFGIKAKAIILASGCRERPKGAINIPGSKCAGIYTAGSIQKFINIEGYMPGKEVVIL
GSGDVALVMARRMALEGANIKAVVEIMPYCYGTENNVVNCLDEFDIPLKLGHTVVNIKGKDRVEGVTIARVDEDRIPIPG
TEMYIPCDTLVLSVELRGDSEIIRKTGIELNKSTWGPKVGENFQTMVEGIFACGNVLHVYDTADNVTKEGYSVGENASQY
IKGKSFLEGERVGLWADKGIRYLLPNFINLHNLKEDVQLKLKFDKLYENQNIYIYFDKAKEKLSSNENIKPGDIINIKIS
KNKLLSYKDLKKIIIGIK
>Mature_418_residues
MKEIDLVIIGGGPAGMAAAISAKNKGIDDILILEREDSLGGILNQCIHTGFGLHVFKENLSGPEYAQRLINEINRLEIEY
KTDTMVLSIDKDKIIKAVNPEDGMFGIKAKAIILASGCRERPKGAINIPGSKCAGIYTAGSIQKFINIEGYMPGKEVVIL
GSGDVALVMARRMALEGANIKAVVEIMPYCYGTENNVVNCLDEFDIPLKLGHTVVNIKGKDRVEGVTIARVDEDRIPIPG
TEMYIPCDTLVLSVELRGDSEIIRKTGIELNKSTWGPKVGENFQTMVEGIFACGNVLHVYDTADNVTKEGYSVGENASQY
IKGKSFLEGERVGLWADKGIRYLLPNFINLHNLKEDVQLKLKFDKLYENQNIYIYFDKAKEKLSSNENIKPGDIINIKIS
KNKLLSYKDLKKIIIGIK

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.5.3.1

Molecular weight: Translated: 46158; Mature: 46158

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIDLVIIGGGPAGMAAAISAKNKGIDDILILEREDSLGGILNQCIHTGFGLHVFKENL
CCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
SGPEYAQRLINEINRLEIEYKTDTMVLSIDKDKIIKAVNPEDGMFGIKAKAIILASGCRE
CCHHHHHHHHHHHHHEEEEEECCEEEEEECHHHEEEECCCCCCEEEEEEEEEEEECCCCC
RPKGAINIPGSKCAGIYTAGSIQKFINIEGYMPGKEVVILGSGDVALVMARRMALEGANI
CCCCEEECCCCCCCCEEECCCHHHHEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCC
KAVVEIMPYCYGTENNVVNCLDEFDIPLKLGHTVVNIKGKDRVEGVTIARVDEDRIPIPG
EEEEEEHHHEECCCCCHHHHHHHCCCCEEECCEEEEECCCCCCCCEEEEEECCCCCCCCC
TEMYIPCDTLVLSVELRGDSEIIRKTGIELNKSTWGPKVGENFQTMVEGIFACGNVLHVY
CEEEEECCEEEEEEEECCCHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEE
DTADNVTKEGYSVGENASQYIKGKSFLEGERVGLWADKGIRYLLPNFINLHNLKEDVQLK
ECCCCHHHCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCEEEE
LKFDKLYENQNIYIYFDKAKEKLSSNENIKPGDIINIKISKNKLLSYKDLKKIIIGIK
EEEHHHHCCCCEEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKEIDLVIIGGGPAGMAAAISAKNKGIDDILILEREDSLGGILNQCIHTGFGLHVFKENL
CCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCC
SGPEYAQRLINEINRLEIEYKTDTMVLSIDKDKIIKAVNPEDGMFGIKAKAIILASGCRE
CCHHHHHHHHHHHHHEEEEEECCEEEEEECHHHEEEECCCCCCEEEEEEEEEEEECCCCC
RPKGAINIPGSKCAGIYTAGSIQKFINIEGYMPGKEVVILGSGDVALVMARRMALEGANI
CCCCEEECCCCCCCCEEECCCHHHHEEECCCCCCCCEEEECCCCHHHHHHHHHHHCCCCC
KAVVEIMPYCYGTENNVVNCLDEFDIPLKLGHTVVNIKGKDRVEGVTIARVDEDRIPIPG
EEEEEEHHHEECCCCCHHHHHHHCCCCEEECCEEEEECCCCCCCCEEEEEECCCCCCCCC
TEMYIPCDTLVLSVELRGDSEIIRKTGIELNKSTWGPKVGENFQTMVEGIFACGNVLHVY
CEEEEECCEEEEEEEECCCHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHCCCEEEEE
DTADNVTKEGYSVGENASQYIKGKSFLEGERVGLWADKGIRYLLPNFINLHNLKEDVQLK
ECCCCHHHCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCEEEE
LKFDKLYENQNIYIYFDKAKEKLSSNENIKPGDIINIKISKNKLLSYKDLKKIIIGIK
EEEHHHHCCCCEEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA